Role of Extracellular Matrix Signaling Cues in Modulating Cell Fate Commitment for Cardiovascular Tissue Engineering

Nakayama, Karina H.; Hou, Luqia; Huang, Ngan F.

doi:10.1002/adhm.201300620

Cited by 72 publications

(66 citation statements)

References 116 publications

(197 reference statements)

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“…Ideally, the synthetic biomaterials used in tissue engineering and cell delivery should create the same or similar microenvironment for the seeded cells as that in an ECM existing in vivo . However, native ECMs have very intricate structures and are composed of numerous types of proteins, many of which remain unidentified thus far [37,280]. Therefore, using conventional physicochemical methods, it is difficult to obtain a scaffold or substratum that has the same composition and the complex microstructure and architecture of an in vivo ECM [477].…”

Section: Future Directions and Outlookmentioning

confidence: 99%

Advancing biomaterials of human origin for tissue engineering

Chen

Liu

2016

Progress in Polymer Science

921

513

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Biomaterials have played an increasingly prominent role in the success of biomedical devices and in the development of tissue engineering, which seeks to unlock the regenerative potential innate to human tissues/organs in a state of deterioration and to restore or reestablish normal bodily function. Advances in our understanding of regenerative biomaterials and their roles in new tissue formation can potentially open a new frontier in the fast-growing field of regenerative medicine. Taking inspiration from the role and multi-component construction of native extracellular matrices (ECMs) for cell accommodation, the synthetic biomaterials produced today routinely incorporate biologically active components to define an artificial in vivo milieu with complex and dynamic interactions that foster and regulate stem cells, similar to the events occurring in a natural cellular microenvironment. The range and degree of biomaterial sophistication have also dramatically increased as more knowledge has accumulated through materials science, matrix biology and tissue engineering. However, achieving clinical translation and commercial success requires regenerative biomaterials to be not only efficacious and safe but also cost-effective and convenient for use and production. Utilizing biomaterials of human origin as building blocks for therapeutic purposes has provided a facilitated approach that closely mimics the critical aspects of natural tissue with regard to its physical and chemical properties for the orchestration of wound healing and tissue regeneration. In addition to directly using tissue transfers and transplants for repair, new applications of human-derived biomaterials are now focusing on the use of naturally occurring biomacromolecules, decellularized ECM scaffolds and autologous preparations rich in growth factors/non-expanded stem cells to either target acceleration/magnification of the body's own repair capacity or use nature's paradigms to create new tissues for restoration. In particular, there is increasing interest in separating ECMs into simplified functional domains and/or biopolymeric assemblies so that these components/constituents can be discretely exploited and manipulated for the production of bioscaffolds and new biomimetic biomaterials. Here, following an overview of tissue auto-/allo-transplantation, we discuss the recent trends and advances as well as the challenges and future directions in the evolution and application of human-derived biomaterials for reconstructive surgery and tissue engineering. In particular, we focus on an exploration of the structural, mechanical, biochemical and biological information present in native human tissue for bioengineering applications and to provide inspiration for the design of future biomaterials.

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Section: Future Directions and Outlookmentioning

confidence: 99%

Advancing biomaterials of human origin for tissue engineering

Chen

Liu

2016

Progress in Polymer Science

921

513

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“…It is well established that cell shape, cytoskeletal reorganization, and function are modulated by chemical and physical properties of an underlying substrate [39]. Future avenues of investigation include functional testing of the smooth muscle layer by pharmacologically induced vasoconstriction and elucidation of the cellular and molecular mechanisms underlying spatial nanopatterning-induced cellular effects.…”

Section: Resultsmentioning

confidence: 99%

Bilayered Vascular Graft Derived from Human Induced Pluripotent Stem Cells with Biomimetic Structure and Function

et al. 2015

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We developed an aligned bi-layered vascular graft derived from human induced pluripotent stem cells (iPSCs) that recapitulates the cellular composition, orientation, and anti-inflammatory function of blood vessels. The luminal layer consisted of longitudinal-aligned nanofibrillar collagen containing primary endothelial cells (ECs) or iPSC-derived ECs (iPSC-ECs). The outer layer contained circumferentially oriented nanofibrillar collagen with primary smooth muscle cells (SMCs) or iPSC-derived SMCs (iPSC-SMCs). On the aligned scaffolds, cells organized F-actin assembly within 8° from the direction of nanofibrils. When compared to randomly-oriented scaffolds, EC-seeded aligned scaffolds had significant reduced inflammatory response, based on adhesivity to monocytes. This study highlights the importance of anisotropic scaffolds in directing cell form and function, and has therapeutic significance as physiologically relevant blood vessels.

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“…Specifically this was due in part to adequate, yet more physiologically comparable fluid transfer, mechanical properties and both chemical and nanostructural contributions of the nHA. There was a slight decrease in collagen type I deposition on nHA scaffolds after 2 weeks of culture, but collagen type I content increased again after 3 weeks This may have been due to enhanced cellular migration on nHA conjugated scaffolds, as initial cellular migration and invasion has been shown to suppress both proliferation and tissue deposition, temporarily [41, 67–69]. …”

Section: Discussionmentioning

confidence: 99%

A synergistic approach to the design, fabrication and evaluation of 3D printed micro and nano featured scaffolds for vascularized bone tissue repair

et al. 2016

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3D bioprinting has begun to show great promise in advancing the development of functional tissue/organ replacements. However, to realize the true potential of 3D bioprinted tissues for clinical use requires the fabrication of an interconnected and effective vascular network. Solving this challenge is critical, as human tissue relies on an adequate network of blood vessels to transport oxygen, nutrients, other chemicals, biological factors and waste, in and out of the tissue. Here, we have successfully designed and printed a series of novel 3D bone scaffolds with both bone formation supporting structures and highly interconnected 3D microvascular mimicking channels, for efficient and enhanced osteogenic bone regeneration as well as vascular cell growth. Using a chemical functionalization process, we have conjugated our samples with nano hydroxyapatite (nHA), for the creation of novel micro and nano featured devices for vascularized bone growth. We evaluated our scaffolds with mechanical testing, hydrodynamic measurements and in vitro human mesenchymal stem cell (hMSC) adhesion (4 h), proliferation (1, 3 and 5 d) and osteogenic differentiation (1, 2 and 3 weeks). These tests confirmed bone-like physical properties and vascular-like flow profiles, as well as demonstrated enhanced hMSC adhesion, proliferation and osteogenic differentiation. Additional in vitro experiments with human umbilical vein endothelial cells also demonstrated improved vascular cell growth, migration and organization on micro-nano featured scaffolds.

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Role of Extracellular Matrix Signaling Cues in Modulating Cell Fate Commitment for Cardiovascular Tissue Engineering

Cited by 72 publications

References 116 publications

Advancing biomaterials of human origin for tissue engineering

Advancing biomaterials of human origin for tissue engineering

Bilayered Vascular Graft Derived from Human Induced Pluripotent Stem Cells with Biomimetic Structure and Function

A synergistic approach to the design, fabrication and evaluation of 3D printed micro and nano featured scaffolds for vascularized bone tissue repair

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