Role of coenzyme Q and vitamin E on stallion semen motility evaluated both in frozen and cooled-stored semen

Rossi, Melissa; Falomo, Maria Elena; Mantovani, Roberto

doi:10.1080/1828051x.2016.1239511

Cited by 8 publications

(6 citation statements)

References 36 publications

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“…The CoQ10 is present at high levels in the spermatozoon as its reduced form, ubiquinol [32,33]. It has been successfully used for freezing stallion semen with contrasting results [34][35][36][37], whereas other authors showed that adding it to the horse-sperm freezing medium did not increase sperm motility [38]. In humans, CoQ10 has been used in the semen of oligospermic patients successfully reducing the number of reactive oxygen species and decreasing sperm damage during cryopreservation [39].…”

Section: Introductionmentioning

confidence: 99%

Effect of Honey, Coenzyme Q10, and β-Carotene/α-Tocopherol as Novel Additives in Rabbit-Sperm Cryopreservation Extender

Gardela

Ruiz-Conca

Palomares

et al. 2023

Animals

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The effectiveness of rabbit-sperm cryopreservation is still below average compared to other domestic species. After the sperm cryopreservation process, post-thawing parameters like motility and membrane integrity are significantly compromised. The use of new extender constituents is an approach that can be used to improve the effectiveness of cryopreservation. Accordingly, we used honey (1.25, 2.5, 5, and 10%), coenzyme Q10 (100 and 200 μM), and β-carotene/α-tocopherol (500 μM/620 μM and 250 μM/310 μM) as candidate components for rabbit-sperm extenders during cryopreservation. Ejaculates from commercial adult rabbit bucks (n = 5) were cryopreserved using conventional freezing. Several post-thawing sperm parameters were assessed, including total motility, membrane integrity, viability, nuclear membrane integrity, acrosome reaction, and mitochondrial membrane potential and activation. Additionally, we performed hormonal analyses of the seminal plasma. Moreover, we analyzed the post-thawing levels of a molecular marker of sperm quality, proAKAP4, which was used in rabbits for the first time. Our findings showed that the 2.5% honey supplementation increased the post-thawing sperm motility (13.75 ± 3.75%) compared to the greater concentrations employed. However, the post-thawing motility was negatively affected by the coenzyme Q10 (0%, in both groups) but was not affected by the β-carotene/α-tocopherol supplementation (22 ± 18.15%, and 11.67 ± 10.17%). In conclusion, the cryopreservation protocols of this study did not help to maintain the sperm parameters after thawing. Further studies are required to identify novel protocols to mitigate the damage caused to rabbit sperm during cryopreservation.

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Section: Introductionmentioning

confidence: 99%

Effect of Honey, Coenzyme Q10, and β-Carotene/α-Tocopherol as Novel Additives in Rabbit-Sperm Cryopreservation Extender

Gardela

Ruiz-Conca

Palomares

et al. 2023

Animals

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“…In sperm cells, the defence mechanism against lipid peroxidation also involves non-enzymatic molecules such as thioredoxin and glutathione, thiol-containing molecules, and vitamins E, C, and D ( 42 ). For human and several mammalian species sperm, the freezing medium has been supplemented with low-molecular-weight antioxidants to reduce oxidative stress ( 16 , 17 , 23 , 28 ).…”

Section: Introductionmentioning

confidence: 99%

Effect of extender supplementation with low-molecular-weight antioxidants on selected quality parameters of cryopreserved canine spermatozoa

Lecewicz

Strzeżek

Kordan

et al. 2018

Journal of Veterinary Research

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Introduction The addition of low-molecular-weight antioxidants during the freezing process improves post-thaw sperm quality. The high antioxidant potential of cryopreserved semen could have a positive effect on the motility, viability, and energy status of sperm cells and their ability to bind to the zona pellucida of oocytes. The aim of the study was to determine the effects of different concentrations and combinations of vitamins E and C in a semen extender on selected quality parameters of frozen-thawed canine spermatozoa.Material and Methods The experimental material was the semen of four mixed-breed dogs. Sperm viability (motility, plasma membrane integrity, and mitochondrial function) was examined at 0, 60, and 120 min in semen samples supplemented with the extender and in the controls.Results Combined supplementation with vitamins C + E at a concentration of 200 + 200 μM /1 × 109 spermatozoa had the most profound effect on total sperm motility, linear motility, and the percentage of spermatozoa with intact plasma membrane and active mitochondria.Conclusion The synergistic activity of vitamins E and C had a more beneficial influence on the quality of frozen–thawed sperm than these non-enzymatic antioxidants applied separately.

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“…Each ejaculates were divided into three treatments, being control (extender BotuCrio® -Botupharma, Botucatu, SP, Brazil) and the same extender with two different concentrations of CoQ-10 (Sigma-Aldrich® -C9538) that were added (50 µmol of CoQ-10 and 1 mmol of CoQ-10) (Figure 1). These two chosen concentrations of CoQ-10 were based on literature review, especially in papers (Lewin and Lavon, 1997;Rossi et al, 2016). Hence, the treatments were denominated as control (CONT), 50 µmol CoQ-10 and 1 mmol CoQ-10.…”

Section: Extender Treatmentsmentioning

confidence: 99%

Coenzyme Q-10 improves preservation of mitochondrial functionality and actin structure of cryopreserved stallion sperm

Lançoni

Celeghini

Giuli³

et al. 2021

Anim. Reprod.

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Coenzyme Q-10 (CoQ-10) is a cofactor for mitochondrial electron transport chain and may be an alternative to improve sperm quality of cryopreserved equine semen. This work aimed to improve stallion semen quality after freezing by adding CoQ-10 to the cryopreservation protocol. Seven saddle stallions were utilized. Each animal was submitted to five semen collections and freezing procedures. For cryopreservation, each ejaculate was divided in three treatments: 1) Botucrio® diluent (control); 2) 50 μmol CoQ-10 added to Botucrio® diluent; 3) 1 mmol CoQ-10 added to Botucrio® diluent. Semen batches were analyzed for sperm motility characteristics (CASA), plasma and acrosomal membranes integrity and mitochondrial membrane potential (by fluorescence probes propidium iodide, Hoechst 33342, FITC-PSA and JC-1, respectively), alterations in cytoskeletal actin (phalloidin-FITC) and mitochondrial function (diaminobenzidine; DAB). The 1 mmol CoQ-10 treatment presented higher (P<0.05) amount (66.8%) of sperm cells with fully stained midpiece (indicating high mitochondrial activity) and higher (P<0.05) amount (81.6%) of cells without actin reorganization to the post-acrosomal region compared to control group (60.8% and 76.0%, respectively). It was concluded that the addition of 1 mmol CoQ-10 to the freezing diluent was more effective in preserving mitochondria functionality and cytoskeleton of sperm cells submitted to cryopreservation process.

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Role of coenzyme Q and vitamin E on stallion semen motility evaluated both in frozen and cooled-stored semen

Cited by 8 publications

References 36 publications

Effect of Honey, Coenzyme Q10, and β-Carotene/α-Tocopherol as Novel Additives in Rabbit-Sperm Cryopreservation Extender

Effect of Honey, Coenzyme Q10, and β-Carotene/α-Tocopherol as Novel Additives in Rabbit-Sperm Cryopreservation Extender

Effect of extender supplementation with low-molecular-weight antioxidants on selected quality parameters of cryopreserved canine spermatozoa

Coenzyme Q-10 improves preservation of mitochondrial functionality and actin structure of cryopreserved stallion sperm

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