Role of CD4+ and CD8α+ T cells in protective immunity against Edwardsiella tarda infection of ginbuna crucian carp, Carassius auratus langsdorfii

Yamasaki, Masatoshi; Araki, Kyosuke; Nakanishi, Teruyuki; Nakayasu, Chihaya; Yamamoto, Atsushi

doi:10.1016/j.fsi.2013.11.016

Cited by 50 publications

(22 citation statements)

References 51 publications

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“…In the context of the evolution of Th responses it is known that in bony fish CD4-1 + cells can express cytokines relevant to adaptive immunity after stimulation with mitogens and PAMPs [28] and that CD4-1 + cells can undergo proliferation when stimulated with specific antigen in vitro [27, 57]. However, no specific induction of cytokine expression has been reported in CD4-1 + (or CD4-2 + ) cells following antigen exposure, and hence it is unknown whether Th equivalents exist in fish despite evidence for their role in protective responses [29, 30]. This is critical to the discussion of when different Th subpopulations evolved and whether it is possible to manipulate Th subpopulations in fish during vaccination, as a desirable means to improve fish health in aquaculture.…”

Section: Discussionmentioning

confidence: 99%

“…Stimulation of the isolated cells with PAMPs or a T cell mitogen (ConA) in vitro elicited expression of several cytokines of adaptive immunity. More recently, two studies in ginbuna crucian carp show that CD4-1 + cells play a role in protection against bacterial and viral pathogens, using adoptive transfer of MACS sorted cells from sensitized syngeneic fish [29, 30]. In the case of CD4-2 + cells, one study has shown that when co-expressed with CD25 the CD4-2 + cells have an apparent Treg phenotype [26].…”

Section: Introductionmentioning

confidence: 99%

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First Demonstration of Antigen Induced Cytokine Expression by CD4-1+ Lymphocytes in a Poikilotherm: Studies in Zebrafish (Danio rerio)

et al. 2015

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Adaptive immunity in homeotherms depends greatly on CD4+ Th cells which release cytokines in response to specific antigen stimulation. Whilst bony fish and poikilothermic tetrapods possess cells that express TcR and CD4-related genes (that exist in two forms in teleost fish; termed CD4-1 and CD4-2), to date there is no unequivocal demonstration that cells equivalent to Th exist. Thus, in this study we determined whether CD4-1+ lymphocytes can express cytokines typical of Th cells following antigen specific stimulation, using the zebrafish (Danio rerio). Initially, we analyzed the CD4 locus in zebrafish and found three CD4 homologues, a CD4-1 molecule and two CD4-2 molecules. The zfCD4-1 and zfCD4-2 transcripts were detected in immune organs and were most highly expressed in lymphocytes. A polyclonal antibody to zfCD4-1 was developed and used with an antibody to ZAP70 and revealed double positive cells by immunohistochemistry, and in the Mycobacterium marinum disease model CD4-1+ cells were apparent surrounding the granulomas typical of the infection. Next a prime-boost experiment, using human gamma globulin as antigen, was performed and revealed for the first time in fish that zfCD4-1+ lymphocytes increase the expression of cytokines and master transcription factors relevant to Th1/Th2-type responses as a consequence of boosting with specific antigen.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

First Demonstration of Antigen Induced Cytokine Expression by CD4-1+ Lymphocytes in a Poikilotherm: Studies in Zebrafish (Danio rerio)

et al. 2015

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“…Recently, with the production of specific antibodies for fish T lymphocytes, it has become possible to investigate cellular immune responses at the translational level. This has been exemplified by the investigation of cellular immune responses induced by various antigens in carp and rainbow trout (54)(55)(56)(57)(58)(59). In this study, we used FCM analysis of single stained CD4-1 + , CD4-2 + , and CD8β + cells to evaluate the cellular immune responses induced by the pVAA vaccine.…”

Section: Discussionmentioning

confidence: 99%

A DNA Vaccine Encoding the VAA Gene of Vibrio anguillarum Induces a Protective Immune Response in Flounder

Xing

Tang

et al. 2019

Front. Immunol.

