Role of cell wall in freezing tolerance of cultured potato cells and their protoplasts

Tao, Dali; Li, P. H.; Carter, J. V.

doi:10.1111/j.1399-3054.1983.tb05738.x

Cited by 50 publications

(24 citation statements)

References 15 publications

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“…During incubation in LS solution for 20 min, meristematic cells became plasmolyzed, producing concentrated spherical protoplasts (Matsumoto et al 1998;Sakai et al 2002). Plasmolysis might mitigate the mechanical stress incurred during severe dehydration (Hellergren and Li 1981;Tao et al 1983;Jitsuyama et al 1997). A higher concentration of sucrose (1.6 M) in LS solution and a longer period of osmoprotection were necessary to increase the osmotolerance of sweet potato Fig.…”

Section: Resultsmentioning

confidence: 97%

Simplified cryopreservation of sweet potato [Ipomoea batatas (L.) Lam.] by optimizing conditions for osmoprotection

Hirai¹,

Sakai²

2003

Plant Cell Reports

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Shoot tips of sweet potato were successfully cryopreserved using an encapsulation vitrification method. Encapsulated shoot tips were pre-incubated in liquid Murashige-Skoog medium containing 30 g/l sucrose for 24 h, then precultured in sucrose-enriched medium (0.3 M sucrose) for 16 h. Shoot tips were osmoprotected with a mixture of 2 M glycerol and 1.6 M sucrose for 3 h before being dehydrated with a highly concentrated vitrification solution (PVS2) for 1 h at 25 degrees C. The encapsulated and dehydrated shoot tips were transferred to a 2 ml cryotube, suspended in 0.5 ml PVS2, and plunged directly into liquid nitrogen. Rapidly warmed shoot tips developed normal shoots and roots in 21 days without any morphological abnormalities after plating on a recovery medium. High levels (average of about 80%) of shoot formation were obtained for three cultivars of sweet potato. This encapsulation vitrification method appears promising for cryopreservation of sweet potato germplasm.

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Section: Resultsmentioning

confidence: 97%

Simplified cryopreservation of sweet potato [Ipomoea batatas (L.) Lam.] by optimizing conditions for osmoprotection

Hirai¹,

Sakai²

2003

Plant Cell Reports

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“…Ultrastructural analyses revealed considerable cellular damage in certain areas with total breakdown of protoplasts and sometimes cell wall rupture (Grout and Henshaw 1980). The influence of the cell wall in freezing tolerance of cultured potato cells was analysed by Tao et al (1983). In this study, the cell wall of cultured potato cells accounted for freezing injury because of mechanical stress between cell wall and plasma membrane during a freeze-thaw cycle.…”

Section: Ultrastructurementioning

confidence: 94%

Potato Shoot Tip Cryopreservation. A Review

2010

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Potato is one of the most important crops worldwide. Genetic resources of potato (Solanum tuberosum L. ssp. tuberosum) and related cultivated species are conserved through storage of tubers, in vitro plants and in cryopreservation. Cryopreservation, storage in or above liquid nitrogen, is the best option to maintain vegetatively propagated plants in the long term. The present review gives comprehensive information about various cryopreservation techniques for potato published from 1977 until the present. It discusses factors that affect the process and success of cryopreservation, such as donor culture conditions, preculture, cooling, warming and post-culture treatments. Studies are presented that analyse the histological and ultrastructural changes after different cryopreservation steps and the morphological pathways during regeneration of plants after rewarming. The maintenance of genetic stability in potato after cryopreservation has also been demonstrated by various phenotypic and molecular methods. The first thermal analyses on potato shoot tips are presented using differential scanning calorimetry to analyse the state of water during cooling and warming. Biochemical analyses of different compounds, such as soluble sugars and proteins, have been performed to understand and improve existing cryogenic methods. Potato is an example where successful virus elimination has been obtained via cryopreservation of shoot tips (cryotherapy). There are already cryopreserved collections of potato shoot tips in

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“…It has been a matter of discussion whether the cell wall contributes to the freezing tolerance of a cell (2) or whether it even enhances the stress put on a cell by extracellular freezing (25). As far as negative pressures develop and thereby reduce freeze-dehydration, a positive effect of the cell wall is obvious.…”

Section: Negative 'P: Limitations and Physiological Significancementioning

confidence: 99%

Water Relations of Pachysandra Leaves during Freezing and Thawing

Jian-jun

Beck²

1991

Plant Physiol.

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The evergreen herb Pachysandra terminalis becomes moderately frost-hardy in winter. The water relations of its frost-hardy leaves were studied during a freeze-thaw cycle. Leaf water potentials, measured by psychrometry at subfreezing temperatures, were identical with those of ice, indicating equilibrium freezing. Microscopic observations showed extracellular freezing of tissue water. As evidenced by thermal analysis, the freezing process starts with the crystallization of a minor volume which was identified as apoplasmic water. The following long-lasting exotherm indicated slow export of water from the protoplasts driven by extracellular crystallization. In partially frozen leaves, the fractions of liquid water were measured at several subfreezing temperatures by nuclear magnetic resonance spectroscopy. They were consistently greater than those calculated from the osmotic potentials of cellular fluid, and the differences increased with decreasing temperature. About 50% of the differences could be abolished by freeze-killing of the leaf and was thus ascribed to the effect of a (negative) pressure reinforcing the osmotic potential. The persistent part of the differences may have reflected a matric component. At -70C, the absolute values of both potentials were -1.7 megapascals each. The water relations of Pachysandra leaves clearly indicate nonideal equilibrium freezing where negative pressures and matric potentials contribute to the leaf water potential and thus alleviate freeze-dehydration of the tissue.

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Role of cell wall in freezing tolerance of cultured potato cells and their protoplasts

Cited by 50 publications

References 15 publications

Simplified cryopreservation of sweet potato [Ipomoea batatas (L.) Lam.] by optimizing conditions for osmoprotection

Simplified cryopreservation of sweet potato [Ipomoea batatas (L.) Lam.] by optimizing conditions for osmoprotection

Potato Shoot Tip Cryopreservation. A Review

Water Relations of Pachysandra Leaves during Freezing and Thawing

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