Role of CA125 in predicting ovarian cancer survival - a review of the epidemiological literature

Gupta, Digant; Lis, Christopher G.

doi:10.1186/1757-2215-2-13

Cited by 170 publications

(113 citation statements)

References 80 publications

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“…Therefore, we determined 35-65 U/ml as the normal range, thereby increasing the test's sensitivity. We used more than 60 U/ml as the risk level [46]. The CA-125 level was increased in ovarian cancer, but it may also be elevated in benign diseases including myoma uteri, adenomyosis, pelvic inflammation and endometriosis, pregnancy, and endometrioma (benign ovarian tumor) or other ovarian cysts.…”

Section: Discussionmentioning

confidence: 99%

Effect of the Multiple Intravenous Administration of Cultured Human Autologous Adipose-Derived Stem Cells on Tumor Biomarker Levels

Chang-hyun¹,

Kim²,

Kim³

2017

J Clin Case Rep

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Many studies have been conducted to cultivate and utilize patients' own adipose-derived stem cells for the treatment of various incurable diseases and for regenerative medicine that can prolong lifespan. Despite the significant achievements made thus far, the lack of confidence with regard to safety, particularly the concern about tumorigenicity, has made researchers hesitant to actively apply cultured human autologous adipose-derived cells in clinical trials. Therefore, studies on the tumorigenicity of cultured adipose-derived stem cells are very important for expanding the field of stem cell-utilizing regenerative medicine. It is also important to study their effect on tumor biomarker levels. Long-term follow-up studies of tumorigenicity after multiple intravenous administrations of cultured human autologous adipose-derived stem cells have not been reported worldwide. Therefore, the authors have examined 462 Koreans who were administered more than 1 billion cultured autologous adipose-derived stem cells multiple times from 2010 to 2013 at medical institutions in Japan. We then conducted the first retrospective research in the world on the changes in eight types of tumor biomarkers over three-six years. According to the results of our analysis, there were no significant changes in the observed tumor biomarkers, irrespective of gender and age. These results suggest that multiple administrations of autologous adipose-derived stem cells cultured in accordance with the authors' method do not affect tumorigenicity.

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Section: Discussionmentioning

confidence: 99%

Effect of the Multiple Intravenous Administration of Cultured Human Autologous Adipose-Derived Stem Cells on Tumor Biomarker Levels

Chang-hyun¹,

Kim²,

Kim³

2017

J Clin Case Rep

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“…Elevated levels of this antigen are detected in over 90% of sera of disseminated ovarian cancer cases but only in 50% of patients in the early stages of the disease [10]. CA 125 screening is used with the addition of ultrasound screening.…”

Section: Introductionmentioning

confidence: 99%

“…The most commonly used biomarker for ovarian cancer is Cancer Antigen 125 (CA125) or Mucin16 which is thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces [9]. Elevated levels of this antigen are detected in over 90% of sera of disseminated ovarian cancer cases but only in 50% of patients in the early stages of the disease [10]. CA 125 screening is used with the addition of ultrasound screening.…”

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confidence: 99%

Epitope presentation is an important determinant of the utility of antigens identified from protein arrays in the development of autoantibody diagnostic assays

Murphy

O’Connell

O’Kane

et al. 2012

Journal of Proteomics

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* These authors contributed equally.Autoantibodies represent an attractive biomarker for diagnostic assays principally due to the stability of immunoglobulin in patient serum facilitating measurement with conventional assays. Immune responses to tumorigenesis may facilitate detection of ovarian cancer in the early stages of the disease with identification of a panel of tumour specific autoantibodies. Despite the reporting of many tumour associated autoantibodies using arrays of tumour antigens, this has not led to the advance in diagnostic capability as rapidly as was initially expected. Here we examine the potential diagnostic utility of candidate autoantibody biomarkers identified via screening of serum samples on a high content human protein array from a unique cohort of early stage and late stage ovarian cancer patients. We analyse the performance of autoantibodies to the tumour suppressor protein p53 and the novel autoantigens alpha adducin and endosulfine alpha identified in our array screen. Each antigen has different performance characteristics using conventional ELISA format and Western blot immunoassay. The high attrition rate of promising autoantigens identified by array screening can in part be explained by the presentation of the epitope of the antigen in the subsequent method of validation and this study provides directions on maximising the potential of candidate biomarkers. The most commonly used biomarker for ovarian cancer is Cancer Antigen 125 (CA125) or Mucin16 which is thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces [9]. Elevated levels of this antigen are detected in over 90% of sera of disseminated ovarian cancer cases but only in 50% of patients in the early stages of the disease [10]. CA 125 screening is used with the addition of ultrasound screening. Although these combined approaches A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT Materials and Methods Serum Sample DetailsSerum samples and clinical information was obtained with informed consent and study approval was obtained from St. James's Hospital and Adelaide and Meath incorporating the National Childerns Hospital research ethics committee.Pre-operative bloods were obtained from patients undergoing cytoreductive surgery for possible ovarian neoplasm. Blood was collected into a non-heparinised tube and allowed to clot, then centrifuged at 400 x g for 15 min. The serum supernatant was removed and dispensed into labelled cryovial tubes. Serum was stored at -80 o C until further use. hEx1 Serum Screening of Ovarian Cancer SamplesThe hEx1 array PVDF membranes (each hEx1 array is made up of 2 PVDF membranes, pt 8 and pt 9) were activated in ethanol for 1 min, rinsed in deionised water and washed in tris-buffered saline-T-T (500mM NaCl, 10mM Tris-HCl pH 7.5, 0.05% v/v Tween, 0.5% v/v Triton X ). Dessicated bacterial colonies were removed with tissue. The arrays washed in TBS-T-T for 10 min, TBS-T (500mM NaCl, 10mMTris-HCl pH 7.5, 0.05% v/v Tween) for 10 min and TB...

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“…Although certain ovarian cancer screening tests have been shown to decrease mortality rates, the possibility of efficient screening that may be used in everyday practice remains elusive. Measurement of serum CA125 antigen remains the gold standard (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18).…”

Section: Introductionmentioning

confidence: 99%

HE4 protein and SMRP: Potential novel biomarkers in ovarian cancer detection

et al. 2012

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Role of CA125 in predicting ovarian cancer survival - a review of the epidemiological literature

Cited by 170 publications

References 80 publications

Effect of the Multiple Intravenous Administration of Cultured Human Autologous Adipose-Derived Stem Cells on Tumor Biomarker Levels

Effect of the Multiple Intravenous Administration of Cultured Human Autologous Adipose-Derived Stem Cells on Tumor Biomarker Levels

Epitope presentation is an important determinant of the utility of antigens identified from protein arrays in the development of autoantibody diagnostic assays

HE4 protein and SMRP: Potential novel biomarkers in ovarian cancer detection

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