Role of Free Radicals and Metal Ions in Direct Current-Induced Cytotoxicity

Nakamura, Yuko; Takahashi, Keiso; Satoh, Kazue; Shimetani, Akiko; Sakagami, Hiroshi; Nishikawa, Hirofumi

doi:10.1016/j.joen.2005.11.010

Cited by 6 publications

(7 citation statements)

References 21 publications

(21 reference statements)

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“…Rutin, which was isolated by us before [24], was chosen as the positive control, and its purity was > 98 % as determined by HPLC. The MTT assay was carried out according to the methods of Mosmann and Nakamura et al [25,26] (see Supporting Information).…”

Section: Neutrophil Respiratory Burst Inhibitory Activitymentioning

confidence: 99%

Isolation and Anti-Inflammatory Activity Evaluation of Triterpenoids and a Monoterpenoid Glycoside fromHarpagophytum procumbens

Li²,

Zhou³

et al. 2010

Planta Med

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A new triterpenoid glycoside, designated harproside (1), and a new iridoid glycoside, named pagide (2), along with six known triterpenoids (3-8), were obtained from the tubers of Harpagophytum procumbens D. C. (devil's claw), and their structures were established through chemical methods and spectroscopic analyses. In an in vitro assay, the six triterpenoids showed anti-inflammatory activity. Compounds 3, 7, and 8 showed significant inhibitory activity against neutrophil respiratory burst stimulated by PMA, while compounds 4, 5, and 6 showed marginal inhibitory activity.

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Section: Neutrophil Respiratory Burst Inhibitory Activitymentioning

confidence: 99%

Isolation and Anti-Inflammatory Activity Evaluation of Triterpenoids and a Monoterpenoid Glycoside fromHarpagophytum procumbens

Li²,

Zhou³

et al. 2010

Planta Med

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“…1D) was the weakest among the 4 electrodes tested, in which NAC showed the lowest protective efficacy. We have already reported that iodine zinc iodide inhibited alkoxyl radical generation but not carbon radical (5), suggesting that the Zn ion could protect the host cells from the DC-induced cytotoxicity by reducing alkoxyl radical generation. The DC-induced cytotoxicity among the four metal electrodes was varied (Fig.…”

Section: Discussionmentioning

confidence: 90%

“…The cytotoxic activity of the DC treatment against PMNs was assessed, with the cell counting kit as described previously (4,5). Briefly, 200 L of the cell suspensions (5 ϫ 10 5 cells/well) were transferred to the 96-microwell culture plate (Falcon 3072, Becton Dickinson) and incubated for 4 hours with the cell counting kit.…”

Section: Cytotoxic Assaymentioning

confidence: 99%

“…We have also shown that different types of free radicals were generated from metal electrodes as follows, alkoxyl radical (St, Ni-Ti electrode), hydrogen radical (Ag and Au electrode), and both carbon and alkoxyl radicals (Zn electrode), respectively (5). Both free radicals and metal ions are a potential cause of oxidative stress on various cells (6).…”

mentioning

confidence: 93%

“…Recently, it has been reported that iontophoresis increased the transdentinal delivery of several proteins that may inhibit invasive cervical resorption (3). We have previously shown that direct current (DC) with antibacterial agents used for iontophoresis-induced necrotic cell death (4) and the DC-induced cytotoxicity may be because of the generated free radicals and metal ions released from metal electrodes (5). These results may explain the reason of the side effects of iontophoresis.…”

mentioning

confidence: 99%

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Glutathione Can Efficiently Prevent Direct Current–induced Cytotoxicity

Nakamura

Shimetani

Fujii

et al. 2008

Journal of Endodontics

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Electrode Reactions of Elemental (White) Phosphorus and Phosphane PH₃

2013

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Electrochemical methods have become important from the viewpoint of the development and creation of new ecologically safe and resource‐saving chemical processes. The use of electrons as a “universal” and “inexhaustible” reagent for chemical reactions is the main advantage of electrochemical tools. Although electrochemical processes have been already successfully applied in the preparation of different classes of practically important chemical compounds, a very limited number of industrially applicable electrochemical processes that involve elemental (white) phosphorus is currently known, except for some examples of the electrochemical production of phosphane PH3. These are mainly associated with the investigation of bulk reactions of the electrochemically activated P4 molecule and phosphorus intermediates derived therefrom; very limited attention has been paid to the electrode surface reactions as a key stage of the electrochemical process. In this Microreview we describe the electrode reactions of elemental (white) phosphorus and phosphane that accompany the electrochemical process of the preparation of some phosphorus compounds starting directly from P4. The important role of the nature of the electrode material, the reactivity of the formed phosphorus intermediates and the surface processes are discussed. Moreover, recently, we succeeded in discovering a new, previously unstable phosphorus compound, phosphane oxide H3PO, which can be easily produced using an electrochemical method by mild anodic oxidation of phosphane PH3 electrochemically formed from P4. In this respect, some attention has been paid to the electrochemically induced processes of PH3 oxidation that result in the selective formation of phosphane oxide, which is of high interest from the viewpoint of the investigation of its properties and reactivity.

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Role of Free Radicals and Metal Ions in Direct Current-Induced Cytotoxicity

Cited by 6 publications

References 21 publications

Isolation and Anti-Inflammatory Activity Evaluation of Triterpenoids and a Monoterpenoid Glycoside fromHarpagophytum procumbens

Isolation and Anti-Inflammatory Activity Evaluation of Triterpenoids and a Monoterpenoid Glycoside fromHarpagophytum procumbens

Glutathione Can Efficiently Prevent Direct Current–induced Cytotoxicity

Electrode Reactions of Elemental (White) Phosphorus and Phosphane PH₃

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Role of Free Radicals and Metal Ions in Direct Current-Induced Cytotoxicity

Cited by 6 publications

References 21 publications

Isolation and Anti-Inflammatory Activity Evaluation of Triterpenoids and a Monoterpenoid Glycoside fromHarpagophytum procumbens

Isolation and Anti-Inflammatory Activity Evaluation of Triterpenoids and a Monoterpenoid Glycoside fromHarpagophytum procumbens

Glutathione Can Efficiently Prevent Direct Current–induced Cytotoxicity

Electrode Reactions of Elemental (White) Phosphorus and Phosphane PH3

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Product

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About

Electrode Reactions of Elemental (White) Phosphorus and Phosphane PH₃