Role of enzymatic free radical scavengers in management of oxidative stress in autoimmune disorders

Srivastava, Shikha; Singh, Deependra; Patel, Satish; Singh, Manju Rawat

doi:10.1016/j.ijbiomac.2017.03.100

Cited by 71 publications

(45 citation statements)

References 119 publications

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“…It is also well known that hyperglycemia is the major factor responsible for the activation of oxidative stress in diabetes and reactive oxygen species (ROS) formation is a direct consequence of hyperglycemia . ROS promotes cellular oxidation and, in turn, accumulation of free radicals cause an upregulation of inflammatory cell phenotype, elevation of proinflammatory cytokines, chemokines, tumor necrosis factor and transcription pathways such as nuclear factor‐kappa B (NF‐.B) and hypoxia‐related factors such as hypoxia inducible factor (HIF)‐1 α . HIF‐1α an essential factor in the adaptive response to hypoxic conditions and its activity is transcriptionally mediated by NF‐.B .…”

Section: Introductionmentioning

confidence: 99%

Morphometric and histopathological evaluation of the effect of grape seed proanthocyanidin on alveolar bone loss in experimental diabetes and periodontitis

Toker

Yüce

Alpan

et al. 2018

J of Periodontal Research

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Section: Introductionmentioning

confidence: 99%

Morphometric and histopathological evaluation of the effect of grape seed proanthocyanidin on alveolar bone loss in experimental diabetes and periodontitis

Toker

Yüce

Alpan

et al. 2018

J of Periodontal Research

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“…Excessive generation of reactive oxygen species (ROS) in cells or tissues results from noxious stimulation by atmospheric pollution, smoke, drugs and trauma can lead to disequilibrium of oxidant and antioxidant systems, resulting in tissue damage, which in turn causes additional oxidative stress (6). Based on previous studies, oxidative stress is likely to play an important role in the generation and development processes of nasal polyps (7)(8)(9).…”

Section: Introductionmentioning

confidence: 99%

PTEN promotes apoptosis of H2O2‑injured rat nasal epithelial cells through PI3K/Akt and other pathways

2017

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Chronic rhinosinusitis (CRS) is a form of chronic inflammation of the nasal cavity and paranasal sinus with multi‑causal pathogenesis, including oxidative stress. Several lines of evidence have demonstrated that the phosphatase and tensin homolog gene (PTEN) can inhibit the activation of phosphoinositide 3‑kinase (PI3K) to affect phosphorylation of Akt. Importantly, the PI3K/PTEN/Akt signaling pathway is associated with various types of tumors, chronic inflammatory diseases, and autoimmune disease through its regulation of cell growth, apoptosis, proliferation, and metabolism. This in vitro study aimed to investigate the role of PTEN and the relationship between PTEN and the PI3K/Akt pathway in nasal epithelial cells under oxidative stress. H2O2 treatment was applied to induce a cell injury model of oxidative stress in rat nasal epithelial cells. Cells were divided into control, H2O2, H2O2+PTEN, and H2O2+siPTEN groups. Cell viability was measured using the CCK‑8 assay, and reactive oxygen species (ROS) levels and apoptosis rates were analyzed by flow cytometry (FCM). Oxidative parameters, including ROS, catalase (CAT), and malondialdehyde (MDA), were tested by enzyme‑linked immunosorbent assay (ELISA). The expression of apoptosis‑related genes and PI3K/Akt pathway was assayed by quantitative PCR (qPCR) and western blot. In H2O2‑injured cells, oxidative stress, due to increased ROS levels and apoptosis rates, was induced, and PTEN aggravated the injury. The levels of both p‑Akt and PTEN in H2O2‑injured cells were positively correlated and higher than in control cells. Unknown regulatory protein(s) may exist in the PI3K/PTEN/Akt pathway or the PTEN and PI3K/Akt pathways may be two independent signaling pathways that have cross interactions.

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“…ROS are produced in response to inflammation, aging, radiation, air pollution and exposures to different physicochemical conditions (TPA et al, 2004). RNS are generated from nitric oxide (NO) and peroxides (OONO), produced by the mitochondrial respiratory chain when the cell is oxygen deficie nt (hypoxia) (Srivastava, Singh, Patel, & Singh, 2017). When ROS/RNS reacts with biomolecules, they can result in the damage of proteins, oxidation of membrane phospholipids, mutations in DNA and modifications in Low Density Lipoproteins (LDL) (Baran, Zeigler, & Tridandapani, 2004).…”

