Role of Dehalogenases in Aerobic Bacterial Degradation of Chlorinated Aromatic Compounds

Arora, Pankaj; Bae, Hyeun‐Jong

doi:10.1155/2014/157974

Cited by 15 publications

(14 citation statements)

References 71 publications

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“…A diverse array of enzymes catalyze oxidative haloaromatic dehalogenations, including flavin-dependent oxygenases, cytochrome P450 oxidases, and dehaloperoxidases. 400–402 The best studied oxidative dehalogenase is the pentachlorophenol-4-monooxgygenase, PcpB, an O 2 /NADPH-dependent flavoprotein monooxygenase that catalyzes the conversion of 109 to tetrachloroquinone in the bacterial catabolism of 109 (Figure 37A). 403 In contrast to the other dehalogenating enzymes described in this review, many enzymes that catalyze oxidative dehalogenases are promiscuous against a broad range of substrates.…”

Section: Enzymatic Dehalogenationmentioning

confidence: 99%

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Enzymatic Halogenation and Dehalogenation Reactions: Pervasive and Mechanistically Diverse

et al. 2017

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Naturally produced halogenated compounds are ubiquitous across all domains of life where they perform a multitude of biological functions and adopt a diversity of chemical structures. Accordingly, a diverse collection of enzyme catalysts to install and remove halogens from organic scaffolds has evolved in nature. Accounting for the different chemical properties of the four halogen atoms (fluorine, chlorine, bromine, and iodine) and the diversity and chemical reactivity of their organic substrates, enzymes performing biosynthetic and degradative halogenation chemistry utilize numerous mechanistic strategies involving oxidation, reduction, and substitution. Biosynthetic halogenation reactions range from simple aromatic substitutions to stereoselective C-H functionalizations on remote carbon centers and can initiate the formation of simple to complex ring structures. Dehalogenating enzymes, on the other hand, are best known for removing halogen atoms from man-made organohalogens, yet also function naturally, albeit rarely, in metabolic pathways. This review details the scope and mechanism of nature’s halogenation and dehalogenation enzymatic strategies, highlights gaps in our understanding, and posits where new advances in the field might arise in the near future.

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Section: Enzymatic Dehalogenationmentioning

confidence: 99%

“…406 To maintain the focus of the review to address dehalogenation mechanisms in the context of substrate-specific enzymes, the reader is referred to a series of recent reviews on the topic of oxidative enzymatic dehalogenation. 400–402 …”

Section: Enzymatic Dehalogenationmentioning

confidence: 99%

Enzymatic Halogenation and Dehalogenation Reactions: Pervasive and Mechanistically Diverse

et al. 2017

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“…RdhA and Rdase, have been shown to be involved in the aerobic dehalogenation of aromatic organohalide compounds(Arora and Bae 2014). Three bacterial species identified in the present study, Pseudomonas spp.…”

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confidence: 58%

A coupled UV photolysis-biodegradation process for the treatment of decabrominated diphenyl ethers in an aerobic novel bioslurry reactor

