Role of fibroblast plasticity and heterogeneity in modulating angiogenesis and healing in the diabetic foot ulcer

Rai, Vikrant; Moellmer, Rebecca; Agrawal, Devendra K.

doi:10.1007/s11033-022-08107-4

Cited by 13 publications

(11 citation statements)

References 113 publications

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“…Furthermore, starting from the same density of plated cells, and observing the cultures after 6 and 24 h, cells are differently distributed in the well. Indeed, a reduction in the growth rate of ulcer-HDFs after only 6 h was observed, in accordance with the literature data [25,26]. After 24 h, differences in concentration, density, size, arrangement and orientation of ulcer-HDFs were still detectable with respect to normal-HDFs (Figure 1).…”

Section: Cell Morphologysupporting

confidence: 91%

Pulsed Radiofrequency Electromagnetic Fields as Modulators of Inflammation and Wound Healing in Primary Dermal Fibroblasts of Ulcers

Costantini,

Aielli,

Gualdi

et al. 2024

Bioengineering

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Venous leg ulcers are one of the most common nonhealing conditions and represent an important clinical problem. The application of pulsed radiofrequency electromagnetic fields (PRF-EMFs), already applied for pain, inflammation, and new tissue formation, can represent a promising approach for venous leg ulcer amelioration. This study aims to evaluate the effect of PRF-EMF exposure on the inflammatory, antioxidant, cell proliferation, and wound healing characteristics of human primary dermal fibroblasts collected from venous leg ulcer patients. The cells’ proliferative and migratory abilities were evaluated by means of a BrdU assay and scratch assay, respectively. The inflammatory response was investigated through TNFα, TGFβ, COX2, IL6, and IL1β gene expression analysis and PGE2 and IL1β production, while the antioxidant activity was tested by measuring GSH, GSSG, tGSH, and GR levels. This study emphasizes the ability of PRF-EMFs to modulate the TGFβ, COX2, IL6, IL1β, and TNFα gene expression in exposed ulcers. Moreover, it confirms the improvement of the proliferative index and wound healing ability presented by PRF-EMFs. In conclusion, exposure to PRF-EMFs can represent a strategy to help tissue repair, regulating mediators involved in the wound healing process.

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Section: Cell Morphologysupporting

confidence: 91%

Pulsed Radiofrequency Electromagnetic Fields as Modulators of Inflammation and Wound Healing in Primary Dermal Fibroblasts of Ulcers

Costantini,

Aielli,

Gualdi

et al. 2024

Bioengineering

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“…16 HBOT is thought to increase local tissue perfusion, which stimulates collagen synthesis, the generation of growth factors, and neovascularization, and reduces oxidative stress, hence improving wound healing. 70,71 Additionally, HBOT creates a bactericidal action against anaerobic microorganisms, hence minimizing the need for antibacterial medications. 72 It's still debatable if HBOT has therapeutic significance, as determined by clinical research.…”

Section: Gradementioning

confidence: 99%

Mesenchymal stem cell therapy in diabetic foot ulcer: An updated comprehensive review

