Role of cyclooxygenase (COX)-1 and COX-2 dereivatives in acceleration of the ulcer healing by growth factors

Konturek, Stanisław J.; Brzozowski, T.; Konturek, Peter C.; Schuppan, Dietmar; Śliwowski, Zbigniew; Kwiecień, Sławomir; Pajdo, Robert; Hahn, EG

doi:10.1016/s0167-0115(00)80127-5

Regulatory Peptides

2000

DOI: 10.1016/s0167-0115(00)80127-5

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Role of cyclooxygenase (COX)-1 and COX-2 dereivatives in acceleration of the ulcer healing by growth factors

Stanisław J. Konturek¹,

T. Brzozowski²,

Peter C. Konturek³

et al.

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Gastrin activates paracrine networks leading to induction of PAI-2 via MAZ and ASC-1

Almeida-Vega¹,

Catlow²,

Kenny³

et al. 2009

American Journal of Physiology-Gastrointestinal and Liver Physi

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The gastric hormone gastrin regulates the expression of a variety of genes involved in control of acid secretion and also in the growth and organization of the gastric mucosa. One putative target is plasminogen activator inhibitor-2 (PAI-2), which is a component of the urokinase activator system that acts extracellularly to inhibit urokinase plasminogen activator (uPA) and intracellularly to suppress apoptosis. Previous studies have demonstrated that gastrin induces PAI-2 both in gastric epithelial cells expressing the gastrin (CCK-2) receptor and, via activation of paracrine networks, in adjacent cells that do not express the receptor. We have now sought to identify the response element(s) in the PAI-2 promoter targeted by paracrine mediators initiated by gastrin. Mutational analysis identified two putative response elements in the PAI-2 promoter that were downstream of gastrin-activated paracrine signals. One was identified as a putative MAZ site, mutation of which dramatically reduced both basal and gastrin-stimulated responses of the PAI-2 promoter by a mechanism involving PGE(2) and the small GTPase RhoA. Yeast one-hybrid screening identified the other as binding the activating signal cointegrator-1 (ASC-1) complex, which was shown to be the target of IL-8 released by gastrin. RNA interference (RNAi) knockdown of two subunits of the ASC-1 complex (p50 and p65) inhibited induction of PAI-2 expression by gastrin. The data reveal previously unsuspected transcriptional mechanisms activated as a consequence of gastrin-triggered paracrine networks and emphasize the elaborate and complex cellular control mechanisms required for a key component of tissue responses to damage and infection.

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Gastrin activates paracrine networks leading to induction of PAI-2 via MAZ and ASC-1

Almeida-Vega¹,

Catlow²,

Kenny³

et al. 2009

American Journal of Physiology-Gastrointestinal and Liver Physi

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H(2)S-Releasing Aspirin Protects against Aspirin-Induced Gastric Injury via Reducing Oxidative Stress

et al. 2012

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The aim of this study was to examine the effect of ACS14, a hydrogen sulfide (H2S)-releasing derivative of aspirin (Asp), on Asp-induced gastric injury. Gastric hemorrhagic lesions were induced by intragastric administration of Asp (200 mg/kg, suspended in 0.5% carboxymethyl cellulose solutions) in a volume of 1 ml/100 g body weight. ACS14 (1, 5 or 10 mg/kg) was given 30 min before the Asp administration. The total area of gastric erosions, H2S concentration and oxidative stress in gastric tissues were measured three hours after administration of Asp. Treatment with Asp (200 mg/kg), but not ACS14 (430 mg/kg, at equimolar doses to 200 mg/kg Asp), for 3 h significantly increased gastric mucosal injury. The damage caused by Asp was reversed by ACS14 at 1–10 mg/kg in a concentration-dependent manner. ACS14 abrogated Asp-induced upregulation of COX-2 expression, but had no effect on the reduced PGE2 level. ACS14 reversed the decreased H2S concentrations and blood flow in the gastric tissue in Asp-treated rats. Moreover, ACS14 attenuated Asp-suppressed superoxide dismutase-1 (SOD-1) expression and GSH activity, suggesting that ACS14 may stimulate antioxidants in the gastric tissue. ACS14 also obviously inhibited Asp-induced upregulation of protein expression of oxidases including XOD, p47phox and p67phox. In conclusion, ACS14 protects Asp induced gastric mucosal injury by inhibiting oxidative stress in the gastric tissue.

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Role of cyclooxygenase (COX)-1 and COX-2 dereivatives in acceleration of the ulcer healing by growth factors

Cited by 2 publications

References 0 publications

Gastrin activates paracrine networks leading to induction of PAI-2 via MAZ and ASC-1

Gastrin activates paracrine networks leading to induction of PAI-2 via MAZ and ASC-1

H(2)S-Releasing Aspirin Protects against Aspirin-Induced Gastric Injury via Reducing Oxidative Stress

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