Chang

Chen

Chou

et al. 2020

Environ Sci Pollut Res

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The commercial flame retardant is an emerging contaminant (EC) commonly found in soils and sediments. A coupled UVphotolysis-biodegradation process was used to decompose decabromodiphenyl ether (BDE-209) in clay slurries. A novel bioslurry bioreactor (NBB) was employed in which BDE-209 degradation was maximized by the simultaneous application of LED UVA irradiation and biodegradation by a mixed bacterial culture. The rate of BDE-209 degradation decreased in the order: coupled UV photolysis-biodegradation (1.31 × 10-2 day-1) > UV photolysis alone (1.10 × 10-2 day-1) > biodegradation alone (1.00 × 10-2 day-1). Degradation intermediates detected included hydroxylated polybrominated diphenylethers, partially debrominated PBDE congeners and polybrominated dibenzofuran. The UV-resistant bacterial strains isolated that could utilize BDE-209 as a sole carbon source included Stenotrophomonas sp., Pseudomonas sp., and Microbacterium sp. These strains encoded important functional genes such as dioxygenase and reductive dehalogenases. Continuous UV irradiation during the NBB process affected various biochemical oxidative reactions during PBDEs biodegradation. Simultaneous photolysis and biodegradation in the NBB system described reduces operational time, energy, expense, and maintenance-demands required for the remediation of BDE-209 when compared to sequential UV-biodegradation process or to biodegradation alone. Keywords Coupled UV photolysis-biodegradation. Decabromodiphenyl ether. Novel bioslurry bioreactor. UV-resistant bacterial strains. Polybrominated dibenzofuran Highlights • A time, energy, and cost-saving technique using a coupled UV photolysis-biodegradation process for degrading BDE-209 in a novel bioslurry bioreactor. • Identification of isolated UV-resistant bacterial strains that potentially are able to utilize PBDEs as sole carbon source and the analysis of related functional genes. • The prediction of a possible pathway for BDE-209 degradation by coupled photolysis-biodegradation.

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“…Results from the optimization study of biodegradation indicated that the maximum rate of PAH removal occurred at 100 mg L −1 of PAHs, 10% bacteria concentration, pH 7.0, and 30 °C (Othman et al 2016 ). For the degradation of pesticides, bacteria must possess enzymes that cleave the ring of the aromatic halogen compound (PCP) (Arora et al 2010 ; Arora and Bae 2014 ; Zhou et al 2008 ).…”

Section: Resultsmentioning

confidence: 99%

Microbial communities in pesticide-contaminated soils in Kyrgyzstan and bioremediation possibilities

Döölotkeldieva

Konurbaeva

Bobusheva

2017

Environ Sci Pollut Res

103

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In Kyrgyzstan, many former storehouses and dump sites for obsolete pesticides exist. In 2009/2010, an inventory and assessment of these sites including risks of environmental hazard has been conducted by FAO and the World Bank. Monitoring revealed high concentration of pesticides listed as persistent organic pollutants (POPs). The purpose of this research was to study the microbial structural complexes of the pesticide-contaminated soils in these dumping zones, and to search for and select microorganism’s destructors with cytochrome P450 genes for pesticide degradation. Culture-dependent and culture-independent approaches were used to determine the taxonomic composition of these bacterial communities. The universal primer set for the 16S ribosomal RNA (rRNA) gene and the specific primer set P450R were used to amplify the cytochrome P450 hydroxylase gene. In soils from Suzak A and B and soils from Balykchy dumping sites, the bacteria from the Actinobacteria phylum (Micrococcus genus) were dominant. These bacteria made up 32–47% of the indigenous local microflora; bacteria species from the Pseudomonas genus (Gammaproteobacteria phylum) made up 23% in Suzak, 12% in Balykchy soils. Bacillus species from the Firmicutes phylum were found only in Suzak soils. The 16S rRNA analyses and the specific primer set P450R have revealed bacteria with cytochrome genes which are directly involved in the degradation process of organic carbon compounds. Experiments were carried out to help select active degraders from the bacterial populations isolated and used to degrade Aldrin in laboratory. Active bacterial strains from the Pseudomonas fluorescens and Bacillus polymyxa population were selected which demonstrated high rates of degradation activity on Aldrin.

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Role of Dehalogenases in Aerobic Bacterial Degradation of Chlorinated Aromatic Compounds

Cited by 15 publications

References 71 publications

Enzymatic Halogenation and Dehalogenation Reactions: Pervasive and Mechanistically Diverse

Enzymatic Halogenation and Dehalogenation Reactions: Pervasive and Mechanistically Diverse

A coupled UV photolysis-biodegradation process for the treatment of decabrominated diphenyl ethers in an aerobic novel bioslurry reactor

Microbial communities in pesticide-contaminated soils in Kyrgyzstan and bioremediation possibilities

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