a b s t r a c tFast urbanization has led to ozone (O 3 ) being the main pollutant in summer in most of China. To assess future ground-level O 3 effects on the service of urban greening species and clarify the underlying mechanism of O 3 damage, four common urban greening species, Ailanthus altissima (AA), Fraxinus chinensis (FC), Platanus orientalis (PO) and Robinia pseudoacacia (RP) were exposed to non-filtered air (NF) and to elevated O 3 (E-O 3 ) in open-top chambers. E-O 3 induced visible injury in all species as well as microscopic alterations such as collapse of the palisade parenchyma cells, callose accumulation, or chloroplast and mitochondrial accelerated senescence. E-O 3 significantly reduced light-saturated CO 2 assimilation (A sat ), the maximum activity of Rubisco (Vc max ), the maximum electron transport rate (J max ), and fluorescence parameters such as the quantum yield of noncyclic electron transport ( PSII ), and the quenching of photochemical efficiency of PSII (qP). It also increased total antioxidant capacity, phenolics and ascorbate contents. No significant interaction between O 3 and species was found in photosynthetic performance and antioxidant systems, suggesting that the four species selected were sensitive to O 3 . Of all four species, AA was the most sensitive species due to a combination of earlier injury onset, anatomical features, lower antioxidant responses and higher stomatal conductance. The sensitivity of tree species to O 3 is a factor to be considered for urban greening. Ozone may affect important urban forest ecosystem services by reducing CO 2 assimilation.