Role of biogenic silver nanoparticles in disruption of cell–cell adhesion in Staphylococcus aureus and Escherichia coli biofilm

Goswami, Shrestha Roy; Sahareen, T.; Singh, Mukesh; Kumar, Santosh

doi:10.1016/j.jiec.2014.11.017

Cited by 93 publications

(59 citation statements)

References 60 publications

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“…Our study revealed that MgO NPs at sub-inhibitory concentrations inhibit the biofilm-forming potential of pathogens such as E. coli, K. pneumoniae and S. aureus, which are known for their biofilm-forming capacity. We found that MgO NPs reduced the biofilm-forming capacity of bacteria by 31%-82.9% in a time-dependent manner, comparable to previous studies on silver NPs (27). However, the concentration of Ag-NPs used in that study was higher than the concentration of MgO NPs used in the current study.…”

Section: Discussionsupporting

confidence: 88%

In vitro antibiofilm and anti‐adhesion effects of magnesium oxide nanoparticles against antibiotic resistant bacteria

Hayat

Muzammil

Rasool

et al. 2018

Microbiology and Immunology

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The aim of the current investigation was to determine the antibacterial and antibiofilm potential of MgO nanoparticles (NPs) against antibiotic-resistant clinical strains of bacteria. MgO NPs were synthesized by a wet chemical method and further characterized by scanning electron microscopy and energy dispersive X-ray. Antibacterial activity was determined by broth microdilution and agar diffusion methods. The Bradford method was used to assess cellular protein leakage as a result of loss of membrane integrity. Microtiter plate assay following crystal violet staining was employed to determine the effect of MgO NPs on biofilm formation and removal of established biofilms. MIC values ranged between 125 and 500 μg/mL. Moreover, treatment with MgO NPs accelerated rate of membrane disruption, measured as a function of leakage of cellular proteins. Leakage of cellular protein content was greater among gram-negative bacteria. Cell adherence assay indicated 25.3-49.8% inhibition of bacterial attachment to plastic surfaces. According to a static biofilm method, MgO NPs reduced biofilm formation potential from 31% to 82.9% in a time-dependent manner. Moreover, NPs also significantly reduced the biomass of 48, 72, 96 and 120 hr old biofilms (P < 0.05). Cytotoxicity experiments using a neutral red assay revealed that MgO NPs are non-toxic to HeLa cells at concentrations of 15-120 μg/mL. These data provide in vitro scientific evidence that MgO NPs are effective and safe antibiofilm agents that inhibit adhesion, biofilm formation and removal of established biofilms of multidrug-resistant bacteria.

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Section: Discussionsupporting

confidence: 88%

In vitro antibiofilm and anti‐adhesion effects of magnesium oxide nanoparticles against antibiotic resistant bacteria

Hayat

Muzammil

Rasool

et al. 2018

Microbiology and Immunology

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“…They found that 50 nM of Ag-NPs significantly arrested biofilm formation without affecting viability, whereas 100 nM inhibited the growth of the P. aeruginosa itself and led to 95 % reduction in biofilm. Goswami et al (2015) also studied the Ag-NPs mediated biofilm eradication, and found inhibition of 89 % for S. aureus and 75 % for E. coli at 15 mg/ mL. The data in the present study validate that Ag-NPs can effectively and rapidly detach biofilm, produced by E. coli, P. aeruginosa, and S. aureus at clinically achievable concentrations of silver nanoparticles.…”

Section: Antibiofilm Potential Of Ag-npssupporting

confidence: 76%

Synergistic antibacterial and antibiofilm activity of silver nanoparticles biosynthesized by lignin-degrading fungus

Barapatre

Aadil

Jha

2016

Bioresour. Bioprocess.

