Role of bone morphogenetic protein‐9 in the regulation of glucose and lipid metabolism

Abstract

Bone morphogenetic protein (BMP)‐9 has been reported to regulate energy balance in vivo. However, the mechanisms underlying BMP9‐mediated regulation of energy balance remain incompletely understood. Here, we investigated the role of BMP9 in energy metabolism. In the current study, we found that hepatic BMP9 expression was down‐regulated in insulin resistance (IR) mice and in patients who are diabetic. In mice fed a high‐fat diet (HFD), the overexpression of hepatic BMP9 improved glucose tolerance and IR. The expression of gluconeogenic genes was down‐regulated, whereas the level of insulin signaling molecule phosphorylation was increased in the livers of Adenovirus‐BMP9‐treated mice and glucosamine‐treated hepatocytes. Furthermore, BMP9 overexpression ameliorated triglyceride accumulation and inhibited the expression of lipogenic genes in both human hepatocellular carcinoma HepG2 cells treated with a fatty acid mixture as well as the livers of HFD‐fed mice. In hepatocytes isolated from sterol regulatory element‐binding protein (SREBP)‐1c knockout mice, the effects of BMP9 were ablated. Mechanistically, BMP9 inhibited SREBP‐1c expression through the inhibition of liver X receptor response element 1 activity in the SREBP‐1c promoter. Taken together, our results show that BMP9 is an important regulator of hepatic glucose and lipid metabolism.—Yang, M., Liang, Z., Yang, M., Jia, Y., Yang, G., He, Y., Li, X., Gu, H. F., Zheng, H., Zhu, Z., Li, L. Role of bone morphogenetic protein‐9 in the regulation of glucose and lipid metabolism. FASEB J. 33, 10077–10088 (2019). http://www.fasebj.org

RETRACTED ARTICLE: Schnurri-3 regulates BMP9-induced osteogenic differentiation and angiogenesis of human amniotic mesenchymal stem cells through Runx2 and VEGF

“…Cells were seeded in six-well plates and treated as per the experimental design. Total protein were obtained after lysis, and cleared lysates were denatured by boiling for 10 min with 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis as described previously 78 . Page Ruler Plus Pre-stained Protein Ladder (26619; Thermo Scientific, USA) was used to evaluate the bands based on molecular weights ranging from 10 to 250 kDa.…”

Association between circulating follistatin‐like‐1 and metabolic syndrome in middle‐aged and old population: A cross‐sectional study

“…These proteins are at the core of the FSTL‐1 protein interaction network. The interactive proteins listed in this study were mainly enriched in biological pathologic processes in response to adipocyte differentiation and inflammation of CYR61, 28 adipogenesis, inflammation, and angiogenesis for SPARC1 29‐31 ; hypertension and atherosclerotic lesions for LTBP1 32,33 ; lipolysis in skeletal muscle, inflammatory processes and IR for IL6 34 ; the differentiation of preadipocytes into white adipocytes for BMP2 35 ; hepatic lipid metabolism for BMP4 36 and non‐alcoholic fatty liver disease (NAFLD) for FSTL‐3 37 . In accord with the above analysis results, our Co‐IP experiment further confirmed the interaction between FSTL‐1 and BMP4 in vivo.…”

Genetic ablation of C-reactive protein gene confers resistance to obesity and insulin resistance in rats

“…Primer sequences are listed in electronic supplementary material (ESM) Table 1. Protein expression was analysed by western blot, as indicated previously [20]. Basic information on all the antibodies used in this study is listed in ESM Table 2.…”