Role of exercise and ascorbate on plasma antioxidant capacity in thoroughbred race horses

White, Allan; Estrada, Manuel; Walker, Katharine; Wisnia, Pamela; Filgueira, Gonzalo; Valdés, Fernando; Araneda, Oscar F.; Behn, Claus; Martinez, R.

doi:10.1016/s1095-6433(00)00286-5

Cited by 100 publications

(85 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This result is in accord with other studies (22,48,50,52,62). These increases presumably reflect persistent damage to the muscles, resulting in a loss of cell membrane integrity, and consequent leakage of proteins into the blood.…”

Section: Figsupporting

confidence: 92%

“…Likewise, no difference was observed between the two marches, in spite the 2-week lapse between them (Table 2). This is in accord with other reports showing no change or even an increase in AA level (2,23,24,28,29,42,(47)(48)(49)(50). Other reports on intense but short-duration exercise have shown a marked decrease in AA and red blood cell glutathione levels (51).…”

Section: Figsupporting

confidence: 91%

See 1 more Smart Citation

Plasma antioxidant status and cell injury after severe physical exercise

Chevion

Moran

Heled

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

220

137

View full text Add to dashboard Cite

Strenuous exercise leads to an increase in metabolic rate, increased production of reactive oxygen species, and compromised antioxidant defense systems. To study the effects of oxidative stress during strenuous exercise, a homogeneous group of 31 male subjects participated in a 6-month, 5 days͞week training schedule involving two extreme marches of 50 km and 80 km at sea level, separated by 2 weeks of regular training. Each participant carried 35 kg of extra weight. Blood samples were drawn imediately before and after each march. Twenty-nine subjects completed the 50-km march, and only 16 completed the 80-km march. Plasma levels of reduced ascorbic acid, total ascorbate, and dehydroascorbate did not undergo significant changes during either march. However, the 50-and 80-km marches led to 25% and 37% increases, respectively, in plasma levels of uric acid; due presumably to increases in the metabolic rate and consequent pyrimidine nucleotide metabolism. Both marches led to Ϸ10-fold increase leakage of creatine phosphokinase into the plasma. Likewise, plasma levels of aspartate transaminase, a characteristic marker of liver injury, increased Ϸ4-fold. Plasma levels of bilirubin, creatine, urea, and glucose also increased. Plasma protein carbonyl content, a marker of protein oxidative damage, decreased significantly during each march. These results are discussed with respect to the consideration that elevation of the respiration rate during exercise leads to production of more reactive oxygen species than the antioxidant systems can scavenge. Plausible explanations for leakage of molecules into the plasma are discussed.

show abstract

Section: Figsupporting

confidence: 92%

Section: Figsupporting

confidence: 91%

Plasma antioxidant status and cell injury after severe physical exercise

Chevion

Moran

Heled

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

220

137

View full text Add to dashboard Cite

show abstract

“…Strenuous training on a daily basis may increase the rate of lipid peroxidation and protein oxidation, leading to an inflammatory reaction and muscle disease (Kinnunen et al 2005;Sacheck and Blumberg 2001). The protective role of antioxidant supplementation against excessive oxidative damage of trained horses was confirmed (Avellini et al 1999;White et al 2001;de Morffarts et al 2005).…”

Section: Horse Free Radicals Exercise Antioxidants Nutritionmentioning

confidence: 95%

Effect of Dietary Supplementation with Vitamin E and Selenium in Thoroughbred Horses on the Concentration of F2-isoprostanes in the Blood Plasma as a Marker of Lipid Peroxidation

