Role of Dectin‐1 receptor on cytokine production by human monocytes challenged with <i>Paracoccidioides brasiliensis</i>

Romagnolo, Alexandre Giannecchini; Silva, Juliana Carvalho de Quaglia e; Coletta, Amanda Manoel Della; Gardizani, Taiane Priscila; Martins, Ana Teresa Loyola; Romagnoli, Graziela Gorete; Kaneno, Ramon; Soares, Ângela Maria Victoriano de Campos; Faveri, Júlio De; Dias-Melício, Luciane Alarcão

doi:10.1111/myc.12725

Cited by 12 publications

(12 citation statements)

References 77 publications

(175 reference statements)

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“…In PCM, many studies have contributed to the understanding of Dectin-1 receptor functions in human phagocytes. Studies that evaluated the participation of the receptor used different protocols, demonstrating results such as receptor participation in fungal recognition, induction of effector mechanisms, and production of cytokines such as TNF- α [4, 6, 7, 9–11, 35–37]. In experimental models of the disease, there are conflicting data showing the participation of the Dectin-1 receptor in susceptibility and in PCM resistance [5, 8, 11, 38–40].…”

Section: Discussionmentioning

confidence: 99%

“…At the beginning of the infection, neutrophils and monocytes are recruited to the injured site. The fungus is initially recognized by Pattern Recognition Receptors (PRRs), and among them, the Dectin-1 receptor, a member of the C-type lectin-like receptors (CLR) can bind specifically to β -1,3 glucans in the fungal cell wall [4–9]. The latest studies have characterized the different activation pathways of the Dectin-1 receptor in response to P .…”

Section: Introductionmentioning

confidence: 99%

“…The latest studies have characterized the different activation pathways of the Dectin-1 receptor in response to P . brasiliensis that triggers cellular activation leading to a modulatory function through the production of chemokines and cytokines such as TNF- α , IL-1 β , IL-18, IL-12, IL-8, IL-17, and IL-10 [9–11].…”

Section: Introductionmentioning

confidence: 99%

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Involvement of the Dectin-1 Receptor upon the Effector Mechanisms of Human Phagocytic Cells against Paracoccidioides brasiliensis

Silva

Coletta

Gardizani

et al. 2019

Journal of Immunology Research

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Paracoccidioidomycosis (PCM), a systemic mycosis endemic in Latin America, occurs after inhalation of mycelial components of Paracoccidioides spp. When the fungus reaches the lungs and interacts with the alveolar macrophages and other cells, phagocytic cells such as neutrophils and monocytes are immediately recruited to the injured site. The interaction between surface molecules of pathogens and homologous receptors, present on the surface membrane of phagocytes, modulates the innate immune cell activation. Studies have shown the importance of fungal recognition by the Dectin-1 receptor, which can induce a series of cellular protective responses against fungi. The objective of the present study was to evaluate Dectin-1 receptor expression and the effector mechanisms of human monocytes and neutrophils activated or not with different cytokines, such as IFN-γ, TNF-α, and GM-CSF, followed by the challenge with Paracoccidioides brasiliensis (P. brasiliensis or Pb265). Therefore, analysis of Dectin-1 receptor expression was done by flow cytometry whereas the effector mechanisms were evaluated by fungal recovery by colony-forming unit (CFU) counting and hydrogen peroxide (H2O2) production. Our results showed that, after treatment with IFN-γ, TNF-α, and GM-CSF and challenge with Pb265, cells, especially monocytes, demonstrated an increase in Dectin-1 expression. Both types of cells treated with the cytokines exhibited a decreased fungal recovery and, conversely, an increased production of H2O2. However, when cultures were treated with an anti-Dectin-1 monoclonal antibody, to block the P. brasiliensis binding, a decrease in H2O2 production and an increase in fungal recovery were detected. This effect was observed in all cultures treated with the specific monoclonal antibody. These results show the involvement of the Dectin-1 receptor in fungal recognition and its consequent participation in the induction of the killing mechanisms against P. brasiliensis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Involvement of the Dectin-1 Receptor upon the Effector Mechanisms of Human Phagocytic Cells against Paracoccidioides brasiliensis

Silva

Coletta

Gardizani

et al. 2019

Journal of Immunology Research

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“…It recognizes β-glucan, present in the fungal cell wall, and therefore participates in the recognition of many fungal species, including P. brasiliensis ( Balderramas et al, 2014 ; Feriotti et al, 2015 ; Loures et al, 2015 ; Bachiega et al, 2016 ). Recently, Romagnolo et al (2018) demonstrated that this receptor participates in cytokine secretion by monocytes when stimulated in vitro with P. brasiliensis 265 isolate. Our results show that this receptor is not involved in ArtinM activation of neutrophils infected with Pb18 isolate.…”

