Role of Extracellular Transaldolase from Bifidobacterium bifidum in Mucin Adhesion and Aggregation

González-Rodríguez, Irene; Sánchez, Borja; Ruíz, Lorena; Turroni, Francesca; Ventura, Marco; Ruas‐Madiedo, Patricia; Gueimonde, Miguel; Margollés, Abelardo

doi:10.1128/aem.08024-11

Cited by 106 publications

(83 citation statements)

References 54 publications

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“…Specifically, we studied the impact of B. bifidum Bb on the intestinal microbial ecology of healthy adults. Strain Bb was isolated from the feces from a healthy adult woman and, according to in silico analysis of its draft genome (our unpublished data), possesses the genetic determinants known to support the B. bifidum-host interaction, including genes encoding sortase-dependent pili (12), mucin-metabolizing enzymes (23), human-milk oligosaccharide hydrolases (24), BopA outer surface lipoprotein (10), and Tal transaldolase (25). In this study, we used capsules containing approximately one billion viable Bb cells, which corresponds to the minimal daily dosage recommended for probiotics by the Italian Ministry of Health (26).…”

Section: Discussionmentioning

confidence: 99%

Consumption of a Bifidobacterium bifidum Strain for 4 Weeks Modulates Dominant Intestinal Bacterial Taxa and Fecal Butyrate in Healthy Adults

Gargari

Taverniti

Balzaretti

et al. 2016

Appl Environ Microbiol

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Modulation of the intestinal microbial ecosystem (IME) is a useful target to establish probiotic efficacy in a healthy population. We conducted a randomized, double-blind, crossover, and placebo-controlled intervention study to determine the impact of Bifidobacterium bifidum strain Bb on the IME of adult healthy volunteers of both sexes. High-throughput 16S rRNA gene sequencing was used to characterize the fecal microbiota before and after 4 weeks of daily probiotic cell consumption. The intake of approximately one billion live B. bifidum cells affected the relative abundance of dominant taxa in the fecal microbiota and modulated fecal butyrate levels. Specifically, Prevotellaceae (P ‫؍‬ 0.041) and Prevotella (P ‫؍‬ 0.034) were significantly decreased, whereas Ruminococcaceae (P ‫؍‬ 0.039) and Rikenellaceae (P ‫؍‬ 0.010) were significantly increased. We also observed that the probiotic intervention modulated the fecal concentrations of butyrate in a manner dependent on the initial levels of short-chain fatty acids (SCFAs). In conclusion, our study demonstrates that a single daily administration of Bifidobacterium bifidum strain Bb can significantly modify the IME in healthy (not diseased) adults. These findings demonstrate the need to reassess the notion that probiotics do not influence the complex and stable IME of a healthy individual. IMPORTANCEFoods and supplements claimed to contain health-promoting probiotic microorganisms are everywhere these days and mainly intended for consumption by healthy people. However, it is still debated what actual effects probiotic products may have on the healthy population. In this study, we report the results of an intervention trial aimed at assessing the modifications induced in the intestinal microbial ecosystem of healthy adults from the consumption of a probiotic product. Our results demonstrate that the introduction of a probiotic product in the dietary habits of healthy people may significantly modify dominant taxa of the intestinal microbiota, resulting in the modulation of short-chain fatty acid concentrations in the gut. The overall changes witnessed in the probiotic intervention indicate a mechanism of microbiota modulation that could have potential effects on human health.

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Section: Discussionmentioning

confidence: 99%

Consumption of a Bifidobacterium bifidum Strain for 4 Weeks Modulates Dominant Intestinal Bacterial Taxa and Fecal Butyrate in Healthy Adults

Gargari

Taverniti

Balzaretti

et al. 2016

Appl Environ Microbiol

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“…However, the antiserum produced against His 6 -BopA failed to decrease the adhesion of B. bifidum to mucus, indicating that BopA is not the major adhesin mediating the bifidobacterial adherence to mucus either. Previously, both DSM20456 and MIMBb75 were found to adhere to immobilized, commercially available mucus, and the surface-exposed proteins have been suggested to be involved in the binding (10,39). Transaldolase was reported to mediate the autoaggregation of the bifidobacterial cells at acidic pH, which was linked to the mucusbinding capacity of B. bifidum (10).…”

Section: Discussionmentioning

confidence: 99%

“…Previously, both DSM20456 and MIMBb75 were found to adhere to immobilized, commercially available mucus, and the surface-exposed proteins have been suggested to be involved in the binding (10,39). Transaldolase was reported to mediate the autoaggregation of the bifidobacterial cells at acidic pH, which was linked to the mucusbinding capacity of B. bifidum (10). However, as the autoaggregating phenotype of B. bifidum is strictly dependent on acidic pH (39), and since in this study all binding experiments were performed at neutral pH and autoaggregation was not observed, autoaggregation cannot explain the strong adhesion of B. bifidum to mucus at neutral pH.…”