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Vibrio anguillarum is a pathogenic bacterium that infects flounder resulting in significant losses in the aquaculture industry. The VAA protein previously identified in flounder is associated with a role in immune protection within these fish. In the present study, a recombinant DNA plasmid encoding the VAA gene of V. anguillarum was constructed and its potential as a DNA vaccine, to prevent the infection of V. anguillarum in flounder fish, investigated. We verified the expression of the VAA protein both in vitro in cell lines and in vivo in flounder fish. The protective effects of pcDNA3.1-VAA (pVAA) were analyzed by determination of the percentage of sIgM+, CD4-1+, CD4-2+, CD8β+ lymphocytes, and the production of VAA-specific antibodies in flounder following their immunization with the DNA vaccine. Histopathological changes in immune related tissues, bacterial load, and relative percentage survival rates of flounder post-challenge with V. anguillarum, were all investigated to assess the efficacy of the pVAA DNA vaccine candidate. Fish intramuscularly immunized with pVAA showed a significant increase in CD4-1+, CD4-2+, and CD8β+ T lymphocytes at days 9, 11, and 14 post-vaccination, reaching peak T-cell levels at days 11 or 14 post-immunization. The percentage of sIgM+ lymphocytes reached peak levels at weeks 4–5 post-immunization. Specific anti-V. anguillarum or anti-rVAA antibodies were induced in inoculated fish at days 28–35 post-immunization. The liver of vaccinated flounder exhibited only slight histopathological changes compared with a significant pathology observed in control immunized fish. Additionally, a lower bacterial burden in the liver, spleen, and kidney were observed in pVAA protected fish in response to bacterial challenge, compared with pcDNA3.1 vector control injected fish. Moreover, the pVAA vaccine confers a relative percentage survival of 50.00% following V. anguillarum infection. In summary, this is the first study indicating an initial induction of the T lymphocyte response, followed by B lymphocyte induction of specific antibodies as a result of DNA immunization of flounder. This signifies the important potential of pVAA as a DNA vaccine candidate for the control of V. anguillarum infection.

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“…Yamasaki et al [ 89 ] showed the important role of cell-mediated immunity (CMI) in protection against E. tarda infection by adoptive transfer of sensitized lymphocytes. They adoptively transferred T-cell subsets sensitized with E. tarda to isogenic naïve ginbuna to identify the T-cell subsets involved in protecting fish from infection.…”

Section: Function Of Fish T Cellsmentioning

confidence: 99%

T Cells in Fish

Nakanishi

Shibasaki

Matsuura

2015

Biology

Self Cite

129

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Cartilaginous and bony fish are the most primitive vertebrates with a thymus, and possess T cells equivalent to those in mammals. There are a number of studies in fish demonstrating that the thymus is the essential organ for development of T lymphocytes from early thymocyte progenitors to functionally competent T cells. A high number of T cells in the intestine and gills has been reported in several fish species. Involvement of CD4+ and CD8α+ T cells in allograft rejection and graft-versus-host reaction (GVHR) has been demonstrated using monoclonal antibodies. Conservation of CD4+ helper T cell functions among teleost fishes has been suggested in a number studies employing mixed leukocyte culture (MLC) and hapten/carrier effect. Alloantigen- and virus-specific cytotoxicity has also been demonstrated in ginbuna and rainbow trout. Furthermore, the important role of cell-mediated immunity rather than humoral immunity has been reported in the protection against intracellular bacterial infection. Recently, the direct antibacterial activity of CD8α+, CD4+ T-cells and sIgM+ cells in fish has been reported. In this review, we summarize the recent progress in T cell research focusing on the tissue distribution and function of fish T cells.

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Role of CD4+ and CD8α+ T cells in protective immunity against Edwardsiella tarda infection of ginbuna crucian carp, Carassius auratus langsdorfii

Cited by 50 publications

References 51 publications

First Demonstration of Antigen Induced Cytokine Expression by CD4-1+ Lymphocytes in a Poikilotherm: Studies in Zebrafish (Danio rerio)

First Demonstration of Antigen Induced Cytokine Expression by CD4-1+ Lymphocytes in a Poikilotherm: Studies in Zebrafish (Danio rerio)

A DNA Vaccine Encoding the VAA Gene of Vibrio anguillarum Induces a Protective Immune Response in Flounder

T Cells in Fish

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