Role of Fission Yeast Tup1-like Repressors and Prr1 Transcription Factor in Response to Salt Stress

Greenall, Amanda; Hadcroft, Andrew P.; Malakasi, Panagiota; Jones, Nic; Morgan, Brian A.; Hoffman, Charles S.; Whitehall, Simon K.

doi:10.1091/mbc.01-12-0568

Cited by 41 publications

(42 citation statements)

References 57 publications

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“…For example, heat-specific gene expression was independent of Sty1p, whereas H 2 O 2 -specific gene expression depended to a large part on Sty1p. The characterization of the cation-specific induction of cta3 in S. pombe illustrates how Sty1p may regulate stress-specific genes (Greenall et al, 2002). cta3 encodes a cation transporter, whose induction requires both activation of Sty1p and derepression of the Tup11/12p complex.…”

Section: Discussionmentioning

confidence: 99%

Global Transcriptional Responses of Fission Yeast to Environmental Stress

Chen

Toone

Mata

et al. 2003

MBoC

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727

988

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We explored transcriptional responses of the fission yeast Schizosaccharomyces pombe to various environmental stresses. DNA microarrays were used to characterize changes in expression profiles of all known and predicted genes in response to five stress conditions: oxidative stress caused by hydrogen peroxide, heavy metal stress caused by cadmium, heat shock caused by temperature increase to 39°C, osmotic stress caused by sorbitol, and DNA damage caused by the alkylating agent methylmethane sulfonate. We define a core environmental stress response (CESR) common to all, or most, stresses. There was a substantial overlap between CESR genes of fission yeast and the genes of budding yeast that are stereotypically regulated during stress. CESR genes were controlled primarily by the stress-activated mitogen-activated protein kinase Sty1p and the transcription factor Atf1p. S. pombe also activated gene expression programs more specialized for a given stress or a subset of stresses. In general, these "stress-specific" responses were less dependent on the Sty1p mitogen-activated protein kinase pathway and may involve specific regulatory factors. Promoter motifs associated with some of the groups of coregulated genes were identified. We compare and contrast global regulation of stress genes in fission and budding yeasts and discuss evolutionary implications.

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Section: Discussionmentioning

confidence: 99%

Global Transcriptional Responses of Fission Yeast to Environmental Stress

Chen

Toone

Mata

et al. 2003

MBoC

Self Cite

727

988

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“…In addition to its Pap1-supporting role (Chen et al, 2008;Calvo et al, 2012), Prr1 induces stress genes independently of Sty1 (Ohmiya et al, 1999(Ohmiya et al, , 2000 and it supports the induction of Atf1-dependent genes (Calvo et al, 2012;Greenall et al, 2002). No orthologous protein of Prr1 exists in humans (Penkett et al, 2006), while one of the Prr1 orthologs in budding yeast, SKN7, assists the YAP1 TF (i.e.…”

Section: Different Fission Yeast Responses To Oxidative Stress Of Difmentioning

confidence: 99%

Oxidative stress response pathways: Fission yeast as archetype

Papadakis¹,

Workman²

2015

Critical Reviews in Microbiology

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Schizosaccharomyces pombe is a popular model eukaryotic organism to study diverse aspects of mammalian biology, including responses to cellular stress triggered by redox imbalances within its compartments. The review considers the current knowledge on the signaling pathways that govern the transcriptional response of fission yeast cells to elevated levels of hydrogen peroxide. Particular attention is paid to the mechanisms that yeast cells employ to promote cell survival in conditions of intermediate and acute oxidative stress. The role of the Sty1/Spc1/Phh1 mitogen-activated protein kinase in regulating gene expression at multiple levels is discussed in detail.