Hetta,

Elsaghir,

Sijercic

et al. 2024

Health Science Reports

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BackgroundDiabetes has evolved into a worldwide public health issue. One of the most serious complications of diabetes is diabetic foot ulcer (DFU), which frequently creates a significant financial strain on patients and lowers their quality of life. Up until now, there has been no curative therapy for DFU, only symptomatic relief or an interruption in the disease's progression. Recent studies have focused attention on mesenchymal stem cells (MSCs), which provide innovative and potential treatment candidates for several illnesses as they can differentiate into various cell types. They are mostly extracted from the placenta, adipose tissue, umbilical cord (UC), and bone marrow (BM). Regardless of their origin, they show comparable features and small deviations. Our goal is to investigate MSCs' therapeutic effects, application obstacles, and patient benefit strategies for DFU therapy.MethodologyA comprehensive search was conducted using specific keywords relating to DFU, MSCs, and connected topics in the databases of Medline, Scopus, Web of Science, and PubMed. The main focus of the selection criteria was on English‐language literature that explored the relationship between DFU, MSCs, and related factors.Results and DiscussionNumerous studies are being conducted and have demonstrated that MSCs can induce re‐epithelialization and angiogenesis, decrease inflammation, contribute to immunological modulation, and subsequently promote DFU healing, making them a promising approach to treating DFU. This review article provides a general snapshot of DFU (including clinical presentation, risk factors and etiopathogenesis, and conventional treatment) and discusses the clinical progress of MSCs in the management of DFU, taking into consideration the side effects and challenges during the application of MSCs and how to overcome these challenges to achieve maximum benefits.ConclusionThe incorporation of MSCs in the management of DFU highlights their potential as a feasible therapeutic strategy. Establishing a comprehensive understanding of the complex relationship between DFU pathophysiology, MSC therapies, and related obstacles is essential for optimizing therapy outcomes and maximizing patient benefits.

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“…In normal wounds, damaged and contracted blood vessels result in wound hypoxia, keratinocyte migration, early angiogenesis, proliferation and clonal expansion of fibroblast, transcription and synthesis of key growth factors, as well as cytokines. Subsequently, fibroblasts aggregate at the injured site for the participation of the formation of early granulation tissue and the activation of the contraction process 45,46 . In DFU, fibroblasts may be aging due to the phenotypic changes, which leads to their weakened proliferative response to growth factors and then affects granulation tissue formation and wound healing 47–49 .…”

Section: Pathogenesis Of Dfumentioning

confidence: 99%

“…Subsequently, fibroblasts aggregate at the injured site for the participation of the formation of early granulation tissue and the activation of the contraction process. 45 , 46 In DFU, fibroblasts may be aging due to the phenotypic changes, which leads to their weakened proliferative response to growth factors and then affects granulation tissue formation and wound healing. 47 , 48 , 49 Due to hyperglycemia, the original healing process is impaired and the biological functions of many cells have changed.…”

Section: Pathogenesis Of Dfumentioning

confidence: 99%

Advanced multifunctional hydrogels for diabetic foot ulcer healing: Active substances and biological functions

Li,

Leng,

Liu

et al. 2024

Journal of Diabetes

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AimHydrogels with excellent biocompatibility and biodegradability can be used as the desirable dressings for the therapy of diabetic foot ulcer (DFU). This review aimed to summarize the biological functions of hydrogels, combining with the pathogenesis of DFU.MethodsThe studies in the last 10 years were searched and summarized from the online database PubMed using a combination of keywords such as hydrogel and diabetes. The biological functions of hydrogels and their healing mechanism on DFU were elaborated.ResultsIn this review, hydrogels were classified by their active substances such as drugs, cytokines, photosensitizers, and biomimetic peptide. Based on this, the biological functions of hydrogels were summarized by associating the pathogenesis of DFU, including oxidative stress, chronic inflammation, cell phenotype change, vasculopathy, and infection. This review also pointed out some of the shortcomings of hydrogels in present researches.ConclusionsHydrogels were classified into carrier hydrogels and self‐functioning hydrogels in this review. Besides, the functions and components of existing hydrogels were clarified to provide assistance for future researches and clinical applications.image

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Role of fibroblast plasticity and heterogeneity in modulating angiogenesis and healing in the diabetic foot ulcer

Cited by 13 publications

References 113 publications

Pulsed Radiofrequency Electromagnetic Fields as Modulators of Inflammation and Wound Healing in Primary Dermal Fibroblasts of Ulcers

Pulsed Radiofrequency Electromagnetic Fields as Modulators of Inflammation and Wound Healing in Primary Dermal Fibroblasts of Ulcers

Mesenchymal stem cell therapy in diabetic foot ulcer: An updated comprehensive review

Advanced multifunctional hydrogels for diabetic foot ulcer healing: Active substances and biological functions

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