Role of caveolin-3 in lymphocyte activation

Abstract

Aims Caveolins are structural proteins clustered in lipid-rich regions of plasma membrane involved in coordinating signal transduction in various organ systems. While caveolin-1 (Cav-1) has been shown to regulate lymphocyte activation, the role of caveolin-3 (Cav-3) in immune system signaling has not been investigated. We tested the hypothesis that Cav-3 modulates lymphocyte activation. Main methods Lymphocyte/leukocyte subpopulations from WT and Cav-3 mice were profiled with flow cytometry. Cytokine production in quiescent and activated splenocytes from WT and Cav-3 mice was assessed with ELISA. Key findings Levels of T-cells, monocytes, and natural killer cells were not different between WT and KO mice, however KO mice had lower B-cell population-percentage. Functionally, activated lymphocytes from Cav-3 KO mice demonstrated significantly reduced expression of IL-2 compared to WT, while expression of TNFα, IL-6, and IL-10 was not different. Finally, expression of IL-17 was significantly reduced in T-helper cells from KO mice, while IFNγ was not, suggesting that Cav-3 is a determinant in the development of the Th-17 subpopulation. Significance This study is the first to demonstrate that Cav-3 may be a novel participant in B-cell expression, T-cell cytokine production and activation of inflammation.

Osteoimmune Properties of Mesoporous Bioactive Nanospheres: A Study on T Helper Lymphocytes

“…Genistein inhibits multiple tyrosine kinases of the Src family, which affects various processes such as endocytosis or chemotactic signaling, and, therefore, the dynamics of caveolae [30]. Recently, the importance of caveolin 3 expression in lymphocyte activation and differentiation was demonstrated [31]. In addition, genistein is directly involved in actin polymerization in CD4 + Th2 memory cells [32], and this alteration could favor the uptake of nanoMBGs by SR.D10 lymphocytes.…”