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“…Tup1 represses the expression of genes via distinct mechanisms: by establishing a repressive chromatin structure around the target gene promoter, by recruiting histone deacetylases, and by directly interfering with the general transcription machinery (14)(15)(16)(17)(18). Two Tup1 orthologs in Schizosaccharomyces pombe, Tup11 and Tup12 (Tup11/12), regulate multiple stress-responsive genes, including the fbp1 ϩ and cta3 ϩ genes, to provide stress specificity (19,20). However, the precise molecular mechanisms of Tup1 family proteins in gene repression have not been fully uncovered.…”

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confidence: 99%

Antagonistic Controls of Chromatin and mRNA Start Site Selection by Tup Family Corepressors and the CCAAT-Binding Factor

Asada

Takemata

Hoffman

et al. 2015

Molecular and Cellular Biology

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The Tup family corepressors contribute to critical cellular responses, such as the stress response and differentiation, presumably by inducing repressive chromatin, though the precise repression mechanism remains to be elucidated. E ukaryotic chromosomal DNA is packaged in a highly organized and condensed chromatin structure. Many DNA-associated reactions, including DNA damage repair, replication, recombination, and transcription, are regulated by the chromatin structure (1, 2). The chromatin structure is modulated by two distinct classes of regulators, histone modification enzymes and ATP-dependent chromatin remodeling factors (3, 4). Such regulatory components are recruited by two types of cis-acting regulatory factors, transcriptional activators and repressors. Transcriptional activators and repressors bind to cis-acting elements to activate and repress transcription, respectively, by affecting the chromatin structure and regulating RNA polymerase II accumulation in the promoter region (5-7). These transcriptional regulators also interact with coactivators and corepressors to regulate gene expression (8, 9). The Tup family transcriptional corepressors are conserved between yeast and humans and regulate gene expression during the stress response and cellular differentiation (10, 11). Saccharomyces cerevisiae Tup1 represses some genes regulated by cell type, glucose, oxidative stress, DNA damage, and other cellular stress responses (12, 13). Tup1 represses the expression of genes via distinct mechanisms: by establishing a repressive chromatin structure around the target gene promoter, by recruiting histone deacetylases, and by directly interfering with the general transcription machinery (14-18). Two Tup1 orthologs in Schizosaccharomyces pombe, Tup11 and Tup12 (Tup11/12), regulate multiple stress-responsive genes, including the fbp1 ϩ and cta3 ϩ genes, to provide stress specificity (19,20). However, the precise molecular mechanisms of Tup1 family proteins in gene repression have not been fully uncovered.The fbp1 ϩ gene encodes fructose-1,6-bisphosphatase and is robustly induced upon glucose starvation (21,22). fbp1 ϩ expression is strictly repressed by Tup11/12 and activated by the transcriptional activators Atf1, Rst2, and Php5 (23-26). Atf1, a bZIP protein, is regulated through phosphorylation by the mitogen-activated protein kinase pathway (27-29), while Rst2, a C 2 H 2 Zn finger-type protein, is under the regulation of the protein kinase A pathway (23, 30). Php5, a component of the S. pombe CCAATbinding factor (CBF; also known as NF-Y) that possesses a histone hold domain, forms a complex with Php2/Php3 and contributes to cyc1 ϩ transcription (31, 32). In addition, two cis-acting elements required for fbp1 ϩ transcription have been identified (33). Upstream activation sequence 1 (UAS1) contains a cyclic AMP response element (CRE) and is the binding site for Atf1 and its binding partner, Pcr1 (34), while UAS2 resembles the S. cerevisiae stress response element (STRE) and serves as the binding site for Rst2 (...

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Role of Fission Yeast Tup1-like Repressors and Prr1 Transcription Factor in Response to Salt Stress

Cited by 41 publications

References 57 publications

Global Transcriptional Responses of Fission Yeast to Environmental Stress

Global Transcriptional Responses of Fission Yeast to Environmental Stress

Oxidative stress response pathways: Fission yeast as archetype

Antagonistic Controls of Chromatin and mRNA Start Site Selection by Tup Family Corepressors and the CCAAT-Binding Factor

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