Role of BP*C@AgNPs in Bap-dependent multicellular behavior of clinically important methicillin-resistant Staphylococcus aureus (MRSA) biofilm adherence: A key virulence study

Xu, Zeling; Manukumar, H.M.; Rakesh, K.P.; Karthik, C.S.; Prasad, H.S. Nagendra; Swamy, Sachidananda; Mallu, P.; Mohammed, Yasser Hussein Eissa; Qin, Hua‐Li

doi:10.1016/j.micpath.2018.07.025

Cited by 50 publications

(14 citation statements)

References 41 publications

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“…HTCs-capsaicin exhibits high antibacterial efficacy against all bacterial strains. The antibacterial efficiency of honey, chitosan, AuNPs, and capsaicin have been confirmed in Gram-positive and-negative bacteria [ 51 ]. Moreover, the increased antibacterial efficiency against V. vulnificus compared with S. pyogenes has been emphasized in previous studies for chitosan [ 52 ] as well as capsaicin extract and a mixture of honey and AuNPs [ 53 ].…”

Section: Resultsmentioning

confidence: 99%

Antibacterial Activity of Honey/Chitosan Nanofibers Loaded with Capsaicin and Gold Nanoparticles for Wound Dressing

Al-Musawi¹,

Albukhaty

Al-Karagoly

et al. 2020

Molecules

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This paper describes the preparation, characterization, and evaluation of honey/tripolyphosphate (TPP)/chitosan (HTCs) nanofibers loaded with capsaicin derived from the natural extract of hot pepper (Capsicum annuumL.) and loaded with gold nanoparticles (AuNPs) as biocompatible antimicrobial nanofibrous wound bandages in topical skin treatments. The capsaicin and AuNPs were packed within HTCs in HTCs-capsaicin, HTCs-AuNP, and HTCs-AuNPs/capsaicin nanofibrous mats. In vitro antibacterial testing against Pasteurella multocida, Klebsiella rhinoscleromatis,Staphylococcus pyogenes, and Vibrio vulnificus was conducted in comparison with difloxacin and chloramphenicol antibiotics. Cell viability and proliferation of the developed nanofibers were evaluated using an MTT assay. Finally, in vivo study of the wound-closure process was performed on New Zealand white rabbits. The results indicate that HTCs-capsaicin and HTCs-AuNPs are suitable in inhibiting bacterial growth compared with HTCs and HTCs-capsaicin/AuNP nanofibers and antibiotics (P < 0.01). The MTT assay demonstrates that the nanofibrous mats increased cell proliferation compared with the untreated control (P < 0.01). In vivo results show that the developed mats enhanced the wound-closure rate more effectively than the control samples. The novel nanofibrous wound dressings provide a relatively rapid and efficacious wound-healing ability, making the obtained nanofibers promising candidates for the development of improved bandage materials.

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Section: Resultsmentioning

confidence: 99%

Antibacterial Activity of Honey/Chitosan Nanofibers Loaded with Capsaicin and Gold Nanoparticles for Wound Dressing

Al-Musawi¹,

Albukhaty

Al-Karagoly

et al. 2020

Molecules

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“…In the study, using well diffusion test by loading different concentrations of synthetic BP*C@AgNPs against biofilm producing MRSA, depicted the dose-dependent membrane damage leading to bacterial killing. The study also depicted the role of BP*C@AgNPs in the inhibition of biofilm synthesis leading to the decreased adherence of bacterial cells to each other 87…”

Section: Introductionmentioning

confidence: 92%

“…However, some vaccine candidates failed to show significant protection and this may be because of overreliance on the antibody-mediated protective response 88Table 1Candidate vaccines for active immunizationTarget antigenStudy phaseOutcomeReferencesCP5 and CP8Phase IIINo significant protection against bacteremia in patients receiving hemodialysis when compared with placebo87CP5-CRM 197 , CP8-CRM 197 , and ClfA (SA3Ag)Phase IShowed significant neutralizing antibody response91,93CP5-CRM 197 , CP8-CRM 197 , MntC, and ClfA (SA4Ag)Phase IShowed robust immune response, safe, and well-tolerated and phase 2b is ongoing91,97Alpha-toxin and Panton-Valentine LeukocidinPhase IShowed a good toxin neutralizing sero-positive response103EsxA and EsxBPreclinicalShowed protection with improving survival of murine model106Surface protein A (SpA)PreclinicalShowed protection in mouse model110,111D-alanine auxotrophic Staphylococcus aureus PreclinicalShowed protection from the formation of abscesses and improved survival in immunized mice113AdsAPreclinicalShowed protection in the immunized mouse model114Coa (Hc-CoaR6)PreclinicalShowed a strong T-cell response and protection in mice against lethal dose of S. aureus118Staphylococcal enterotoxin BPreclinicalShowed an efficient protection in BALB/c mice122…”