Section: Oxidative Stressmentioning

confidence: 99%

“…The generation of superoxide anion in the cell has been associated with different diseases such as chronic inflammation and various cancers (K. Shah, R. G. Kumar, S. Verma, 2001;Rahman et al, 2012;Srivastava et al, 2017). When superoxide (free radical) react with lipids, the lipids undergo peroxidation, hence forming lipid peroxides which decompose to form several toxic products such as malondialdehyde (MDA) (Beckman & Koppenol, 1996).…”

Section: Superoxide (O 2 ─• )mentioning

confidence: 99%

“…Hydrogen peroxide (H 2 O 2 ) is a relatively stable ROS with a long half-life of 60 seconds compared to O 2 ─• . It is derived from dismutation of superoxide radical (O 2 ─• ) from the electron transport chain (Halliwell & Gutteridge, 2015) and enzymatically activated by mitochondria superoxide dismutase (SOD) enzyme (Srivastava et al, 2017). H 2 O 2 is one of the non-radical ROS because it has no unpaired electrons, hence far less reactive compared to superoxide radical.…”

Section: Hydrogen Peroxide (H 2 O 2 )mentioning

confidence: 99%

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Effects of Hydrolyzed Oat Bran Proteins on Free Radicals, Toxic Metals and Lipid Oxidation

Shittu¹

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Transition metals play an important role in wide variety of biological processes in living system. Exposure to toxic metals, specifically arsenic (As) and chromium (Cr), has been associated with inflammation, cellular damage, and cardiovascular diseases as a result of As/Cr-mediated oxidative stress. In recent years, food proteins have been of major interest because in addition to being sources of essential amino acids they can also provide bioactive peptides. Several studies have found that these food peptides exhibit antioxidant, anti-inflammatory and anti-hypertensive properties. Oat is the sixth consumed type of cereal and its use in human nutrition has been increasing in recent years because of the discovery of functional and biological activity of some of its constituents. In the present study, medium oat bran was pre-treated with cellulase and viscozyme to degrade the carbohydrase, followed by the hydrolysis of the isolated proteins with proteases; alcalase (AL), papain (PA), flavourzyme (FL), and protamex (PRO). We investigated the characteristics of the oat bran protein isolates (OBPI) and the oat bran protein hydrolysates (OBPH), their ability to reduce the toxicity of arsenic and chromium as well as their radical scavenging properties. OBPI samples include cellulase (CELL) and viscozyme (VISC) pre-treated protein isolates respectively. OBPH samples were named based on the carbohyrase and protease used in hydrolysis; CAL, CPA, CFL, CPRO, VAL, VPA, VFL, VPRO. Results showed that OBPIs possessed higher protein contents (80%) and higher molecular weight (MW > 20kDa) than the OBPH with an average of 45% protein content and MW < 15kDa. OBPIs exhibited higher levels of aromatic amino acid residue (p < 0.05) compared to OBPHs. Meanwhile, aromatic amino acid contents varied among OBPHs due to difference in specificities of proteases. In the peroxyl radical scavenging (ORAC) assay, OBPHs from viscozyme treated brans performed better than those from cellulase. VFL and VAL had the highest ORAC value ranging from 565 ± 80 -640 ± 57 µM TE/g respectively compared to 406.3 ± 43 -506.7 ± 65 µM TE/g for hydrolysates from cellulase treated bran. VISC and iv CELL isolates had lower ORAC values ranging from 107.9 ± 13.5 -305.8 ± 28.3 µM TE/g respectively compared to OBPHs. Hydrolysis increased peroxyl radical scavenging activities (ORAC) but lowered hydroxyl radical (HO•) potentials while only minor changes were observed in the superoxide anion radical scavenging power. OBPIs and OBPHs had no effect on lipid peroxides in non-stored samples (NS) and -20 0 C storage, however storage at 4 0 C, cellulase treated OBPHs had lower concentration of lipid peroxide compared to viscozyme.Data on the effect of OBPI and OBPH on arsenic showed that at -20 0 C, treated samples had higher concentration of As (III) compared to NS, but mostly lower concentration compared to 4 0 C.Concentration of As (V) in treated samples is mostly lower compared to NS and 4 0 C. Non-stored samples (NS) treated with CELL and CFL had the highest (p < 0.05) c...

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Role of enzymatic free radical scavengers in management of oxidative stress in autoimmune disorders

Cited by 71 publications

References 119 publications

Morphometric and histopathological evaluation of the effect of grape seed proanthocyanidin on alveolar bone loss in experimental diabetes and periodontitis

Morphometric and histopathological evaluation of the effect of grape seed proanthocyanidin on alveolar bone loss in experimental diabetes and periodontitis

PTEN promotes apoptosis of H2O2‑injured rat nasal epithelial cells through PI3K/Akt and other pathways

Effects of Hydrolyzed Oat Bran Proteins on Free Radicals, Toxic Metals and Lipid Oxidation

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