Role of Central Metabolism in the Osmoadaptation of the Halophilic Bacterium Chromohalobacter salexigens

Pastor, José M.; Bernal, Vicente; Salvador, Manuel; Argandoña, Montserrat; Vargas, Carmen Regla; Csonka, László N.; Sevilla, A.; Iborra, José L.; Nieto, Joaquı́n J.; Cánovas, Manuel

doi:10.1074/jbc.m113.470567

Cited by 56 publications

(112 citation statements)

References 61 publications

(90 reference statements)

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“…It has been shown recently that the glucose catabolism in C . salexigens and other bacteria of the Halomonadaceae proceeds entirely via the ED-pathway [26, 42]. A possible explanation from these differences can be attributed to the specificity of the 6-phosphofructokinase present in H .…”

Section: Resultsmentioning

confidence: 99%

“…elongata and other halophilic bacteria that allow them to cope with salt concentrations well above 1.7 M NaCl (10%). In a recent paper, Pastor and coworkers analyzed the metabolism of the halophilic bacterium Chromohalobacter salexigens [26], which has a similar salt tolerance to H . elongata and synthesizes ectoine as its major compatible solute.…”

Section: Introductionmentioning

confidence: 99%

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Osmoregulation in the Halophilic Bacterium Halomonas elongata: A Case Study for Integrative Systems Biology

et al. 2017

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Halophilic bacteria use a variety of osmoregulatory methods, such as the accumulation of one or more compatible solutes. The wide diversity of compounds that can act as compatible solute complicates the task of understanding the different strategies that halophilic bacteria use to cope with salt. This is specially challenging when attempting to go beyond the pathway that produces a certain compatible solute towards an understanding of how the metabolic network as a whole addresses the problem. Metabolic reconstruction based on genomic data together with Flux Balance Analysis (FBA) is a promising tool to gain insight into this problem. However, as more of these reconstructions become available, it becomes clear that processes predicted by genome annotation may not reflect the processes that are active in vivo. As a case in point, E. coli is unable to grow aerobically on citrate in spite of having all the necessary genes to do it. It has also been shown that the realization of this genetic potential into an actual capability to metabolize citrate is an extremely unlikely event under normal evolutionary conditions. Moreover, many marine bacteria seem to have the same pathways to metabolize glucose but each species uses a different one. In this work, a metabolic network inferred from genomic annotation of the halophilic bacterium Halomonas elongata and proteomic profiling experiments are used as a starting point to motivate targeted experiments in order to find out some of the defining features of the osmoregulatory strategies of this bacterium. This new information is then used to refine the network in order to describe the actual capabilities of H. elongata, rather than its genetic potential.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Osmoregulation in the Halophilic Bacterium Halomonas elongata: A Case Study for Integrative Systems Biology

et al. 2017

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“…Intracellular accumulation of these solutes enables cells to maintain osmotic balance and avoid irreversible dehydration. These compounds may reach high concentrations without interference with cellular processes (Roessler and Müller, 2001;Pastor et al, 2013). Compatible solutes can be divided into several structural groups: sugars (trehalose, surcose), polyols (glicerol, sorbitol, mannitol, α-glucosyl-glycerol, mannosyl-glycerol, mannosyl-glyceramide), N-acetylated diamino acids (like N-acetylglutaminylglutamine amide), betaines (such as glycine betaine and derivatives), amino acids (proline, glutamate, glutamine, alanine, ectoine and hydroxyectoine) and derivatives (Yancey, 2005).…”

Section: Introductionmentioning

confidence: 99%

Protective effects of ectoine on behavioral, physiological and biochemical parameters of Daphnia magna subjected to hydrogen peroxide

Bownik¹,

Stępniewska²

2015

Comparative Biochemistry and Physiology Part C: Toxicology &

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“…Some studies indicated that CO may be lethal to fish if used at excessive concentrations or if regulations about time of aneshesia specific for a single fish species are not followed (Sladky et al, 2001).This compound also causes various toxic effects in human colon cancer cell lines in the in vitro conditions and in insects, therefore it has been used as an insecticide (US EPA, 2004;Boyer et al, 2009).The potential harmful effects of CO on nontarget aquatic animals such as invertebrates was also reported. For example, a reduction growth and bleaching of corals was observed after repeated field application of the oil (Pastor et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Untitled

Bownik¹

2016

Turk. J. Fish. Aquat. Sci.

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Clove oil (CO) used as an anaesthetic in aquaculture causes toxic effects in fish and invertebrates since it has a narrow margin between effective and lethal concentrations. Ectoine (ECT) is an osmoprotectant produced by halophilic bacteria in response to stressful factors. It was hypothesized that ECT may attenuate physiological alterations induced by CO in aquatic animals. The aim of the present study was to determine if ECT induces protective effects in Daphnia magna subjected to COinduced anaesthesia. Daphnids were treated with 50 or 150 µL/L CO alone or in the combination with 10, 25 or 50 mg/L ECT. The results showed that both concentrations of CO alone induced anaesthesia, heart arrhythmia, decreased heart rate, increased diastole/systole ratio and duration of diastole. On the other hand, ECT attenuated alterations evoked by CO with the most significant effects in the combination of 50 µL/L CO+25 mg/L ECT. The animals showed a prolonged TtA and attenuated depression of thoracic limb activity, heart rate, arrhythmia, decreased diastole/systole ratio and duration of diastole.The results indicate that ECT may be a cardioprotective agent in anaesthesized crustaceans.

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Role of Central Metabolism in the Osmoadaptation of the Halophilic Bacterium Chromohalobacter salexigens

Cited by 56 publications

References 61 publications

Osmoregulation in the Halophilic Bacterium Halomonas elongata: A Case Study for Integrative Systems Biology

Osmoregulation in the Halophilic Bacterium Halomonas elongata: A Case Study for Integrative Systems Biology

Protective effects of ectoine on behavioral, physiological and biochemical parameters of Daphnia magna subjected to hydrogen peroxide

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