et al. 2008

View full text Add to dashboard Cite

The objective of this study was to assess the effect of vitamin E and selenium (Se) supplementation on the plasma levels of F 2 -isoprostanes as a marker of oxidative stress in horses in their training period. Twelve healthy 3-year-old English thoroughbred horses were divided into two groups: control (n = 6) and experimental (n = 6). Feeding rations were adapted to a moderate workload. The horses of the experimental group received supplements of DL--tocopheryl acetate E (2 250 mg/day/horse) and of sodium selenite (0.5 mg/day/horse). The plasma concentrations of both antioxidants and F 2 -isoprostanes were monitored on days 0, 44 and 70. After 70 days of supplementation, the concentrations of selenium in the experimental group were significantly higher (P  0.05) compared to the beginning of the experiment (mean ± SE: 135. ). In the horses of the experimental group, plasma -tocopherol levels significantly increased from the 44 th day of supplementation compared to the beginning of the study as well to the control group (5.23 ± 0.52 mg·l -1 vs. 2.45 ± 0.25 mg·l -1 or 3.46 ± 0.34 mg·l -1 , respectively). The plasma concentration of F 2 -isoprostanes tended to be lower in the experimental group at the end than at the beginning of monitoring (156.8 ± 12.89 pg·ml -1 vs. 170.3 ± 60.8 pg·ml -1 ), although the control group showed the opposite trend (181.2 ± 15.67 pg·ml -1 vs. 137.0 ± 47.05 pg·ml -1 ). Nevertheless, none of these differences were significant because of the large variability of the individual values. It can be stated that supplementation of the diet used with selenium and vitamin E caused a non-significant decrease of F 2 -isoprostane concentration in the blood plasma only, and a significant increase of plasma concentrations of these antioxidants. The variation of isoprostane levels probably reflected rather the individual responses of the horses' organisms to the training workload.

show abstract

“…In equine medicine, several studies investigating the effect of different types of exercise (Avellini et al, 1995;Mills et al, 1996a;Chiaradia et al, 1998;Balogh et al, 2001;White et al, 2001;Kirschvink et al, 2002c;Marlin et al, 2002) as well as the effect of different thermal heat loads (Mills et al, 1996a;Hargreaves et al, 2002) are published. Despite some discrepancies between study results, which can be explained by different experimental designs, different analytic techniques and the use of different oxidant markers, it can be concluded that exercise is inducing significant alterations of the circulatory oxidant-antioxidant balance in horses.…”

Section: Exercise-related Oxidative Stressmentioning

confidence: 99%

Oxidative stress in equine medicine - Current knowledge

Kirschvink

Lekeux

2002

PHK

View full text Add to dashboard Cite

SummaryOxidative stress has become of increasing interest in research and human medicine. Oxidants or reactive oxygen species are generated by metabolic reactions and play an important role for the immune defence and the "redox-regulation" of gene expression. In physiological conditions, different categories of antioxidants maintain the equilibrium between the pro-oxidant burden and the endogenous antioxidant defence. However, when an imbalance between oxidants and antioxidants in favour of oxidants occurs, oxidative stress might induce irreversible lesions that can be evidenced and characterised by oxidant markers. In equine medicine, it has been shown that oxidative stress is occurring in lower airway disease and equine motor neuron disease. Moreover, adapted antioxidants therapy appears to have a beneficial effect on the antioxidant-oxidant imbalance. Exercise-induced oxidative stress and its impact on animal welfare and performance are also under investigation in equine sports medicine. Oxidative stress has been demonstrated to depend on exercise type, intensity and duration. It might be modulated by training and/or adapted antioxidant therapy, aiming to maintain the antioxidant-oxidant equilibrium and thereby to improve animal welfare.

show abstract

Role of exercise and ascorbate on plasma antioxidant capacity in thoroughbred race horses

Cited by 100 publications

References 25 publications

Plasma antioxidant status and cell injury after severe physical exercise

Plasma antioxidant status and cell injury after severe physical exercise

Effect of Dietary Supplementation with Vitamin E and Selenium in Thoroughbred Horses on the Concentration of F2-isoprostanes in the Blood Plasma as a Marker of Lipid Peroxidation

Oxidative stress in equine medicine - Current knowledge

Contact Info

Product

Resources

About