Role of cyclic 3′,5t́-AMP in the action of physiological secretagogues on the metabolism of rat pancreas in vitro☆

Action of cholecystokinin and cholinergic agents on calcium transport in isolated pancreatic acinar cells.

“…Although the sequence of morphologic changes attending pancreatic enzyme secretion has been characterized (4), the coincident sequence of biochemical events is poorly understood and the mechanism by which CCK and cholinergic agents initiate pancreatic secretion has not been characterized. Some investigators (5)(6)(7)(8)(9)(10) have suggested that stimulation of cellular accumulation of adenosine 3',5'-cyclic monophosphate (cyclic AMP) is involved in stimulation of pancreatic enzyme secretion, while others (11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22) have focused on changes in membrane transport of cations, in general, and of calcium in particular.…”

The effect of catecholamine analogues upon amylase secretion from the mouse parotid gland in vivo: relationship to changes in cyclic AMP and cyclic GMP levels

“…However, use of the last two criteria as evidence for a role involving cyclic AMP in mediating hormone action must be done with caution since it has been reported that methylxanthines might not always act by increasing cyclic AMP levels [4] . Further, Hilz and Tarnowski have observed that dbc-AMP and cyclic AMP can have divergent biological effects [ 51 Cyclic AMP has also been implicated in the release of amylase from the pancrease (see [6] ).…”

“…For example, the increased elevation of cyclic AMP levels in rat parotid pieces caused by epinephrine was inhibited eighty per cent by EGTA without a resulting inhibition of amylase release [ 71. Also, there is considerable evidence that cyclic AMP does not mediate the action of all of the secretagogues causing amylase release from the pancreas [6,8]. Finally, cholinergic agents have no effect on cyclic AMP levels in rabbit parotid slices [9], but do increase guanosine 3:5'-cyclic monopliospliate (cyclic GMP) levels in slices of rat submaxillary glands [IO] 218 and other tissues [ 1 I] This latter observation raises the possibility that cyclic GMP might mediate some of the actions of secretagogues which do not appear to involve cyclic AMP.…”

Hydrolysis of the Cyclic 3′:5′‐Monophosphates of Adenosine and Guanosine by Rat Pancreas

“…A 10-day adaptation to protein malnutritution tended to increase the activity of the low-Km cyclic AMP phosphodiesterase. 4. It was concluded that the rat pancreas was able to hydrolyse cyclic nucleotides a t physiological concentrations and that more than one enzyme was apparently involved.…”

“…The dibutyryl derivative of cyclic AMP stimulates protein secretion from isolated mouse [I] and rabbit [2,3] pancreas but not from rat [4,5] and cat [6] pancreas. I n response to pancreozymin stimulation, cyclic AMP levels do not increase in guinea-pig pancreas in vitro [7] while the rise observed in vivo in the cat [S] is not clearly associated with protein release.…”