Role of Endoplasmic reticulum apoptotic pathway in testicular Sertoli cells injury induced by Carbon disulfide

Guo, Yinsheng; Ji, Jiajia; Wang, Wei; Dong, Yu; Zhang, Zhen; Zhou, Yijun; Chen, Guoyuan; Cheng, Jinquan

doi:10.1016/j.chemosphere.2015.02.058

Cited by 8 publications

(2 citation statements)

References 44 publications

(43 reference statements)

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“…Cell apoptosis plays an important role in the pathophysiology of respiratory diseases and pulmonary inflammation (El Kebir et al 2012;Pierce et al 2007). Flow cytometry is the most common method used to verify cell apoptosis (Guo et al 2015). Exploring whether DEHP leads to apoptosis in 16HBE cells could contribute to further understanding of DEHP-induced respiratory diseases.…”

Section: Discussionmentioning

confidence: 99%

Analysis of toxicity effects of Di-(2-ethylhexyl) phthalate exposure on human bronchial epithelial 16HBE cells

Guo

et al. 2017

Cytotechnology

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Recent studies have indicated that Di-(2-ethylhexyl) phthalate (DEHP), the most commonly used plasticizer in daily-life products, could be dispersed in indoor air and induce human exposure via inhalation. DEHP has been reported to have effects on the respiratory system in both animal and human researches. The toxicity effects of DEHP exposure on cell proliferation, cell cycle progression, apoptosis, global DNA methylation and the expression levels of DNA methyltransferases (DNMTs) were investigated in this study, using human epithelial cell line 16HBE as an in vitro model. Cells were treated with DEHP at doses of 0, 0.125, 0.5 and 2 mmol/L for 48 h. Cell proliferation, cell cycle and apoptosis were tested by MTT assay and flow cytometer, respectively. The obtained results showed decreased living cell number and cell viability following DEHP exposure at the dose of 2 mmol/L. DEHP also inhibited the cell cycle progression of G1 phase and induced a significant increase in cell apoptosis in 16HBE cells. DEHP exposure could induce cell proliferation inhibition in 16HBE cells via the blocking of cell cycle progression and accelerated cell apoptosis. In addition, decreased global DNA methylation levels and expression levels of DNMTs were observed in DEHP-treated groups which revealed possible epigenetic effects of DEHP.

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Section: Discussionmentioning

confidence: 99%

Analysis of toxicity effects of Di-(2-ethylhexyl) phthalate exposure on human bronchial epithelial 16HBE cells

Guo

et al. 2017

Cytotechnology

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“…ER stress (ERS) is closely related to obesity-induced metabolic dysfunction, including testicular reproductive dysfunction (Martin-Jimeńez et al, 2017;Park et al, 2017). Studies have shown that ERS injury is one of the main causes of abnormal spermatogenesis and persistent excessive ERS can interfere with the testicular germ cell-mediated secretion of related proteins or cytokines, thereby affecting testicular spermatogenesis (Guo et al, 2015). Studies have shown that whenever reactive oxygen species (ROS) release exceeds the capacity of endogenous antioxidants, oxidative stress occurs, which is inextricably linked to obesity and related diseases (especially insulin resistance and type 2 diabetes) (Niemann et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Effects of Lycium barbarum Polysaccharide on Endoplasmic Reticulum Stress and Oxidative Stress in Obese Mice

et al. 2020

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Background: The incidence of obesity-associated decline in male fertility has increased over the years. Lycium barbarum polysaccharide (LBP), a natural plant polysaccharide extracted from the Chinese herb L. barbarum has shown promising therapeutic effects in overcoming the same.Aim: This study aimed to investigate the protective effect of LBP on the testes of obese mice.Methods: Following administration of LBP to high-fat diet-induced obese mice for 35 days, serum, sperm, and testis samples were obtained for subsequent experiments. Biochemical analysis and sex hormone content determination were performed to observe changes in glycolipid metabolism and testosterone levels, respectively, in the blood. Hematoxylin and eosin staining were carried out to assess the pathological changes in the testicular tissue. Oxidative stress levels were detected using enzyme-linked immunosorbent assay and expression levels of endoplasmic reticulum stress markers were determined using western blot in the testicular tissue.Results: Our results suggested that LBP reduced glucose levels and insulin resistance, increased testosterone levels and insulin sensitivity, and decreased testicular oxidative stress and pathological damage in obese mice. In addition, LBP down-regulated the expression of p-eIF2a, GRP78, and CHOP in the testicular tissues of obese mice. Conclusion:Our results show that LBP is a potential novel drug for preventing male infertility caused by obesity.

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Environmental Factors

Leisegang¹,

Henkel²

2020

Male Infertility

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Role of Endoplasmic reticulum apoptotic pathway in testicular Sertoli cells injury induced by Carbon disulfide

Cited by 8 publications

References 44 publications

Analysis of toxicity effects of Di-(2-ethylhexyl) phthalate exposure on human bronchial epithelial 16HBE cells

Analysis of toxicity effects of Di-(2-ethylhexyl) phthalate exposure on human bronchial epithelial 16HBE cells

Effects of Lycium barbarum Polysaccharide on Endoplasmic Reticulum Stress and Oxidative Stress in Obese Mice

Environmental Factors

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