Role of cell wall degrading enzymes in the interaction of poplar and Melampsora larici-populina Kleb.

Abstract

The activity of cell wall-degrading enzymes, produced in poplar cultivars infected Melampsora laricipopulina Kleb., was studied. The results show that PMG, PMTE, C X and β-glucosidase played roles during the infection. After inoculation, the activity of PMG in both susceptible and resistant cultivars had two peak values in 2 dpi and 5 dpi. The activities of PMTE and β-glucosidase had a peak value in 3 dpi, and C X in 2 dpi. Among these cell wall-degrading enzymes, the activities of PMG and PMTE were higher and the activities of C X and β-glucosidase were relatively lower. The activities of these cell wall-degrading enzymes were significantly higher in susceptible cultivars than those in resistant cultivars. All these demonstrated that these cell wall-degrading enzymes played certain roles in the infection of M. larici-populina.

Functional characterization of Melampsora larici‐populina Hog1‐type MAPK in response to osmotic stress

“…Poplar leaf rust disease caused by Melampsora larici‐populina results in threats to poplar plantations worldwide, including plantations in many regions of China (Pinon & Frey, 2005; Tian et al, 2000). Using resistant poplar cultivars is the most effective and environmentally friendly approach to control this disease.…”

“…Using resistant poplar cultivars is the most effective and environmentally friendly approach to control this disease. However, similar to other rust diseases, it is difficult to control because rapidly evolved races lead to the breakdown of poplar resistance (Tian et al, 2000). Our ability to control poplar leaf rust disease will benefit from understanding the molecular mechanism of development and pathogenesis.…”

“…Poplar leaf rust disease caused by Melampsora larici-populina results in threats to poplar plantations worldwide, including plantations in many regions of China (Pinon & Frey, 2005;Tian et al, 2000).…”

Phomopsis longanae Chi-Induced Changes in Activities of Cell Wall-Degrading Enzymes and Contents of Cell Wall Components in Pericarp of Harvested Longan Fruit and Its Relation to Disease Development

“…In botanic cells, the cell wall is the first barrier against the infection by fungal pathogens ( Kubicek et al, 2014 ). Cell wall-degrading enzymes (CWDEs) secreted by pathogens play a key role in penetrating the cell wall to utilize the nutrients ( Kang and Buchenauer, 2000 ; Lalaoui et al, 2000 ; Kikot et al, 2009 ; Tian et al, 2009 ; Gharbi et al, 2015 ; Ramos et al, 2016 ). There is a diverse array of CWDEs, including polygalacturonase (PG), pectin methylgalacturonase (PMG), pectinesterase (PE), pectin lyase (PL), pectate lyase (PNL), cellulase (C X ), β-glucosidase, and xylanases ( Al-Hindi et al, 2011 ; Li et al, 2012 ; Kubicek et al, 2014 ).…”