Role of chymase on growth of cultured canine Tenon's capsule fibroblasts and scarring in a canine conjunctival flap model

Maruichi, Midori; Sugiyama, Tetsuya; Ueki, Mari; Oku, Hidehiro; Sakaguchi, Masato; Okamoto, Yukiko; Muramatsu, Michiko; Ikeda, Tsunehiko; Miyazaki, Mizuo

doi:10.1016/j.exer.2004.02.009

Cited by 24 publications

(15 citation statements)

References 31 publications

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“…Chymaseactivated SCF may stimulate mast cell development and proliferation. Although we did not determine the mast cell number in the age-matched normal hamsters in the present study, we have shown that mast cell number was related to chymase activity in experimental models (22,23). Therefore, the increase of chymase activity might be dependent on the accumulation of mast cells after the STZ injection, and in contrast, the inhibitory effect of TY-51469 might contribute to the attenuation of mast cell accumulation.…”

Section: Discussionmentioning

confidence: 57%

Chymase Inhibition Provides Pancreatic Islet Protection in Hamsters With Streptozotocin-Induced Diabetes

et al. 2009

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Abstract. Angiotensin II may be involved in pancreatic disorganization, but the involvement of chymase has been unclear. In the present study, we examined whether chymase is involved in pancreatic disorganization in hamsters with streptozotocin (STZ)-induced diabetes. Hamsters were injected with streptozotocin (60 mg/kg), and non-injected hamsters served as controls. To investigate the effect of a chymase inhibitor, TY-51469 (30 mg/kg per day), hamsters in the STZ group were administered TY-51469 or placebo from 2 weeks after STZ injection, for 1 week. A significant increase in blood glucose level was observed at 1 week after STZ injection. This was maintained at 2 weeks, and a further significant increase was observed at 3 weeks. Until 2 weeks after STZ injection, all angiotensin II-related enzyme activities were unchanged, but at 3 weeks pancreatic chymase and total angiotensin II-forming activities, but not angiotensin-converting enzyme activity, were significantly increased. TY-51469 significantly attenuated blood glucose level along with reductions of chymase and total angiotensin II-forming activities and malondialdehyde level. Furthermore, there were significantly more pancreatic islets in the TY-51469 group than in the placebo group. In conclusion, chymase inhibition might protect against pancreatic islet disorganization.

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Section: Discussionmentioning

confidence: 57%

Chymase Inhibition Provides Pancreatic Islet Protection in Hamsters With Streptozotocin-Induced Diabetes

et al. 2009

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“…Chymase might play an important role in the accumulation of mast cells that express chymase through activating stem cell factor. In fact, in experimental models, chymase inhibition attenuates not only chymase activity but also mast cell accumulation 25,26) . Exogenous Ang infusion might induce aortic ACE and chymase in the aortae of apoE-deficient mice, and their increases might contribute to the progression of AAA.…”

Section: Discussionmentioning

confidence: 99%

“…The reason for this might be related to the functions of chymase that are in addition to its role in Ang -formation. Chymase is stored in mast cells; thus, the accumulation of mast cells results in increased chymase activity 25,26) . Chymase can proteolytically activate the stem cell factor that is essential for mast cell development and proliferation 27) .…”

Section: Discussionmentioning

confidence: 99%

Involvement of Vascular Angiotensin II-Forming Enzymes in the Progression of Aortic Abdominal Aneurysms in Angiotensin II- Infused ApoE-Deficient Mice

Inoue

Muramatsu

Jin

et al. 2009

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“…One of the most important functions of chymase is the regulation of angiotensin (Ang)II generation. In addition, chymase promotes myocardial and skin fibroblast proliferation (10,11), the release of transforming growth factor-β1 bound to the extracellular matrix and the degradation of procollagen protein that participate in tissue remodeling (12) and inflammation (13). Previous studies have shown that mast cell chymase is active during the healing process in burn wounds (14,15).…”

Section: Introductionmentioning

confidence: 99%

Expression and activity levels of chymase in mast cells of burn wound tissues increase during the healing process in a hamster model

Dong

et al. 2015

Experimental and Therapeutic Medicine

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Abstract. The present study aimed to investigate the changes in the expression levels and activity of mast cell chymase in the process of burn wound healing in a hamster model of deep second-degree burn. The hamster model was established by exposing a ~3 cm diameter area of bare skin to hot water (75˚C) for 0, 6, 8, 10 or 12 sec. Tissue specimens were collected 24 h after burning and histological analysis revealed that hot water contact for 12 sec was required to produce a deep second-degree burn. Quantitative polymerase chain reaction and a radioimmunoassay were used to the determine changes in chymase mRNA expression levels and activity. The mRNA expression levels and activity of chymase were increased in the burn wound tissues when compared with the normal skin. However, no statistically significant differences were observed in mast cell chymase activity amongst the various post-burn stages. Chymase mRNA expression levels peaked at day 1 post-burn, subsequently decreasing at days 3 and 7 post-burn and finally increasing again at day 14 post-burn. In summary, a hamster model of deep second-degree burn can be created by bringing the skin into contact with water at 75˚C for 12 sec. Furthermore, the mRNA expression levels and activity of chymase in the burn wound tissues increased when compared with those in normal skin tissues. IntroductionSkin wound healing is a complex multifactorial process, involving inflammation, cell proliferation, cell migration, re-epithelialization, angiogenesis, extracellular matrix deposition and remodeling (1,2). Burn wounds have a long healing period, and are the most representative persistent wounds for use in the study of skin wound healing. Deep second-degree burn wounds exhibit spontaneous epithelial regeneration through the proliferation and migration of the skin appendages under the wound and the epidermal cells around the wound margin (3). The healing period is relatively long; thus, deep second-degree burns are useful for the experimental study of burn wounds. Animal models of burn wounds have been used to study various pathological changes and healing mechanisms involved in the burn wound healing process (4).Burn-induced tissue ischemia and inflammation can increase the number of mast cells in burn tissues (5) and stimulate mast cells to release vasoactive substances by degranulation (6). Mast cells play a role in the acute inflammatory phase and participate in wound healing together with fibroblasts (7,8). Once activated, mast cells degranulate and release histamine, heparin and a variety of enzymes, such as chymase, cathepsin G and hydroxy peptidase A (9). Chymase is closely associated with tissue fibrosis.Chymase is an α-chymase-like serine protease that is involved in numerous physiological and pathophysiological processes. One of the most important functions of chymase is the regulation of angiotensin (Ang)II generation. In addition, chymase promotes myocardial and skin fibroblast proliferation (10,11), the release of transforming growth factor-β1 bound to the extracel...

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Role of chymase on growth of cultured canine Tenon's capsule fibroblasts and scarring in a canine conjunctival flap model

Cited by 24 publications

References 31 publications

Chymase Inhibition Provides Pancreatic Islet Protection in Hamsters With Streptozotocin-Induced Diabetes

Chymase Inhibition Provides Pancreatic Islet Protection in Hamsters With Streptozotocin-Induced Diabetes

Involvement of Vascular Angiotensin II-Forming Enzymes in the Progression of Aortic Abdominal Aneurysms in Angiotensin II- Infused ApoE-Deficient Mice

Expression and activity levels of chymase in mast cells of burn wound tissues increase during the healing process in a hamster model

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