138

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Background:The fabrication of silver nanoparticles (Ag-NPs) through green chemistry is an emerging area in the field of medical nanotechnology. Ag-NPs were fabricated by enzymatic reduction of AgNO 3 using two lignin-degrading fungus Aspergillus flavus (AfAg-NPs) and Emericella nidulans (EnAg-NPs). The prepared Ag-NPs were characterized by different spectroscopic techniques. Antibacterial activity of prepared Ag-NPs was demonstrated against selected Gram negative (Escherichia coli and Pseudomonas aeruginosa) and Gram positive (Staphylococcus aureus) bacteria in the term of minimum bactericidal concentration (MBC) and susceptibility constant (Z). The synergistic antibacterial activity of Ag-NPs with four conventional antibiotics was also determined by the fractional inhibitory concentration index (FICI) using the checkerboard microdilution method. The antibiofilm potential of Ag-NPs was also tested. Results:The plasmon surface resonance of biosynthesized Ag-NPs shows its characteristic peaks at UV and visible region (~450 and 280 nm). Fourier transform infrared spectrometer (FTIR) analysis confirms the nature of the capping agents as protein (enzyme) and indicates the role of protein (enzyme) in reduction of silver ions. The average particle size and charge of synthesized Ag-NPs was ~100 nm and ~−20 mV, respectively. X-ray diffraction (XRD) and TEM analysis confirmed the purity, shape, and size (quasi-spherical, hexagonal, and triangular) of Ag-NPs. Energy-dispersive X-ray spectroscopy (EDX) data validate the biological synthesis of Ag-NPs. Low MBC and high susceptibility constant indicate the high antimicrobial strength of biosynthesized Ag-NPs. The antibacterial analysis demonstrates the synergistic antimicrobial activity of Ag-NPs with antibiotics. This study also shows that biosynthesized Ag-NPs have ability to inhibit the biofilm formation by 80-90 %. Conclusion:The Aspergillus flavus and Emericella nidulans-mediated biosynthesized Ag-NPs have significant antimicrobial activity and demonstrate synergistic effect in combination with antibiotics. It suggests that nanoparticles can be effectively used in combination with antibiotics to improve the efficacy of antibiotics against pathogenic microbes. The substantial antibiofilm efficiency of biosynthesized Ag-NPs would also be helpful against sensitive and multidrug-resistant strains.

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“…Microorganism and different plant parts like root, leaf, seed, fruit, berry, etc. have been employed for biogenic synthesis of metal nanoparticles (Suman et al Suman et al 2013a;Jagtap and Bapat 2013;Gnanadesigan et al 2011;Kumar et al 2015;Torresdey et al 2003;Goswami et al 2015;Reddy et al 2014;Dubey et al 2010). Several studies showed that plant extract contain mostly phenolic compounds, flavonoids, terpenoids, polysaccharides, enzymes and other proteins which are responsible for reduction of metal ions and stabilization of nanoparticles (Zayed et al 2012;Suman et al 2013b;Iravani 2011).…”

Section: Introductionmentioning

confidence: 99%

Lippia javanica: a cheap natural source for the synthesis of antibacterial silver nanocolloid

et al. 2015

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Aqueous silver nanocolloid was synthesized in a single step by a biogenic approach using aqueous leaf extract of Lippia javanica plant which acts as both reducing as well as capping agent. The as-synthesized silver nanoparticles were characterized by UV-visible absorption spectroscopy, high-resolution transmission electron microscopy and Fourier transform infrared spectroscopy (FTIR). The UV-Vis absorption spectra of colloidal silver nanoparticles showed characteristic surface plasmon resonance peak centered at a wavelength of 415 nm. The kinetic study showed that the reduction process was complete within 2 h of time. The TEM analysis showed that most of the particles were spherical in shape and their average diameter was about 17.5 nm. FTIR study confirmed the presence of some organic functional groups in leaf extract and their participation during the reduction as well as stabilization process. In addition, the as-synthesized silver nanoparticles showed antimicrobial activity against clinically isolated pathogenic strain of E. coli and B. subtilis.

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Role of biogenic silver nanoparticles in disruption of cell–cell adhesion in Staphylococcus aureus and Escherichia coli biofilm

Cited by 93 publications

References 60 publications

In vitro antibiofilm and anti‐adhesion effects of magnesium oxide nanoparticles against antibiotic resistant bacteria

In vitro antibiofilm and anti‐adhesion effects of magnesium oxide nanoparticles against antibiotic resistant bacteria

Synergistic antibacterial and antibiofilm activity of silver nanoparticles biosynthesized by lignin-degrading fungus

Lippia javanica: a cheap natural source for the synthesis of antibacterial silver nanocolloid

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