Role of Follistatin in Promoting Adipogenesis in Women

Flanagan, John N.; Linder, Kristina; Mejhert, Niklas; Dungner, Elisabeth; Wåhlén, Kerstin; Decaunes, Pauline; Rydén, Mikael; Björklund, Peyman; Arver, Stefan; Bhasin, Shalender; Bouloumié, Anne; Dahlman, Ingrid

doi:10.1210/jc.2008-2005

Cited by 55 publications

(49 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Notably, this human study did not evaluate follistatin expression in adipose tissues which is one tissue in which follistatin gene was expressed in the current study. More recently, follistatin gene expression has been detected in human adipose tissues with greater expression noted in subcutaneous as opposed to visceral adipose depots [52]. These data agree with the findings of the current study where follistatin expression was notably minimal in omental and mesenteric depots, in marked contrast to the clear expression noted in the other adipose tissue depots studied.…”

Section: Discussionsupporting

confidence: 92%

Post-Mortem Stability of RNA in Skeletal Muscle and Adipose Tissue and the Tissue-Specific Expression of Myostatin, Perilipin and Associated Factors in the Horse

Morrison

Chen

Harris³

et al. 2014

PLoS ONE

View full text Add to dashboard Cite

Obesity, a major concern for equine welfare, is highly prevalent in the leisure horse population. Skeletal-muscle and adipose tissues are important determinants of maintenance energy requirements. The myostatin and perilipin pathways play key roles in the regulation of muscle mass and lipolysis respectively and have both been associated with obesity predisposition in other mammalian species. High quality samples, suitable for molecular biology, are an essential prerequisite for detailed investigations of gene and protein expression. Hence, this study has evaluated a) the post-mortem stability of RNA extracted from skeletal-muscle and adipose-tissues collected under commercial conditions and b) the tissue-specific presence of myostatin, the moystatin receptor (activin receptor IIB, ActRIIB), follistatin and perilipin, genes and proteins across a range of equine tissues. Objectives were addressed using tissues from 7 Thoroughbred horses presented for slaughter at a commercial abattoir; a) samples were collected at 7 time-points from Masseter muscle and perirenal adipose from 5 minutes to 6 hours post-mortem. Extracted RN was appraised by Optical Density analysis and agarose-gel electrophoresis. b) Quantitative real time PCR and Western Blotting were used to evaluate gene and protein expression in anatomically-defined samples collected from 17 tissues (6 organs, 4 skeletal muscles and 7 discrete adipose depots). The results indicate that, under the present collection conditions, intact, good quality RNA could be extracted from skeletal-muscle for up to 2 hours post-mortem. However, RNA from adipose tissue may be more susceptible to degradation/contamination and samples should be collected no later than 30 minutes post-mortem. The data also show that myostatin and ActRIIB genes and proteins were almost exclusively expressed in skeletal muscle. The follistatin gene showed a more diverse gene expression profile, with expression evident in several organs, adipose tissue depots and skeletal muscles. Perilipin gene and protein were almost exclusively expressed by adipose tissue.

show abstract

Section: Discussionsupporting

confidence: 92%

Post-Mortem Stability of RNA in Skeletal Muscle and Adipose Tissue and the Tissue-Specific Expression of Myostatin, Perilipin and Associated Factors in the Horse

Morrison

Chen

Harris³

et al. 2014

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…However, EE-CA and PioFluMet had divergent effects on markers such as circulating CRP, leptin, HMW adiponectin, cholesterol, and triglycerides, and on carotid IMT, lean mass, and visceral hepatic adiposity, all these divergences favoring treatment with PioFluMet. The circulating levels of follistatin, a gly- coprotein that promotes adipogenesis in women (20), were elevated on EE-CA but normal on PioFluMet. Safety markers such as ALT and AST evolved also in a direction that was more reassuring for PioFluMet.…”

Section: Discussionmentioning

confidence: 99%

Treatment of Androgen Excess in Adolescent Girls: Ethinylestradiol-Cyproteroneacetate Versus Low-Dose Pioglitazone-Flutamide-Metformin

Ibáñez

Díaz

Sebastiani

et al. 2011

The Journal of Clinical Endocrinology &Amp; Metabolism

View full text Add to dashboard Cite

show abstract

“…Thus, skeletal muscle does not seem to secrete follistatin to any significant extent. In cultured white adipose tissue, follistatin is secreted into the media, suggesting a potential contribution to systemic levels (35). However, while follistatin gene expression is markedly doi: 10.1210/jc.2015-3668 press.endocrine.org/journal/jcem 555 increased in the liver in response to exercise in mice (11), no increase was detected in tissues such as adipose tissue, muscle, heart, kidney, and spleen (11).…”

Section: Origin Of Circulating Follistatin In Manmentioning

confidence: 99%

Circulating Follistatin Is Liver-Derived and Regulated by the Glucagon-to-Insulin Ratio

Hansen

Rütti

Arous

et al. 2016

The Journal of Clinical Endocrinology & Metabolism

120

View full text Add to dashboard Cite

show abstract

Role of Follistatin in Promoting Adipogenesis in Women

Abstract: Follistatin is a new adipokine important for adipogenesis. Down-regulated WAT expression of follistatin in obesity may counteract adiposity but could, by inhibiting adipogenesis, contribute to hypertrophic obesity (large fat cells) and insulin resistance.

Cited by 55 publications

References 34 publications

Post-Mortem Stability of RNA in Skeletal Muscle and Adipose Tissue and the Tissue-Specific Expression of Myostatin, Perilipin and Associated Factors in the Horse

Post-Mortem Stability of RNA in Skeletal Muscle and Adipose Tissue and the Tissue-Specific Expression of Myostatin, Perilipin and Associated Factors in the Horse

Treatment of Androgen Excess in Adolescent Girls: Ethinylestradiol-Cyproteroneacetate Versus Low-Dose Pioglitazone-Flutamide-Metformin

Circulating Follistatin Is Liver-Derived and Regulated by the Glucagon-to-Insulin Ratio

Contact Info

Product

Resources

About