Role of Conserved Non-Coding Regulatory Elements in LMW Glutenin Gene Expression

Juhász, Angéla; Makai, Szabolcs; Sebestyén, Endre; Tamás, László; Balázs, Ervin

doi:10.1371/journal.pone.0029501

Cited by 26 publications

(33 citation statements)

References 69 publications

(90 reference statements)

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“…For sequence characterisation, promoters longer than 700 bp were used. List of motifs generally related to prolamin gene promoters were used based on the results of Juhász and co-workers (Juhász et al 2011). The terminology of motifs and "boxes" is the same as in the above study.…”

Section: Cis-regulatory Module Detectionmentioning

confidence: 99%

“…The cDNA based measurement was carried out as described in our earlier reports (Juhász et al 2011;Makai et al 2014a). All together 31 datasets (11 cDNA libraries and 19 microarray datasets) were used.…”

Section: Co-expression Network Constructionmentioning

confidence: 99%

“…Their transcription is regulated by trans-acting factors associated with cisacting elements as well as epigenetic factors (Fauteux and Strömvik 2009;Kawakatsu and Takaiwa 2010;Juhász et al 2011;Wen et al 2012). Earlier studies suggested that transcription of gliadin and LMW glutenin genes (Gli-1,2 and Glu-3, respectively) were influenced by methylation and imprinting while expression of HMW GS coding Glu-1 genes is less dependent on these epigenetic factors (Wen et al 2012).…”

Section: Introductionmentioning

confidence: 99%

“…A closer analysis of the expression profiles of prolamin genes and their responses to abiotic stresses indicate the presence of different regulatory mechanisms for each prolamin protein family (DuPont et al 2006;Hurkman et al 2013). A model for different transcriptional regulation mechanisms have already been proposed which is based on the conserved non-coding elements of the LMW GS coding Glu-3 genes (Juhász et al 2011).…”

Section: Introductionmentioning

confidence: 99%

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Multiple elements controlling the expression of wheat high molecular weight glutenin paralogs

Makai

Éva

Tamás

et al. 2015

Funct Integr Genomics

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Analysis of gene expression data generated by high-throughput microarray transcript profiling experiments coupled with cis-regulatory elements enrichment study and cluster analysis can be used to define modular gene programs and regulatory networks. Unfortunately, the high molecular weight glutenin subunits of wheat (Triticum aestivum) are more similar than microarray data alone would allow to distinguish between the three homoeologous gene pairs. However, combining cDNA expression libraries with microarray data a co-expressional network was built that highlighted the hidden differences between these highly similar genes. Duplex clusters of cis-regulatory elements were used to focus the co-expressional network of transcription factors to the putative regulatory network of Glu-1 genes. The focused network helped to identify several modules of transcriptional gene programs in the endosperm. Many of these programs demonstrated a conserved temporal pattern across the studied genotypes, however few others showed variance. Based on this network, transient gene expression assays were performed with mutated promoters to inspect the control of tissue specificity. Results indicated that the interactions of the ABRE│CBF cluster with distal promoter regions may have a dual role in regulation by both recruiting the transcription complex as well as suppressing it in non-endosperm tissue. A putative model of regulation is discussed.

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Section: Cis-regulatory Module Detectionmentioning

confidence: 99%

Section: Co-expression Network Constructionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Multiple elements controlling the expression of wheat high molecular weight glutenin paralogs

Makai

Éva

Tamás

et al. 2015

Funct Integr Genomics

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“…Regulation of SSPs’ expression depends on the interactions between cis -acting elements and trans -activating TFs (Mena et al, 2002; Shewry and Halford, 2002; Juhász et al, 2011). A diverse array of cis -acting elements in the promoters of these proteins has been verified.…”

Section: Introductionmentioning

confidence: 99%

Functional Characterization of TaFUSCA3, a B3-Superfamily Transcription Factor Gene in the Wheat

et al. 2017

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The end-use quality of wheat, including its unique rheology and viscoelastic properties, is predominantly determined by the composition and concentration of gluten proteins. While, the mechanism regulating expression of the seed storage protein (SSP) genes and other related genes in wheat remains unclear. In this study, we report on the cloning and functional identification of TaFUSCA3, a B3-superfamily transcription factor (TF) gene in wheat. Sequence alignment indicated that wheat and barley FUSCA3 genes are highly conserved. Quantitative reverse-transcription (qRT)-PCR analysis showed that the transcript of TaFUSCA3 was accumulated mostly in the stamens and the endosperms of immature wheat seeds. Yeast-one-hybrid results proved that the full-length TaFUSCA3 and its C-terminal region had transcriptional activities. Yeast-two-hybrid and bimolecular fluorescence complementation assays indicated that TaFUSCA3 could activate the expression of the high molecular weight glutenin subunit gene Glu-1Bx7 and interact with the seed-specific bZIP protein TaSPA. DNA-protein-interaction enzyme-linked immunosorbent assay demonstrated that TaFUSCA3 specifically recognizes the RY-box of the Glu-1Bx7 promoter region. Transient expression results showed that TaFUSCA3 could trans-activate the Glu-1Bx7 promoter, which contains eight RY-box sequences. TaFUSCA3 was unable to activate the downstream transcription when the RY-box was fully mutated. TaFUSCA3 could activate the transcription of the At2S3 gene promoter in a complementation of loss-of-function experiment using the Arabidopsis thaliana line fus3-3, which is a FUSCA3 mutant, demonstrating the evolutionary conservation of the TaFUSCA3 gene. In conclusion, the wheat B3-type TF, TaFUSCA3, is functional conserved between monocot and dicot, and could regulate SSP gene expression by interacting specifically with TaSPA.

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Wheat Proteins

Juhász

Békés²,

Wrigley

2014

Applied Food Protein Chemistry

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Role of Conserved Non-Coding Regulatory Elements in LMW Glutenin Gene Expression

Cited by 26 publications

References 69 publications

Multiple elements controlling the expression of wheat high molecular weight glutenin paralogs

Multiple elements controlling the expression of wheat high molecular weight glutenin paralogs

Functional Characterization of TaFUSCA3, a B3-Superfamily Transcription Factor Gene in the Wheat

Wheat Proteins

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