Role of Dietary Antioxidants to Protect against DNA Damage in Adult Dogs

Abstract

We studied the effects of feeding an antioxidant blend of vitamins, minerals and carotenoids to a mixed adult dog population (n = 40, mean 4.4 +/- 1.85 y) for a 16-wk period. Compared to the control group of dogs (n = 20), the antioxidant (AOX)-supplemented group of dogs (n = 20) demonstrated significant increases in plasma levels of vitamin E and taurine by 4 wk of supplementation (P < 0.01) and total antioxidant activity (as measured by ferric-reducing antioxidant power assay) by 8 wk of supplementation (P < 0.05). Following 8 wk of supplementation, the AOX-supplemented dogs also showed significant reductions in both endogenous and exogenous DNA damage (P < 0.005) compared to that of the control dogs, as measured by the comet assay. Over an 8-wk rabies vaccination course that started at 8 wk supplementation, the AOX-supplemented dogs also demonstrated significantly higher vaccine-specific virus-neutralizing antibody levels at 2, 4 and 6 wk postvaccination (P < 0.05) and a tendency toward establishing a vaccine-specific antibody response quicker than did the control group of dogs. These findings in dogs suggest that antioxidant supplementation can achieve sustained increases in circulating levels of antioxidants that exert a protective effect by a decrease in DNA damage, leading to improved immunological performance. These findings also have implications in a wider context where free-radical damage has been associated with a variety of degenerative disorders and the aging process in general.

Effects of antioxidants on the aging inner ear

“…In an effort to mitigate this possibility, dietary mitochondrial metabolites, antioxidants and a mixture of fruits and vegetables were fed to beagle dogs and it was demonstrated that this regime can reduce oxidative protein and DNA damage (Heaton et al, 2002) and prevent cognitive dysfunction (Conte et al, 2004;Cotman et al, 2002;Farr et al, 2003;Milgram et al, 2002) in aged dogs. We, therefore, hypothesized that this combination of dietary antioxidants and mitochondrial metabolites may reduce the magnitude of cellular losses seen in aged cochleae that result in age-related hearing loss and presbycusis.…”

Effect of mineral supplementation on humoral immunity against rabies vaccine in dog pups

“…This shows that mineral supplementation in the animal being vaccinated may be an important additive especially in post bite cases of rabies wherein an early antibody response is of utmost importance for the preventing the mortalities. Similarly, significantly greater (P<0.05) production of rabies specific antibodies at 2, 4 and 6 weeks post vaccination in group of dogs supplemented with antioxidant nutrients as compared to the control group was recorded (Heaton et al 2002). Proper functioning of immune system requires appropriate levels of macro as well as micro minerals to be included in the diet of animals.…”

Evaluation of antigenotoxic potential of salvianolic acid B with hydrogen peroxide on human peripheral blood leukocytes in vitro

“…U kotretmanu, uzorci su istovremeno tretirani vodonik-peroksidom u koncentraciji od 100 μM i Sal B u tri različite koncentracije (12,5 može sprovoditi na gotovo svakom tipu ćelija kao i na leukocitima periferne krvi koji su predmet tretmana u ovom radu. Komet testom se mogu uspešno proceniti interakcije antioksidanasa i genotoksičnih agenasa (23), a pouzdano se koristi i za ispitivanje efekta određenih supstanci na očuvanje integriteta genetičkog materijala (24,25,26). Komet test su prvobitno razvili Ostling i Johanson (27) …”