Role of Cholesterol and Oxysterols in Regulating PTP1B Activity

Boivin, Benoît; Sagabala, Reddy Sudheer

doi:10.1016/j.freeradbiomed.2017.10.223

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“…Protein tyrosine phosphatase 1B (PTP1B) is a well-known regulator of the insulin and leptin signaling pathways and it has become an attractive therapeutic target for diabetes, obesity, and breast cancer [ 6 ]. Although the role of PTP1B in regulating cholesterol levels is unclear, a cholesterol-binding domain has previously been identified in the C-tail of PTP1B [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Cholesterol Chip for the Study of Cholesterol–Protein Interactions Using SPR

Faris

Sagabala

et al. 2022

Biosensors

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Cholesterol, an important lipid in animal membranes, binds to hydrophobic pockets within many soluble proteins, transport proteins and membrane bound proteins. The study of cholesterol–protein interactions in aqueous solutions is complicated by cholesterol’s low solubility and often requires organic co-solvents or surfactant additives. We report the synthesis of a biotinylated cholesterol and immobilization of this derivative on a streptavidin chip. Surface plasmon resonance (SPR) was then used to measure the kinetics of cholesterol interaction with cholesterol-binding proteins, hedgehog protein and tyrosine phosphatase 1B.

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Section: Introductionmentioning

confidence: 99%