Role of chromatography in the development of Standard Reference Materials for organic analysis

Wise, Stephen A.; Phinney, Karen W.; Sander, Lane C.; Schantz, Michele M.

doi:10.1016/j.chroma.2012.05.093

Cited by 24 publications

(14 citation statements)

References 94 publications

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“…The use of stationary phases with differing selectivity reduces the likelihood of measurement bias arising from undetected interferences. 36 Both PFP and CN columns were also utilized in the NIST LC-MS/MS measurements (see Table S-1, Supporting Information). In particular, a small interference precluded the use of the PFP column in quantification of 25(OH)D 3 in Level 2 of the SRM.…”

Section: Resultsmentioning

confidence: 99%

Development of an Improved Standard Reference Material for Vitamin D Metabolites in Human Serum

et al. 2017

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The National Institute of Standards and Technology (NIST) has developed Standard Reference Material (SRM) 972a Vitamin D Metabolites in Frozen Human Serum as a replacement for SRM 972, which is no longer available. SRM 972a was developed in collaboration with the National Institutes of Health’s Office of Dietary Supplements. In contrast to the previous reference material, three of the four levels of SRM 972a are comprised of unmodified human serum. This SRM has certified and reference values for the following 25-hydroxyvitamin D [25(OH)D] species: 25(OH)D2, 25(OH)D3, and 3-epi-25(OH)D3. The value assignment and certification process included three isotope-dilution mass spectrometry approaches, with measurements performed at NIST and at the Centers for Disease Control and Prevention (CDC). The value assignment methods employed have been modified from those utilized for the previous SRM, and all three approaches now incorporate chromatographic resolution of the stereoisomers, 25(OH)D3 and 3-epi-25(OH)D3.

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Section: Resultsmentioning

confidence: 99%

Development of an Improved Standard Reference Material for Vitamin D Metabolites in Human Serum

et al. 2017

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“…Therefore, one aspect of this study involved assessing the concordance of results from the two methods. The use of two or more independent analytical methods is a common approach to value assignment of reference materials at NIST because it reduces the likelihood of undetected measurement errors or bias (30, 31). …”

Section: Resultsmentioning

confidence: 99%

Value Assignment of Vitamin D Metabolites in Vitamin D Standardization Program Serum Samples

et al. 2017

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Assay variability has been cited as an obstacle to establishing optimal vitamin D exposure. As part of the Vitamin D Standardization Program (VDSP) effort to standardize the measurement of total 25(OH)D, value assignment of total 25(OH)D in 50 single donor serum samples was performed using two isotope-dilution LC-MS/MS methods. Both methods are recognized as reference measurement procedures (RMPs) by the Joint Committee for Traceability in Laboratory Medicine. These samples and their assigned values serve as the foundation for several aspects of the VDSP. To our knowledge, this is the first time that two RMPs have been used to assign 25(OH)D values to such a large number of serum samples.

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“…However, it was this goal that motivated and drove the development and improvement of LC and GC methods for PAHs at NIST for several decades (45,64). Several reviews have been published describing these challenges in the development of SRMs for PAHs and other environmental contaminants (45,64,(117)(118)(119).…”

Section: Srms From Nistmentioning

confidence: 98%

“…These isomeric fractions are then analyzed by reversed-phase LC-fluorescence to separate and quantify the individual PAH isomers. This multidimensional LC approach has been described in detail in several publications (19,(41)(42)(43) and review articles (28,44,45). The multidimensional LC approach is illustrated in Figure 11 for the analysis of a coal tar sample.…”

Section: Multidimensional Lc Approachmentioning

confidence: 99%

Analytical Methods for Determination of Polycyclic Aromatic Hydrocarbons (PAHs) — A Historical Perspective on the 16 U.S. EPA Priority Pollutant PAHs

Wise

Sander

Schantz

2015

Polycyclic Aromatic Compounds

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The identification of 16 polycyclic aromatic hydrocarbons (PAHs) as priority pollutants by the U.S. Environmental Protection Agency (EPA) in 1976 has been a primary driver for analytical methods development for the determination of PAHs. In this article, the historical development of methods in liquid chromatography (LC) and gas chromatography (GC) to separate these 16 PAHs is discussed. In LC a significant effort was the search for and the fundamental understanding of the unique stationary phase capable of achieving the desired separation of the 16 EPA PAHs. For GC methods, the focus on stationary phase development has been the separation of critical isomers with a broader scope than the 16 EPA PAHs. The current routine LC and GC methods for the 16 EPA PAHs are well established; however, new advances in analytical techniques beyond LC and GC are discussed. Many analysts are now interested in more than just the 16 EPA PAHs (e.g., higher molecular mass PAHs and alkyl-substituted PAHs) and analytical methods have emerged to address these needs. Reference materials and their use in the determination of PAHs are discussed.

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Role of chromatography in the development of Standard Reference Materials for organic analysis

Cited by 24 publications

References 94 publications

Development of an Improved Standard Reference Material for Vitamin D Metabolites in Human Serum

Development of an Improved Standard Reference Material for Vitamin D Metabolites in Human Serum

Value Assignment of Vitamin D Metabolites in Vitamin D Standardization Program Serum Samples

Analytical Methods for Determination of Polycyclic Aromatic Hydrocarbons (PAHs) — A Historical Perspective on the 16 U.S. EPA Priority Pollutant PAHs

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