Section: Discussionmentioning

confidence: 99%

“…Bifidobacteria are commensal inhabitants of the human gastrointestinal tract, and they can constitute a considerable part of an individual's gut microbiota, especially in breast-fed infants (6,7). Many Bifidobacterium species and strains adhere strongly to human intestinal epithelial cells in vitro (8,9), but only a few adhesion molecules have been described thus far (9)(10)(11). The expression of moonlighting proteins on the cell surface of Bifidobacterium seems to be common, and under certain circumstances these proteins could facilitate colonization of the human gut (12)(13)(14).…”

mentioning

confidence: 99%

“…The expression of moonlighting proteins on the cell surface of Bifidobacterium seems to be common, and under certain circumstances these proteins could facilitate colonization of the human gut (12)(13)(14). Recently, moonlighting transaldolase was reported to play a role in the autoaggregation and adhesion of Bifidobacterium bifidum to mucin (10).…”

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confidence: 99%

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BopA Does Not Have a Major Role in the Adhesion of Bifidobacterium bifidum to Intestinal Epithelial Cells, Extracellular Matrix Proteins, and Mucus

Kainulainen

Reunanen

Hiippala

et al. 2013

Appl Environ Microbiol

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The ability of bifidobacteria to adhere to the intestine of the human host is considered to be important for efficient colonization and achieving probiotic effects. Bifidobacterium bifidum strains DSM20456 and MIMBb75 adhere well to the human intestinal cell lines Caco-2 and HT-29. The surface lipoprotein BopA was previously described to be involved in mediating adherence of B. bifidum to epithelial cells, but thioacylated, purified BopA inhibited the adhesion of B. bifidum to epithelial cells in competitive adhesion assays only at very high concentrations, indicating an unspecific effect. In this study, the role of BopA in the adhesion of B. bifidum was readdressed. The gene encoding BopA was cloned and expressed without its lipobox and hydrophobic signal peptide in Escherichia coli, and an antiserum against the recombinant BopA was produced. The antiserum was used to demonstrate the abundant localization of BopA on the cell surface of B. bifidum. However, blocking of B. bifidum BopA with specific antiserum did not reduce adhesion of bacteria to epithelial cell lines, arguing that BopA is not an adhesin. Also, adhesion of B. bifidum to human colonic mucin and fibronectin was found to be BopA independent. The recombinant BopA bound only moderately to human epithelial cells and colonic mucus, and it failed to bind to fibronectin. Thus, our results contrast the earlier findings on the major role of BopA in adhesion, indicating that the strong adhesion of B. bifidum to epithelial cell lines is BopA independent.

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Strain‐dependent effectivity, and protective role against enzymes of S‐layers in Lactiplantibacillus plantarum strains

Alp

2022

J Basic Microbiol

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The present study investigated whether the surface layer (S-layer), which is known to have a varying effect from strain to strain on aggregation, adhesion ability, also has an effect on the resistance of bacteria to digestive enzymes, phenol, lysozymes. The effect of S-layers on the resistance against various enzymes, aggregation and adhesion abilities, and strain specificity were determined of eight Lactiplantibacillus plantarum strains. Strains were treated with 5 M lithium chloride (LiCl) to extract the S-layers, the presence of this layer in those microorganisms was demonstrated by polyacrylamide gel electrophoresis. Scanning electron microscopy was used to visualize the separation of the S-layer, which surrounds the microorganism, from the microorganism by the LiCl. The images were taken three times, once at the beginning, once 30 min later, and once at the end of this process, which took 2 h in total. The effect against enzymes varied depending on the strain, but it was determined that all the tested strains had a serious loss of viability against phenol in the absence of an S-layer. Lpb. plantarum DA100 showed a maximum decrease against gastrointestinal system enzymes after the LiCl (96.48 ± 0.03% before and 66.46 ± 0.01% after LiCl). Lpb. plantarum DA255 showed a significant decrease against lysozyme (99.11 ± 0.00% before and 62.80 ± 0.0% after LiCl). Removal of the S-layer greatly affected the adhesion ability of some strains, while for others there was hardly any change. The results showed that the role of the S-layer may be strain-specific, the rate of effect can vary. The primary function of S-layer proteins is thought to contribute to the adhesion ability of bacteria. There are limited studies that have reported the protective property of this layer against various enzymes, however, our results showed that S-layer could be one of the resistance strategies developed by bacteria against enzymes.

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Role of Extracellular Transaldolase from Bifidobacterium bifidum in Mucin Adhesion and Aggregation

Cited by 106 publications

References 54 publications

Consumption of a Bifidobacterium bifidum Strain for 4 Weeks Modulates Dominant Intestinal Bacterial Taxa and Fecal Butyrate in Healthy Adults

Consumption of a Bifidobacterium bifidum Strain for 4 Weeks Modulates Dominant Intestinal Bacterial Taxa and Fecal Butyrate in Healthy Adults

BopA Does Not Have a Major Role in the Adhesion of Bifidobacterium bifidum to Intestinal Epithelial Cells, Extracellular Matrix Proteins, and Mucus

Strain‐dependent effectivity, and protective role against enzymes of S‐layers in Lactiplantibacillus plantarum strains

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