Role of fibronectin in the migration of fibroblasts into plasma clots.

Knox, P. P.; Crooks, Sheila; Rimmer, Caroline S.

doi:10.1083/jcb.102.6.2318

Cited by 116 publications

(70 citation statements)

References 34 publications

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“…FXIII-mediated fibrin-fibrin complexes are unlikely, as certain cell types such as fibroblasts neither attach nor spread on cross-linked fibrin clots and they are not able to invade in fibronectin-depleted plasma clots. 43,44 In contrast, FXIII-mediated fibronectin-fibrin matrices have the ability to support adhesion and spreading of fibroblasts in vitro 45,46 . Thus, FXIIImediated fibrin-fibronectin matrices are the most likely to play a pivotal role as a survival factor during adult tissue remodeling.…”

Section: Discussionmentioning

confidence: 99%

Critical Role of Factor XIII in the Initial Stages of Carbon Tetrachloride–Induced Adult Liver Remodeling

Tsujimoto

Moriya

Sakai

et al. 2011

The American Journal of Pathology

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The transglutaminase-mediated, covalent cross-linking of proteins is an essential step in tissue remodeling after injury. This process provides tissues with extra rigidity and resistance against proteolytic degradation. Plasma coagulation factor XIII (FXIII) is a transglutaminase that promotes cross-linking of the extracellular matrix (ECM) components fibrin and fibronectin to form a provisional matrix in response to tissue damage. However, the functional requirement for this FXIII-mediated cross-linked provisional matrix in adult tissue remodeling remains to be defined. Although it has been proposed that the formation FXIII-mediated fibrin-fibronectin provisional matrix is a critical step for ECM remodeling, we show in an FXIII subunit A-deficient murine model of acute liver injury that the lack of FXIII subunit A did not interfere with collagen reconstruction and resolution after liver injury. Furthermore, FXIIIA deficiency caused significantly increased hepatocyte apoptosis and a delay in hepatocyte regeneration after injury, which were accompanied by a significantly high induction of p53 expression. These findings suggest novel functions of FXIII that the FXIII-mediated covalently crosslinked matrix could promote survival signals for hepatocytes in adult tissue remodeling. Covalent cross-linking between proteins, including in the provisional matrix, is catalyzed by transglutaminases (TGs). This is an important process for tissue remodeling as it generates extra rigidity and a resistance against proteolytic degradation. 1 Plasma coagulation factor Xlll (FXlll), one of the TG family members, circulates as a heterotetramer composed of two catalytic A subunits (FXIII A) and two noncatalytic B subunits (FXIII B) (A 2 B 2 ), which are activated by thrombinmediated cleavage. FXlll is a Ca 2ϩ -dependent TG and catalyzes the formation of -(␥-glutamyl) lysyl crosslinks between specific protein pairs. The cross-linking of fibrin molecules by FXIII to form ␥-chain dimerization is the final step of blood coagulation. 2-4 FXIII also acts on fibronectin-fibronectin and fibrin-fibronectin cross-linking. 5,6 Thus, the action of FXIII in forming covalent crosslinkages clearly plays a vital role in establishing the mechanical stability of the clot, which is critical for homeostatic functions. 2,4 Growing evidence demonstrates that the source of FXIII is not only from plasma: Megakaryocytes/ platelets and monocytes/macrophages also contain cellular FXIII subunit A in their cytoplasm. 7,8 Acute liver injury mediated by the hepatitis virus or hepatotoxins causes massive hepatocyte apoptosis and/or fatal liver damage, and eventually liver transplantation is required. 9,10 Identification of hepatic survival factors is critical for successful therapeutic intervention in liver failure. After acute injury, the liver undergoes a wound-healing response to restore tissue architecture and maintain homeostasis. 11 It requires both a well-orchestrated proliferation of cells and the reconstruction of the extracellular matrix (ECM). A central...

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Section: Discussionmentioning

confidence: 99%

Critical Role of Factor XIII in the Initial Stages of Carbon Tetrachloride–Induced Adult Liver Remodeling

Tsujimoto

Moriya

Sakai

et al. 2011

The American Journal of Pathology

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“…Its expression is highly upregulated after wounding and, together with fibrin, it acts as a provisional matrix and promotes, e.g., keratinocyte and fibroblast migration (Kim et al, 1992;Knox et al, 1986;Clark et al, 1982;Postlethwaite et al, 1981).…”

Section: Histocbem Cytochem 44:1311-1322 19%)mentioning

confidence: 99%

Extracellular matrix characterization during healing of full-thickness wounds treated with a collagen/elastin dermal substitute shows improved skin regeneration in pigs.

Lamme¹,

Vries²,

Veen³

et al. 1996

J Histochem Cytochem.

134

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We investigated the architecture of the extracellular matrix (ECM) during healing of full-thickness wounds in the pig. Two different treatments, one based on epidermal transplantation (split skin mesh grafts, SP wounds) and one consisting of a combination of epidermal transplantation and a dermal matrix substitute (MA wounds) were compared. The dermal matrix consisted of native bovine collagen coated with elastin hydrolysate. The latter treatment r e d u d wound contraction and improved tissue regeneration. The expression patterns of fibronectin, von Willebrand factor, laminin, chondroitin sulfate, and elastin, detected by immunohistochemistry, were examined in time and indicated different stages of healing. During the early phase of healing the dermal matrix induced more granulation tissue, a *rent fibronectin expression pattem, and rapid vascular cell ingrowth (von Willebrand factor). Furthermore, in the MA wounds chondroitin sulfate was detected earlier in the basement membrane and fibronectin staining disappeared more

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“…basement membranes and it is also present in blood plasma, as a disulphide linked dimer. It is important for cell anchorage, cell motility [1,2] and for normal wound healing [3]. FN contains discrete domains with binding sites for fibrin, collagen, DNA, cell surfaces and heparin.…”

Section: Introductionmentioning

confidence: 99%

Donor and acceptor splice signals within an exon of the human fibronectin gene: a new type of differential splicing

1986

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We have sequenccd that area of a human fibronectin gene clone which codes for a connecting strand separating the last two areas of the type lII homology. The gene has a complex exon with two 'AG' acceptor sites. One of these can be used (exon subdivision). In addition 93 basepairs inside the exon are sometimes spliced out as an intron. This is the third differential splicing found in the fibronectin gene transcript and it represcnts a new type of differential splicing. f)bronectin gene clone Differential splicing Nonhomologous connective strand

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Role of fibronectin in the migration of fibroblasts into plasma clots.

Cited by 116 publications

References 34 publications

Critical Role of Factor XIII in the Initial Stages of Carbon Tetrachloride–Induced Adult Liver Remodeling

Critical Role of Factor XIII in the Initial Stages of Carbon Tetrachloride–Induced Adult Liver Remodeling

Extracellular matrix characterization during healing of full-thickness wounds treated with a collagen/elastin dermal substitute shows improved skin regeneration in pigs.

Donor and acceptor splice signals within an exon of the human fibronectin gene: a new type of differential splicing

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