Role of Forward Translocation in Nucleoside Triphosphate Phosphohydrolase I (NPH I)-mediated Transcription Termination of Vaccinia Virus Early Genes

Tate, Jessica; Gollnick, Paul

doi:10.1074/jbc.m111.263822

Cited by 4 publications

(3 citation statements)

References 52 publications

(85 reference statements)

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“…It has previously been demonstrated that both Rap94 and VTF/CE are involved in the recognition of the termination motif, which may pause the elongating polymerase (Christen et al, 2008;Luo et al, 1995;Tate and Gollnick, 2015). NPH-I may then cause transcript extrusion from the active site by a 5 0 to 3 0 translocase activity on the non-template strand (Hindman and Gollnick, 2016;Tate and Gollnick, 2011). Provided the observed location of the CEC near the putative RNA exit tunnel is relevant for a termination intermediate, CEC may be involved in the recognition of the termination signal.…”

Section: Discussionmentioning

confidence: 99%

Structural Basis of Poxvirus Transcription: Vaccinia RNA Polymerase Complexes

Grimm

Hillen

Bedenk

et al. 2019

Cell

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Section: Discussionmentioning

confidence: 99%

Structural Basis of Poxvirus Transcription: Vaccinia RNA Polymerase Complexes

Grimm

Hillen

Bedenk

et al. 2019

Cell

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“…The purified virions were quantified by optical density at 260 nm (1 OD 260 = 64 μg virus). Extracts for early gene transcription reactions were prepared from purified virions as described previously (Tate & Gollnick, 2011). …”

Section: Methodsmentioning

confidence: 99%

“…Termination itself is catalyzed by a DNA-dependent nucleoside triphosphate phosphohydrolase (NPHI, gene D11), which interacts with the early form of the RNA polymerase through contacts with the early gene specific H4 subunit (Deng & Shuman, 1996; Deng & Shuman, 1998; Christen et al, 1998; Mohamed & Niles, 2000). CE can induce pausing by elongating RNA polymerase, which could theoretically provide a properly configured ternary complex of polymerase, DNA and RNA, primed for NPHI termination activity (Tate & Gollnick, 2011). The mRNA capping activity of CE is not required for early gene transcription termination, however both the D1 and D12 subunits must be present (Luo et al, 1995; Condit et al, 1996; Yu & Shuman, 1996).…”

Section: Introductionmentioning

confidence: 99%

Biochemical analysis of the multifunctional vaccinia mRNA capping enzyme encoded by a temperature sensitive virus mutant

et al. 2016

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Prior biochemical analysis of the heterodimeric vaccinia virus mRNA capping enzyme suggests roles not only in mRNA capping but also in early viral gene transcription termination and intermediate viral gene transcription initiation. Prior phenotypic characterization of Dts36, a temperature sensitive virus mutant affecting the large subunit of the capping enzyme was consistent with the multifunctional roles of the capping enzyme in vivo. We report a biochemical analysis of the capping enzyme encoded by Dts36. Of the three enzymatic activities required for mRNA capping, the guanylyltransferase and methyltransferase activities are compromised while the triphosphatase activity and the D12 subunit interaction are unaffected. The mutant enzyme is also defective in stimulating early gene transcription termination and intermediate gene transcription initiation in vitro. These results confirm that the vaccinia virus mRNA capping enzyme functions not only in mRNA capping but also early gene transcription termination and intermediate gene transcription initiation in vivo.

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The role of vaccinia termination factor and cis-acting elements in vaccinia virus early gene transcription termination

Tate

Gollnick

2015

Virology

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Vaccinia virus early gene transcription termination requires the virion form of the viral RNA polymerase (vRNAP), Nucleoside Triphosphate Phosphohydrolase I (NPHI), ATP, the vaccinia termination factor (VTF), and a U5NU termination signal in the nascent transcript. VTF, also the viral mRNA capping enzyme, binds U5NU, and NPHI hydrolyzes ATP to release the transcript. NPHI can release transcripts independent of VTF and U5NU if vRNAP is not actively elongating. However, VTF and U5NU are required for transcript release from an elongating vRNAP, suggesting that the function of VTF and U5NU may be to stall the polymerase. Here we demonstrate that VTF inhibits transcription elongation by enhancing vRNAP pausing. Hence VTF provides the connection between the termination signal in the RNA transcript and viral RNA polymerase to initiate transcription termination. We also provide evidence that a second cis-acting element downstream of U5NU influences the location and efficiency of early gene transcription termination.

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Role of Forward Translocation in Nucleoside Triphosphate Phosphohydrolase I (NPH I)-mediated Transcription Termination of Vaccinia Virus Early Genes

Cited by 4 publications

References 52 publications

Structural Basis of Poxvirus Transcription: Vaccinia RNA Polymerase Complexes

Structural Basis of Poxvirus Transcription: Vaccinia RNA Polymerase Complexes

Biochemical analysis of the multifunctional vaccinia mRNA capping enzyme encoded by a temperature sensitive virus mutant

The role of vaccinia termination factor and cis-acting elements in vaccinia virus early gene transcription termination

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