Role of bone marrow derived mesenchymal stromal cells and Schwann-like cells transplantation on spinal cord injury in adult male albino rats

Galhom, Rania A.; Raouf, Hoda H. Hussein Abd El; Ali, Mohammed

doi:10.1016/j.biopha.2018.09.131

Cited by 14 publications

(8 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, undifferentiated BM-MSCs better support axonal regeneration, while BM-MSCs-derived SCLCs promote significant remyelination. Therefore, the authors suggest that a combination of SCLCs and BM-MSCs may become effective in treating SCI [118]. The application of human-MSC-derived SCLCs in SCI is still lacking.…”

Section: Application In the Cnsmentioning

confidence: 99%

Schwann Cell-Like Cells: Origin and Usability for Repair and Regeneration of the Peripheral and Central Nervous System

Hopf

Schaefer

Kalbermatten

et al. 2020

Cells

View full text Add to dashboard Cite

Functional recovery after neurotmesis, a complete transection of the nerve fiber, is often poor and requires a surgical procedure. Especially for longer gaps (>3 mm), end-to-end suturing of the proximal to the distal part is not possible, thus requiring nerve graft implantation. Artificial nerve grafts, i.e., hollow fibers, hydrogels, chitosan, collagen conduits, and decellularized scaffolds hold promise provided that these structures are populated with Schwann cells (SC) that are widely accepted to promote peripheral and spinal cord regeneration. However, these cells must be collected from the healthy peripheral nerves, resulting in significant time delay for treatment and undesired morbidities for the donors. Therefore, there is a clear need to explore the viable source of cells with a regenerative potential similar to SC. For this, we analyzed the literature for the generation of Schwann cell-like cells (SCLC) from stem cells of different origins (i.e., mesenchymal stem cells, pluripotent stem cells, and genetically programmed somatic cells) and compared their biological performance to promote axonal regeneration. Thus, the present review accounts for current developments in the field of SCLC differentiation, their applications in peripheral and central nervous system injury, and provides insights for future strategies.

show abstract

Section: Application In the Cnsmentioning

confidence: 99%

Schwann Cell-Like Cells: Origin and Usability for Repair and Regeneration of the Peripheral and Central Nervous System

Hopf

Schaefer

Kalbermatten

et al. 2020

Cells

View full text Add to dashboard Cite

show abstract

“…Then 1-μm-thick semithin sections were obtained, stained with toluidine blue, examined, and photographed under a light microscope. The ultrathin sections were cut using an ultratome (Reichert Ultracut, Ziess, Germany), stained with uranyl acetate and lead citrate, and examined and photographed under a transmission electron microscope (1230 EXII; JOEL, Tokyo, Japan) (Mycotic Center, Al-Azhar university) ( Galhom et al, 2018 ).…”

Section: Methodsmentioning

confidence: 99%

Urine-Derived Stem Cells Versus Their Lysate in Ameliorating Erectile Dysfunction in a Rat Model of Type 2 Diabetes

et al. 2022

Self Cite

View full text Add to dashboard Cite

Background: Diabetic erectile dysfunction (DED) is a significant consequence of diabetes mellitus, and it is a multifactorial phenomenon that has no definitive treatment until now. Many therapeutic options provide symptomatic improvement rather than addressing the underlying etiology or restoring normal function. Stem cell (SC) therapy represents a potential hope in DED management. It is well established that the regenerative effect of stem cells can be attained by their paracrine action and their ability to differentiate into many cell lineages, including endothelial and smooth muscle cells. Hence, we tried to compare the effects of transplantation of urine-derived stem cells (USCs) or their lysate (USC-L) into the corpora cavernosa (CCs) of rats with DED.Materials and Methods: A total of 55 adult male Wistar rats were included in this study. USCs were obtained from ten healthy rats. Another ten rats did not subject to any intervention and served as a control (group I). Type 2 DM and DED were induced in the remaining 35 rats, but DED was tested and proved in only 24 rats, which were randomly divided into three groups (n = 8 in each). The DED group (group II) and either USCs (2 × 106 cells) or their lysate (200 μl) were transplanted into the CCs of each rat in the other two groups (groups III and IV), respectively.Results: Although the DED rats exhibited deterioration in all copulatory functions as compared to the control group, our histopathological, immunohistochemical, and morphometric results revealed that both USCs and USC-L have significantly restored the cavernous spaces, the ultrastructures of the endothelium that line the cavernous spaces, collagen/smooth muscle ratio, and the mean area percentage of α-SMA in the CCs as compared to DED rats. A respectable number of USCs was detected in the CCs of group III at the 4th week after transplantation, but this number significantly declined by the 8th week.Conclusion: Both USCs and USC-L can repair the structure and ultrastructure of CCs and improve the copulatory functions in the DED rat model. However, USC-L could be better used in DED to guard against the strange behavior of USCs after transplantation and their decreased survivability with time.

show abstract

“…Transgenic stem cells can not only improve the ability of cells to differentiate, but also express a large number of specific neurotrophic factors and reconstruct the spinal nerve circuit [50]. Many animal SCI model studies combined with gene therapy, such as NT-3 over-secreting BM-SCs [51] and hUCB-MSCs [52], CSF-induced BM-SCs [53], Schwann-like cells after BM-SCs being induced by sciatic nerve fragments [54], etc. They have all been proved to be better than simply transplanting cell in promoting functional recovery.…”

Section: Cell Transplantation Combined With Gene Therapymentioning

confidence: 99%

The cell repair research of spinal cord injury: a review of cell transplantation to treat spinal cord injury

Zhang

Wang

Song

2019

Journal of Neurorestoratology

View full text Add to dashboard Cite

Through retrospective analysis of the literature on the cell repair of spinal cord injury worldwide, it is found that the mechanism of cell transplantation repairing spinal cord injury is mainly to replace damaged neurons, protect host neurons, prevent apoptosis, promote axonal regeneration and synapse formation, promote myelination, and secrete trophic factors or growth factors to improve microenvironment. A variety of cells are used to repair spinal cord injury. Stem cells include multipotent stem cells, embryonic stem cells, and induced pluripotent stem cells. The multipotent stem cells are mainly various types of mesenchymal stem cells and neural stem cells. Non-stem cells include olfactory ensheathing cells and Schwann cells. Transplantation of inhibitory interneurons to alleviate neuropathic pain in patients is receiving widespread attention. Different types of cell transplantation have their own advantages and disadvantages, and multiple cell transplantation may be more helpful to the patient’s functional recovery. These cells have certain effects on the recovery of neurological function and the improvement of complications, but further exploration is needed in clinical application. The application of a variety of cell transplantation, gene technology, bioengineering and other technologies has made the prospect of cell transplantation more extensive. There is a need to find a safe and effective comprehensive treatment to maximize and restore the patient’s performance.

show abstract

Role of bone marrow derived mesenchymal stromal cells and Schwann-like cells transplantation on spinal cord injury in adult male albino rats

Cited by 14 publications

References 65 publications

Schwann Cell-Like Cells: Origin and Usability for Repair and Regeneration of the Peripheral and Central Nervous System

Schwann Cell-Like Cells: Origin and Usability for Repair and Regeneration of the Peripheral and Central Nervous System

Urine-Derived Stem Cells Versus Their Lysate in Ameliorating Erectile Dysfunction in a Rat Model of Type 2 Diabetes

The cell repair research of spinal cord injury: a review of cell transplantation to treat spinal cord injury

Contact Info

Product

Resources

About