Section: Recent Developments In Active Immunizationmentioning

confidence: 99%

<p>Recent advances in <em>Staphylococcus aureus</em> infection: focus on vaccine development</p>

Ansari

Jha

Mishra

et al. 2019

IDR

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Staphylococcus aureus normally colonizes the nasal cavity and pharynx. After breaching the normal habitat, the organism is able to cause a number of infections at any site of the body. The development of antibiotic resistance has created a global challenge for treating infections. Therefore, protection by vaccines may provide valuable measures. Currently, several vaccine candidates have been prepared which are either in preclinical phase or in early clinical phase, whereas several candidates have failed to show a protective efficacy in human subjects. Approaches have also been made in the development of monoclonal or polyclonal antibodies for passive immunization to protect from S. aureus infections. Therefore, in this review we have summarized the findings of recently published scientific literature to make a concise report.

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“…Biofilm, a surface‐associated bacterial community surrounded by a self‐produced extracellular matrix 21–25 associated with resistance to antimicrobial agents 26,27 . Recently, new materials such as dihydrazone analogues against biofilm forming Gram-negative 28 and benzodioxane midst piperazine decorated chitosan silver nanoparticles (BP*C@AgNPs) was controlled the MRSA biofilm 29 , still indole and its derivatives have been regarded as interesting heterocyclic antibacterial molecules against AMR bacteria owing to their promising potential to inhibit the formation of biofilm. In addition, the modification of indoles with diverse functional groups would change the efficiency of biofilm inhibition and the antibacterial specificity.…”

Section: Introductionmentioning

confidence: 99%

Design, synthesis, and discovery of novel oxindoles bearing 3-heterocycles as species-specific and combinatorial agents in eradicating Staphylococcus species

Shin

Magar

Lee

et al. 2019

Sci Rep

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A series of new functionalized 3-indolylindolin-2-ones, 3-(1-methylpyrrol-2-yl)indolin-2-ones, and 3-(thiophen-2-yl)indolin-2-ones were synthesized by using novel indium (III)-catalysed reaction of various 3-diazoindolin-2-ones with indoles, 1-methylpyrrole, or thiophene via one-pot procedure. The newly synthesized compounds were characterized and screened for their in vitro antibacterial activity against various Staphylococcus species, including methicillin-resistant Staphylococcus aureus. results revealed that five compounds KS15, KS16, KS17, KS19, and KS20 exhibited potent and specific antibacterial activity against Staphylococcus species albeit inactive against Gram-negative bacteria. Especially, compounds exhibited superior antibacterial potency against Staphylococcus epidermidis compared to the reference drug streptomycin. The most potential compound KS16 also increased the susceptibility of Staphylococcus aureus to ciprofloxacin, gentamicin, kanamycin, and streptomycin. Among them, KS16 was found to be a synergistic compound with gentamicin and kanamycin. Furthermore, the cellular level of autolysin protein was increased from the KS16-treated Staphylococcus aureus cells. Finally, in vitro CCK-8 assays showed that KS16 exhibited no cytotoxicity at the minimum inhibitory concentrations used for killing Staphylococcus species. From all our results, novel oxindole compounds directly have lethal action or boost existing antibiotic power with the reduction of doses and toxicity in the treatment of multidrug-resistant Staphylococcus species.

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Role of BP*C@AgNPs in Bap-dependent multicellular behavior of clinically important methicillin-resistant Staphylococcus aureus (MRSA) biofilm adherence: A key virulence study

Cited by 50 publications

References 41 publications

Antibacterial Activity of Honey/Chitosan Nanofibers Loaded with Capsaicin and Gold Nanoparticles for Wound Dressing

Antibacterial Activity of Honey/Chitosan Nanofibers Loaded with Capsaicin and Gold Nanoparticles for Wound Dressing

<p>Recent advances in <em>Staphylococcus aureus</em> infection: focus on vaccine development</p>

Design, synthesis, and discovery of novel oxindoles bearing 3-heterocycles as species-specific and combinatorial agents in eradicating Staphylococcus species

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