Role of Ca2+-activated K+ channels and Na+,K+-ATPase in prostaglandin E1- and E2-induced inhibition of the adrenergic response in human vas deferens

Medina, Pascual; Segarra, Gloria; Mauricio, Maria D.; Vila, José M.; Chuan, Pascual; Lluch, Salvador

doi:10.1016/j.bcp.2011.03.021

Cited by 7 publications

(5 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…58 In contrast, a large body of evidence demonstrates that PGE 1 and PGE 2 inhibit the release of norepinephrine from sympathetic nerve endings and also suppresses the constrictor response of the effector cells to nerve stimulation, and may serve as a break against sympathoactivation in the peripheral tissues. 61,62 Therefore, the E series of PGs can exert either positive or negative influence on sympathoactivation depending on the type of tissues.…”

Section: And Sympathoactivationmentioning

confidence: 99%

Distinct Roles of Central and Peripheral Prostaglandin E2 and EP Subtypes in Blood Pressure Regulation

Yang

2012

Am J Hypertens

View full text Add to dashboard Cite

Prostaglandin E(2) (PGE(2)) is a major prostanoid with a wide variety of biological activities. PGE(2) can influence blood pressure (BP) both positively and negatively. In particular, centrally administered PGE(2) induces hypertension whereas systemic administration of PGE(2) produces a hypotensive effect. These physiologically opposing effects are generated by the existence of multiple EP receptors, namely EP(1-4), which are G protein-coupled receptors with distinct signaling properties. This review highlights the distinct roles of PGE(2) in BP regulation and the involvement of specific EP receptor subtypes.

show abstract

Section: And Sympathoactivationmentioning

confidence: 99%

Distinct Roles of Central and Peripheral Prostaglandin E2 and EP Subtypes in Blood Pressure Regulation

Yang

2012

Am J Hypertens

View full text Add to dashboard Cite

show abstract

“…On a comparative, applied basis, we propose the rat vas deferens might be a relevant experimental model with which to study novel drugs of clinical importance in the treatment of human erectile dysfunction [27,28]. Moreover, the present observations might have a clinical correlate linked to the human sexual response because blockade of vas deferens motility through inhibition of P2X1R activity might be considered a putative mechanism for an alleged male contraceptive opportunity.…”

Section: Donoso Et Almentioning

confidence: 83%

P2X1 Receptors Localized in Lipid Rafts Mediate ATP Motor Responses in the Human Vas Deferens Longitudinal Muscles1

Donoso

Norambuena

Navarrete

et al. 2014

Biology of Reproduction

View full text Add to dashboard Cite

To assess the role of the P2X1 receptors (P2X1R) in the longitudinal and circular layers of the human vas deferens, ex vivo-isolated strips or rings were prepared from tissue biopsies to record isometric contractions. To ascertain its membrane distribution, tissue extracts were analyzed by immunoblotting following sucrose gradient ultracentrifugation. ATP, alpha,beta-methylene ATP, or electrical field stimulation elicited robust contractions of the longitudinal layer but not of the circular layer which demonstrated inconsistent responses. Alpha,beta-methylene ATP generated stronger and more robust contractions than ATP. In parallel, prostatic segments of the rat vas deferens were examined. The motor responses in both species were not sustained but decayed within the first minute, showing desensitization to additional applications. Cross-desensitization was established between alpha,beta-methylene ATP or ATP-evoked contractions and electrical field stimulation-induced contractions. Full recovery of the desensitized motor responses required more than 30 min and showed a similar pattern in human and rat tissues. Immunoblot analysis of the human vas deferens extracts revealed a P2X1R oligomer of approximately 200 kDa under nonreducing conditions, whereas dithiothreitol-treated extracts showed a single band of approximately 70 kDa. The P2X1R was identified in ultracentrifugation fractions containing 15%-29% sucrose; the receptor localized in the same fractions as flotillin-1, indicating that it regionalized into smooth muscle lipid rafts. In conclusion, ATP plays a key role in human vas deferens contractile responses of the longitudinal smooth muscle layer, an effect mediated through P2X1Rs.

show abstract

“…Although PGs are not sufficiently synthesized under basal conditions, these lipids may affect contractility of human vas deferens. In isolated human vas deferens, both PGE 1 and PGE 2 inhibited adrenergic responses by a prejunctional mechanism that involves the activation of large-conductance Ca 2+ -activated K + channels and Na + /K + -ATPase (Medina et al, 2011). On the other hand, it was reported that PGE 2 itself induces contractile response in the rat vas deferens (Amadi et al, 1999).…”

Section: Ep1-4 and Ip Receptors In Human And Rodentsmentioning

confidence: 95%

International Union of Basic and Clinical Pharmacology. CIX. Differences and Similarities between Human and Rodent Prostaglandin E₂Receptors (EP1–4) and Prostacyclin Receptor (IP): Specific Roles in Pathophysiologic Conditions

et al. 2020

View full text Add to dashboard Cite

Role of Ca2+-activated K+ channels and Na+,K+-ATPase in prostaglandin E1- and E2-induced inhibition of the adrenergic response in human vas deferens

Cited by 7 publications

References 35 publications

Distinct Roles of Central and Peripheral Prostaglandin E2 and EP Subtypes in Blood Pressure Regulation

Distinct Roles of Central and Peripheral Prostaglandin E2 and EP Subtypes in Blood Pressure Regulation

P2X1 Receptors Localized in Lipid Rafts Mediate ATP Motor Responses in the Human Vas Deferens Longitudinal Muscles1

International Union of Basic and Clinical Pharmacology. CIX. Differences and Similarities between Human and Rodent Prostaglandin E₂Receptors (EP1–4) and Prostacyclin Receptor (IP): Specific Roles in Pathophysiologic Conditions

Contact Info

Product

Resources

About

Role of Ca2+-activated K+ channels and Na+,K+-ATPase in prostaglandin E1- and E2-induced inhibition of the adrenergic response in human vas deferens

Cited by 7 publications

References 35 publications

Distinct Roles of Central and Peripheral Prostaglandin E2 and EP Subtypes in Blood Pressure Regulation

Distinct Roles of Central and Peripheral Prostaglandin E2 and EP Subtypes in Blood Pressure Regulation

P2X1 Receptors Localized in Lipid Rafts Mediate ATP Motor Responses in the Human Vas Deferens Longitudinal Muscles1

International Union of Basic and Clinical Pharmacology. CIX. Differences and Similarities between Human and Rodent Prostaglandin E2Receptors (EP1–4) and Prostacyclin Receptor (IP): Specific Roles in Pathophysiologic Conditions

Contact Info

Product

Resources

About

International Union of Basic and Clinical Pharmacology. CIX. Differences and Similarities between Human and Rodent Prostaglandin E₂Receptors (EP1–4) and Prostacyclin Receptor (IP): Specific Roles in Pathophysiologic Conditions