Role of cell surface GM3 ganglioside and sialic acid in the antitumor activity of a GM3-based vaccine in the murine B16 melanoma model

Gabri, Mariano Rolando; Ripoll, Giselle Vanina; Alonso, Daniel F.; Gomez, Daniel E.

doi:10.1007/s00432-002-0385-7

Cited by 8 publications

(1 citation statement)

References 28 publications

(31 reference statements)

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“…In fact, antibodies reactive to NeuGc-containing glycolipids have been found to be induced in patients after repeated transfusions with sera from other species in melanoma patients [36]. Melanoma cells over-express GM3, and natural anti-NeuGc antibodies are increased in melanoma patients [37].…”

Section: Ganglioside Gm3 Based Cancer Vaccinesmentioning

confidence: 99%

Ganglioside GM3 and Its Role in Cancer

Zheng

Terreni

Sollogoub

et al. 2019

CMC

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Ganglioside GM3 is strongly related with human tumors, such as lung, brain cancers and melanomas, and more and more evidences have revealed that GM3 possesses powerful effects on cancer development and progression. GM3 is over expressed on several types of cancers, and can be as a tumor-associated carbohydrate antigen, used for immunotherapy of cancers. GM3 can also inhibit tumor cells growth by anti-angiogenesis or motility and so on. Especially, GM3 has effects on the EGFR tyrosine kinase signaling, uPAR-related signaling and glycolipid-enriched microdomains, which are essential for cancer signaling conduction. It is obvious that GM3 will be a promising target for cancer treatment.

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Section: Ganglioside Gm3 Based Cancer Vaccinesmentioning

confidence: 99%

Ganglioside GM3 and Its Role in Cancer

Zheng

Terreni

Sollogoub

et al. 2019

CMC

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Cancer‐Associated and Related Glycosphingolipid Antigens

Levery

2008

Carbohydrate‐Based Vaccines and Immunotherapies

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Immunization with a GM3 ganglioside nanoparticulated vaccine confers an effector CD8+ T cells-mediated protection against melanoma B16 challenge

Mazorra

Mesa

Fernández

et al. 2008

Cancer Immunol Immunother

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Preventive immunotherapy is an attractive strategy for patients at a high risk of having cancer. The success of prophylactic cancer vaccines would depend on the selection of target antigens that are essential for tumour growth and progression. The overexpression of GM3 ganglioside in murine and human melanomas and its important role in tumour progression makes this self antigen a potential target for preventive immunotherapy of this neoplasm. We have previously shown that preventive administration of a GM3-based vaccine to C57BL/6 mice elicited the rejection of the GM3 positive-B16 melanoma cells in most of the animals. Despite the crucial role of cellular immune response in tumour protection, the involvement of T cells in anti-tumour immunity of ganglioside vaccines is not described. Here, we examined the mechanisms by which this immunogen confers tumour protection. We have found that induction of anti-GM3 IgG antibodies correlated with tumour protection. Surprisingly, CD8(+) T cells, but not NK1.1(+) cells, are required in the effector phase of the antitumour immune response. The depletion of CD4(+) T cells during immunization phase did not affect the anti-tumour activity. In addition, T cells from surviving-immunized animals secreted IFNgamma when were co-cultured with IFNalpha-treated B16 melanoma cells or DCs pulsed with melanoma extract. Paradoxically, in spite of the glycolipidic nature of this antigen, these findings demonstrate the direct involvement of the cellular immune response in the anti-tumour protection induced by a ganglioside-based vaccine.

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Role of cell surface GM3 ganglioside and sialic acid in the antitumor activity of a GM3-based vaccine in the murine B16 melanoma model

Abstract: These results indicate that the antitumor activity of GM3/VSSP is associated with GM3 expression on tumor cell surface and demonstrate a major role of sialic acid in the humoral response of vaccinated mice.

Cited by 8 publications

References 28 publications

Ganglioside GM3 and Its Role in Cancer

Ganglioside GM3 and Its Role in Cancer

Cancer‐Associated and Related Glycosphingolipid Antigens

Immunization with a GM3 ganglioside nanoparticulated vaccine confers an effector CD8+ T cells-mediated protection against melanoma B16 challenge

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