Role of Cytochrome P450 Hydroxylase in the Decreased Accumulation of Vitamin E in Muscle from Turkeys Compared to that from Chickens

Abstract

Turkeys and chickens reared to 5 weeks of age and fed diets with feedstuffs low in endogenous tocopherols were examined. Treatments included feed supplemented with RRR (natural source vitamin E) alpha tocopheryl acetate (AcT, 35 mg/kg feed) and all-racemic (synthetic vitamin E) AcT (10 and 58 mg/kg feed). Alpha tocopherol hydroxylase activity was greater in liver microsomes prepared from turkeys compared to that from chickens (p < 0.01). Alpha and gamma tocopherol metabolites were higher in turkey bile than in chicken when assessing the RRR AcT diet and the all-racemic AcT diet at 58 mg/kg feed (p < 0.01). Turkey cytochrome P450 2C29 was increased relative to its chicken ortholog on the basis of RNA-Seq transcript abundance (p < 0.001) and activity-based protein profiling (p < 0.01) of liver tissue. Alpha tocopherol concentrations in plasma, liver, and muscle from turkey were lower than the respective tissues from chicken (p < 0.05). Lipid oxidation was greater in turkey thigh than in chicken (p < 0.05). These results suggest that elevated tocopherol metabolism by cytochrome P450 hydroxylase(s) in turkeys contributes to the decreased accumulation of alpha tocopherol in turkey tissues compared to that of chickens.

Dietary Curcumin Alleviated Aflatoxin B1-Induced Acute Liver Damage in Ducks by Regulating NLRP3–Caspase-1 Signaling Pathways

“…The expression levels of genes related to cytochrome P450s in healthy individual are lower than those in specimens stimulated by exogenous chemicals [ 36 ]. Some studies showed that genes expression related to CYP450 in tissues was modulated by nutritional factors in turkeys and chicken and inhibited by polyphenols in humans [ 9 , 37 ]. The results of this study demonstrated that CYP450 protein content was significantly increased in injured liver after AFB1 administration; there was a significant decrease in CYP450 protein content in the T 500 + AFB1 group.…”

Role of Maillard Reaction Products as Antioxidants in Washed Cod and Washed Turkey Muscle Oxidized by Added Hemoglobin

“…Characterization of WCM and WTM: Total lipid content and 𝛼tocopherol in each washed muscle were analyzed as described previously. [22] Briefly, 10 g of muscle were homogenized with a PT 10-35 Polytron (Brinkmann Instruments, Westbury NY) in 20 and 10 volumes of a 2:1 mixture chloroform/methanol (v/v) respectively and filtered into a funnel. Fifteen volumes of 0.8% KCl were then added to the filtrate with shaking, and the separatory funnels were stored at 4 °C for 6 h to phase separation.…”

Color stability and lipid oxidation in pork sausage as affected by rosemary extract and phospholipase A 2 : A possible role for depletion of neutral lipid hydroperoxides

“…α‐Tocopherol was analyzed following the method of Perez et al., (2016). Lipids were dissolved in 99% hexanes/1% isopropanol (v/v) at 100 and 25 mg/mL.…”

“…Lipids were extracted following the method of Folch et al (1957) with modifications by Perez et al (2016). After extraction, half of the lipid was dissolved in chloroform, and the other half of the total lipid in chloroform was completely dried under nitrogen gas, weighed, and dissolved in 99% hexanes/1% isopropyl alcohol.…”