Role of biocontrol agents on chlorophyll content in wilt affected lentil crop

Abstract

In the present study biocontrol agents Bacillus safensis and Bacillus licheniformis has been isolated from wilt affected lentil crop of agro-climatic zone of eastern UP like Faizabad, Mau, Varanasi, Gazipur.Lentil seed treatment has been given by these isolated strains as bioagents against wilt pathogen in pot experiment in lentil crop Rabi-season 2019-2020.Result of conducted pot experiment showed the maximum chlorophyll content was found in T3 (Bacillus safensis +Bacillus licheniformis +Fusarium oxyporum f.sp.lentis+Lentil Rasistant variety IPL-316 + Susceptible variety K-75.) followed by treatments T2 (Bacillus licheniformis +Resistant Variety IPL-316+Susceptible variety K-75 ) and T1 (Bacillus safensis +Lentil Resistant variety IPL-316 + Susceptible Variety K-75) as compare to negative control T5 (Lentil Resistant variety IPL-316 + Susceptible Variety K-75+No bacterium inoculum) and pathogen T4(Fusarium oxysporum f.spp.lentis + Resistant variety IPL-316 + Susceptible Variety K-75. The aim of this paper is to study the bio-agent's factor affecting the chlorophyll content and provide protection against pathogen in lentil plant. After that chlorophyll estimation test was performed on treated lentil crop on 30, 60 and 90 days. We observed chlorophyll content in treated crops and maximum chlorophyll content was found at 30 and 60 days which minimize at 90 days.