Role of cell surface hydrophobicity in Candida albicans biofilm

Bujdáková, Helena; Didiášová, Miroslava; Drahovská, Hana; Černáková, Lucia

doi:10.2478/s11535-013-0136-y

Cited by 28 publications

(25 citation statements)

References 11 publications

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“…The CSH1 gene product (Csh1), is a vital virulence factor in C. albicans , which affects cell surface hydrophobicity ( Antley and Hazen 1988 ). It is also involved in the irreversible adhesion of cells during the early biofilm formation ( Chandra et al , 2001 ; Bujdáková et al , 2013 ). Gca1 is a plasma membrane-associated glucoamylase enzyme with a role in the formation of C. albicans biofilm matrix ( Nobile et al , 2009 ) and has been reported to assist with starch degradation and energy generation ( Maicas et al , 2016 ).…”

Section: Resultsmentioning

confidence: 99%

Transcriptome Analyses of Candida albicans Biofilms, Exposed to Arachidonic Acid and Fluconazole, Indicates Potential Drug Targets

Kuloyo¹,

Fourie²,

Cason³

et al. 2020

G3 Genes|Genomes|Genetics

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Candida albicans is an opportunistic yeast pathogen within the human microbiota with significant medical importance because of its pathogenic potential. The yeast produces highly resistant biofilms, which are crucial for maintaining infections. Though antifungals are available, their effectiveness is dwindling due to resistance. Alternate options that comprise the combination of existing azoles and polyunsaturated fatty acids, such as arachidonic acid (AA), have been shown to increase azoles susceptibility of C. albicans biofilms; however, the mechanisms are still unknown. Therefore, transcriptome analysis was conducted on biofilms exposed to sub-inhibitory concentrations of AA alone, fluconazole alone, and AA combined with fluconazole to understand the possible mechanism involved with the phenomenon. Protein ANalysis THrough Evolutionary Relationships (PANTHER) analysis from the differentially expressed genes revealed that the combination of AA and fluconazole influence biological processes associated with essential processes including methionine synthesis processes and those involved in ATP generation, such as AMP biosynthesis, fumarate metabolism and fatty acid oxidation. These observation suggests that the interference of AA with these processes may be possible mechanisms to induce increased antifungal susceptibility

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Section: Resultsmentioning

confidence: 99%

Transcriptome Analyses of Candida albicans Biofilms, Exposed to Arachidonic Acid and Fluconazole, Indicates Potential Drug Targets

Kuloyo¹,

Fourie²,

Cason³

et al. 2020

G3 Genes|Genomes|Genetics

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“…Di Bonaventura et al [ 35 ] reported that biofilm formation in Listeria is significantly influenced by bacterial surface hydrophobicity. Bujdáková et al [ 36 ] reported that the gene responsible for cell surface hydrophobicity is upregulated during the formation of a sessile biofilm in comparison to that of the planktonic culture.…”

Section: Resultsmentioning

confidence: 99%

Effects of Fine Particulate Matter onPseudomonas aeruginosaAdhesion and Biofilm FormationIn Vitro

Woo

Lee

Park

et al. 2018

BioMed Research International

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Respiratory infections of Pseudomonas aeruginosa are a major cause of mortality and morbidity for hospitalized patients. Fine particulate matter (FPM) is known to have interactions with some bacterial infection in the respiratory system. In this report, we investigate the effect of different concentration of FPM on P. aeruginosa attachment and biofilm formation using in vitro cell culture systems. P. aeruginosa were cultured to form mature biofilms on hydroxyapatite-coated peg and the number of bacteria in the biofilms was enumerated. Morphology of biofilm was imaged with scanning electron microscopy and confocal laser scanning microscopy. Bacterial affinity change to the cell membrane was evaluated with attached colony counting and fluorescence microscopy images. Alteration of bacterial surface hydrophobicity and S100A4 protein concentration were explored as mechanisms of P. aeruginosa adhesion to human cells. There were a concentration-dependent increase of thickness and surface roughness of biofilm mass. P. aeruginosa adherence to respiratory epithelial cells was increased after FPM treatment. Bacterial surface hydrophobicity and S1000A4 protein concentration were increased with proportionally the dose of FPM in media. FPM in the airway could enhance both the adhesion of P. aeruginosa to epithelial cells and biofilm formation. Bacterial surface hydrophobicity and human cell plasma membrane injury are associated with binding of P. aeruginosa on airway epithelial cells and biofilm formation.

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“…One of the major factors contributing to the virulence of Candida spp. is their ability to grow in microbial community known as biofilm which displays unique phenotype compared to free‐living (planktonic) cells . A biofilm confers a significantly reduced susceptibility to antimicrobial compounds resulting from the expression of conventional resistance mechanisms, reduced penetration through the extracellular matrix surrounding the biofilm, or the activation of stress‐response mechanisms .…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of the Photoactive Dye Methylene Blue versus Caspofungin on the Candida parapsilosis Biofilm in vitro and ex vivo

Černáková

Chupáčová

Židlíková

et al. 2015

Photochem & Photobiology

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This research studied the effectiveness of the photoactive compound methylene blue (MB) activated with red LED light (576-672 nm) compared to that of caspofungin (CAS) on 1 Candida albicans and 3 Candida parapsilosis strains. Results were evaluated in terms of SMIC50 for CAS or in PDI (photodynamic inactivation)-SMIC50 for MB (minimal inhibitory concentration inhibiting sessile biofilm to 50% in comparison to the control without CAS or after irradiation in comparison to the control without MB). While all strains were susceptible to CAS in planktonic form, the SMIC50 was determined to be >16 μg mL(-1) when CAS was added to a 24 h biofilm. However, PDI-MIC50s (1.67 mW cm(-2) , fluence 15 J cm(-2) ) were 0.0075-0.03 mmol L(-1) . For biofilm, PDI-SMIC50s were in the range from 0.7 to 1.35 mmol L(-1) . MB concentration of 1 mmol L(-1) prevented a biofilm being formed ex vivo on mouse tongues after irradiation regardless of the application time, in contrast to CAS, which was only effective at a concentration of 16 μg mL(-1) when it was added at the beginning of biofilm formation. PDI seems to be a promising method for the prevention of microbial biofilms that do not respond significantly to conventional drugs.

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Role of cell surface hydrophobicity in Candida albicans biofilm

Cited by 28 publications

References 11 publications

Transcriptome Analyses of Candida albicans Biofilms, Exposed to Arachidonic Acid and Fluconazole, Indicates Potential Drug Targets

Transcriptome Analyses of Candida albicans Biofilms, Exposed to Arachidonic Acid and Fluconazole, Indicates Potential Drug Targets

Effects of Fine Particulate Matter onPseudomonas aeruginosaAdhesion and Biofilm FormationIn Vitro

Effectiveness of the Photoactive Dye Methylene Blue versus Caspofungin on the Candida parapsilosis Biofilm in vitro and ex vivo

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