Section: Discussionmentioning

confidence: 99%

Effect of ArtinM on Human Blood Cells During Infection With Paracoccidioides brasiliensis

et al. 2018

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Infections caused by fungi are prominent in our environment and can be potentially fatal. paracoccidioidomycosis (PCM), caused by fungi of the Paracoccidioides genus, is the most frequent systemic mycosis in Brazil and the main cause of death among immunocompetent individuals. The antifungal therapy for PCM is usually effective but side effects and relapses are often reported. The latter could be avoided with alternative or complementary therapies aimed at boosting the immune response to combat this pathogen. Recent reports have pointed at the importance of an effective cellular immune response, with the participation of Th1 cells, in the resistance to and control of Paracoccidioides infection. The ArtinM lectin, extracted from jackfruit (Artocarpus heterophyllus) seeds, exhibits immunomodulatory activity against several intracellular pathogens, including Paracoccidioides brasiliensis, by promoting the development of a Th1 immune response. The aim of this work was to characterize the effect of ArtinM on peripheral blood cells of patients with PCM and on those of control individuals infected with fungal yeasts cells in vitro. Our results demonstrate that ArtinM activates human neutrophils in vitro, leading to an increase in cytokine production and CD54 expression. ArtinM activated P. brasiliensis-infected neutrophils from both healthy individuals and patients with PCM. This activation was not dependent on the dectin-1 receptor, because pre-incubation with laminarin, a dectin-1 receptor blocker, did not reverse the activated state of the cells. ArtinM also stimulated human peripheral blood mononuclear cells to secrete pro-inflammatory Th1-related cytokines, which are protective against Paracoccidioides infection. These data support the immunostimulatory action of ArtinM and encourage new studies using the lectin for the immunotherapy of PCM.

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“…The PRRs could be intracellular cytoplasmic or extracellular transmembrane receptors [11,15], and their bond with the PAMPs triggers many processes including the actuation of the adaptive immune response. For instance, the detection of PAMPs by Dectin-1 CLR modulates the uptake of the microorganisms via phagocytosis, leading to the production of inflammatory cytokines such as IL-12 [15,16], a fundamental cytokine involved in the activation of T Helper 1 (Th1) cells [1]. Indeed, the activation of PRRs initiates signaling cascades that promote the transcription of targeted gene encoding for cytokines, interferons, pro-inflammatory or microbicide proteins [17,18].…”

Section: Innate Immunitymentioning

confidence: 99%

Trained Immunity at a Glance; A Review on the Innate Immune Memory and its Potential Role in Infections, Diseases and New Therapeutic Strategies

Incalcaterra

Domínguez

2020

Adv. J. Grad. Res.

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Despite the existence of two different branches of immunity, innate and adaptive, it has been described that both systems are characterized by the establishment of memory responses. Indeed, it has been shown that cells belonging to the innate immune system can express a so-called “trained” memory, although it has different features from the adaptive immune memory. Adaptive memory is a long-lasting specific memory whereas innate memory involves non-specific responses which enhance the immune response during a second reinfection. However, many aspects of the trained immunity are still unclear. Metabolic and epigenetic reprogramming have been pointed as the two processes responsible for the establishment of the innate memory. Trained immunity seems to be responsible for the heterologous effect of many vaccines such as BCG, thus giving insights for the development of new therapies. Although its potential beneficial role, trained immunity could also have detrimental effects that might worsen the progress of certain diseases. The purpose of this literature review is to provide an in-depth review on the major characteristics of trained immunity, describing the main pathways at the basis of the evolution and establishment of memory in innate cells. In addition, the present review assesses the modern evidence of the impact of trained immunity in health and disease, strengthening the hypotheses that this innate memory may be considered both in the formulation of new therapeutic strategies and in the current therapeutic approaches.

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Role of Dectin‐1 receptor on cytokine production by human monocytes challenged with Paracoccidioides brasiliensis

Cited by 12 publications

References 77 publications

Involvement of the Dectin-1 Receptor upon the Effector Mechanisms of Human Phagocytic Cells against Paracoccidioides brasiliensis

Involvement of the Dectin-1 Receptor upon the Effector Mechanisms of Human Phagocytic Cells against Paracoccidioides brasiliensis

Effect of ArtinM on Human Blood Cells During Infection With Paracoccidioides brasiliensis

Trained Immunity at a Glance; A Review on the Innate Immune Memory and its Potential Role in Infections, Diseases and New Therapeutic Strategies

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