Role of Cytokinins in Development and Metabolism of Barley Leaves. VII. Effect of Light and Kinetin on the Transcriptional Activity of Nuclei from Etiolated Leaves

Schneider, J.; Gruszka, Malgorzata; Legocka, Jolanta; Szweykowska, A.

doi:10.1016/s0015-3796(83)80054-7

Cited by 3 publications

(2 citation statements)

References 27 publications

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“…This difference may be due to the fact that in the present experiments, green, whitelight-grown plants were used, whereas etiolated plants were used in the previous study; also the nuclear isolation protocols differed. There have been reports of stimulation of ribosomal RNA synthesis by BA in isolated barley nuclei [16]. In this work it was noted that the stage of leaf development was critical for the effect to be observed and the stimulation by BA was not large, i.e., less than a 50% increase in ct-amanitin-insensitive RNA synthesis.…”

Section: Discussionmentioning

confidence: 77%

Cytokinin modulation of LHCP mRNA levels: the involvement of post-transcriptional regulation

1988

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When white-light-grown Lemna gibba plants are placed in the dark, the levels of mRNAs for two nuclear-encoded chloroplast proteins, the small subunit of ribulose-1,5-bisphosphate carboxylase (SSU) and the major chlorophyll a/b-binding protein of light-harvesting complex II (LHCP), decline to a small fraction of their previous level. We have reported [4] that red light (R), acting through phytochrome, and benzyladenine (BA), a synthetic cytokinin, independently stimulate accumulation of both mRNAs in the dark. Here, we have analyzed the products of transcription in isolated nuclei to determine if cytokinins act primarily through stimulation of transcription or if post-transcriptional processes are involved. We find that BA pretreatment may slightly stimulate transcription of LHCP RNA either with or without a red-light treatment. However, the effects of BA on the LHCP RNA accumulation were much greater than on transcription. Two naturally occurring cytokinins are also effective in increasing the mRNA abundance. We therefore conclude that, in Lemna, post-transcriptional processes are important in regulation of the LHCP RNA by cytokinins.

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Section: Discussionmentioning

confidence: 77%

Cytokinin modulation of LHCP mRNA levels: the involvement of post-transcriptional regulation

1988

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“…There is ample evidence that both cytokinin and light promote plastid development [18,19]. Cytokinin and light are also reported to stimulate or inhibit [ 14C]UTP incorporation into acid-insoluble products in isolated barley leaf nuclei depending upon the age of the leaves [21 ]. Since the cytokinin effect is dependent upon the presence of light, it is possible that cytokinin induces conformational changes in trans-acting factors which in turn interact with light-responsive factors to enhance gene expression.…”

Section: Discussionmentioning

confidence: 99%

Cytokinin enhancement of the light induction of nitrate reductase transcript levels in etiolated barley leaves

Ertl

Chen

1990

Plant Mol Biol

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To investigate the molecular mechanism of cytokinin regulation of nitrate reductase (NR) activity, the influence of benzyladenine (BA) on the level of NR transcript was studied in etiolated barley leaves using a barley NR cDNA as a probe. Northern blot analyses of the levels of NR poly (A)+ RNA indicate that the amount present is proportional to the concentration of BA (2 x 10(-8) to 2 x 10(-4) M) applied to the leaves. Enhancement of NR mRNA by 2 x 10(-5) M BA was clearly detected after 15 minutes of exposure of the leaves to light. The enhancement is cytokinin-specific and adenine is ineffective. Brief treatment with the protein synthesis inhibitor, cycloheximide, inhibited BA-enhanced NR activity but did not inhibit BA-enhanced NR transcript level, thus the enhancement was independent of concurrent protein synthesis. Nuclear runoff transcription studies showed that the enhancement of NR mRNA was at least partially due to increased transcription rates.

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Cytokinin-controlled ribulose 1,5-bisphosphate carboxylase gene expression in pumpkin cotyledons

et al. 1985

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Role of Cytokinins in Development and Metabolism of Barley Leaves. VII. Effect of Light and Kinetin on the Transcriptional Activity of Nuclei from Etiolated Leaves

Cited by 3 publications

References 27 publications

Cytokinin modulation of LHCP mRNA levels: the involvement of post-transcriptional regulation

Cytokinin modulation of LHCP mRNA levels: the involvement of post-transcriptional regulation

Cytokinin enhancement of the light induction of nitrate reductase transcript levels in etiolated barley leaves

Cytokinin-controlled ribulose 1,5-bisphosphate carboxylase gene expression in pumpkin cotyledons

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