Role of calcium and phosphoinositide cell regulatory system in adaptation of neurons in olfactory cortex section toIn Vitro hypoxia

Tyulkova, E. I.; Semenov, D. G.; Самойлов, М. О.

doi:10.1007/bf02496865

Cited by 2 publications

(3 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The method used for studying the dynamics of Ca-c content in brain tissues was based on microfluorimetry using a chlortetracycline fluorescence probe and a LYuMAM-KF contact microscope; this method has been described in detail elsewhere, for in vivo experiments [5,8], and was modified for in vitro conditions [10]. Microregions of slices (150 Ixm in diameter) were used for measurement of the fluorescence intensity of calcium-chlortetracycline complexes bound to intracellular hydrophobic domains, using an excitation wavelength of 407 nm and an emission wavelength of 522 nm.…”

Section: Methodsmentioning

confidence: 99%

“…We have recently demonstrated that anoxia of different durations and anoxic conditioning have similar effects on the activities of these systems in cortex slices from rats [10]. The same system was used to show that anoxia lasting 2 min induces the synthesis and release of neuromediator peptides into the extracellular medium of slices; transfer of the medium to recipient slices increased the resistance of neurons in the recipient slices to anoxia lasting 10 rain, preventing depression of synaptic transmission [3,4].…”

mentioning

confidence: 99%

See 1 more Smart Citation

Involvement of intracellular regulatory systems in the adaptive effects of transient anoxia in vitro

Semenov

Tyul'kova

Самойлов

et al. 2000

Neurosci Behav Physiol

Self Cite

View full text Add to dashboard Cite

The involvement of the calcium and phosphoinositide intracellular regulatory systems in the molecular-cellular mechanisms of adaptation of the brain to hypoxia induced by transient anoxia were studied in slices of rat olfactory cortex. Anoxia lasting 2 min initiated the development of moderate but stable activation of intracellular regulatory systems during the reoxygenation period, with increases in binding of Ca2+ to intracellular hydrophobic domains and increases in the level of polyphosphoinositide metabolism. During this period, cells in the slices released neuromediator factors into the perfusion fluid; transfer of these to recipient slices induced similar changes in the activities of intracellular regulatory system components in the recipient slices. After anoxia lasting 10 min, NMDA-mediated pathogenic hyperactivity of the calcium and phosphoinositide systems developed. Preliminary moderate activation of these systems by transient anoxia or neuromodulator factors released by cells in response to transient anoxia prevented disruption of intracellular regulatory system activity induced by subsequent longer-lasting anoxia.

show abstract

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Involvement of intracellular regulatory systems in the adaptive effects of transient anoxia in vitro

Semenov

Tyul'kova

Самойлов

et al. 2000

Neurosci Behav Physiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Still, the former phenomenon might play a signalling role for the induction of long-lasting tolerance. There is evidence that the mechanism of rapid preconditioning is associated with increased excitability of neurons [10][11][12], activation of A1 adenosine receptors [13], moderate increase in intracellular free and bound Ca 2+ levels and activation of phosphoinositide hydrolysis [14,15], and activation of protein kinase C [16,17], to mention only some examples of signal-related rapid responses to short-term anoxia. Nevertheless, the data accumulated so far are incomplete and no postulation of any unitary mechanism of rapid anoxic preconditioning can yet be made.…”

Section: Introductionmentioning

confidence: 99%

Calcium Transients in the Model of Rapidly Induced Anoxic Tolerance in Rat Cortical Slices: Involvement of NMDA Receptors

2002

View full text Add to dashboard Cite

In this study, we investigated the effects of NMDA receptor antagonists on calcium transients induced by a single 2-min preconditioning anoxia (PA) in rat olfactory cortical slices, and on the ability of PA to prevent pathological calcium overload induced by subsequent 10-min test anoxia (TA). Relative changes in the intracellular Ca²⁺ concentration (Ca_i) and in the level of Ca²⁺ bound to intracellular hydrophobic domains (Ca_b) were monitored using fura-2 and chlortetracycline, respectively. Our results confirmed that TA induces prominent long-lasting increases in Ca_i and Ca_b, reflecting cellular calcium overload. It was found that PA produces moderate increases in both Ca²⁺ pools and prevents Ca²⁺ overload induced by TA carried out 90 min later. Calcium transients and the protective effects of PA were significantly suppressed in slices treated with NMDA receptor antagonists during and 30 min after PA. These results indicate that moderate activation of the NMDA receptors participates in the mechanism of the PA-induced anoxic tolerance of cortical neurons.

show abstract

Role of calcium and phosphoinositide cell regulatory system in adaptation of neurons in olfactory cortex section toIn Vitro hypoxia

Cited by 2 publications

References 5 publications

Involvement of intracellular regulatory systems in the adaptive effects of transient anoxia in vitro

Involvement of intracellular regulatory systems in the adaptive effects of transient anoxia in vitro

Calcium Transients in the Model of Rapidly Induced Anoxic Tolerance in Rat Cortical Slices: Involvement of NMDA Receptors

Contact Info

Product

Resources

About