Role of CO2 pneumoperitoneum-induced acidosis in CO2 pneumoperitoneum-enhanced adhesion formation in mice

Effect of Reteplase™ and PAI-1 antibodies on postoperative adhesion formation in a laparoscopic mouse model

“…animals, anaesthesia and ventilation, laparoscopic surgery, induction and scoring of intraperitoneal adhesions, has been described in detail previously [2,3,[6][7][8][9][10][11][12][13][14][15] (Fig. 1).…”

“…1A, B) since adequate ventilation was found to be important, affecting adhesion formation [15]. Humidified air for ventilation was used in order to prevent cooling, as occurs during ventilation with nonhumidified air [8].…”

Carbon dioxide pneumoperitoneum, intraperitoneal pressure, and peritoneal tissue hypoxia: a mouse study with controlled respiratory support

“…It is therefore plausible that the high IPP of 15 mmHg in mice would decrease peritoneal blood flow caused by a mechanical effect, low cardiac output, or both [36,38], resulting in an extremely low PitO 2 . In addition, the high IPP of 15 mmHg contributes to the development of severe acidosis in mice [9], which can induce hypoxia. The current study demonstrated that few mesothelial cells were present in the peritoneal membrane in the 15-mmHg IPP group.…”

Application of stereology to study the effects of pneumoperitoneum on peritoneum

“…Wildbrett et al [21] demonstrated that insufflation with either CO 2 or He causes significant changes of intra-and extracellular parameters regulating essential cell functions such as oxidative phosphorylation to produce ATP, cell proliferation, and onset of apoptosis. Additionally, recent animal experiments have revealed that the laparoscopic peritoneal environment is hypoxic [22,23]. Hypoxia in tissues might exert the strongest effect on peritoneal cells, such as changing the microfilament-based cytoskeleton, and cellular ion and electrical potentials [24].…”