Role of biochemical tests in the diagnosis of exudative pleural effusions

Ernam, Dilek; Atalay, Figen; Hasanoğlu, H. Canan; Kaplan, Özgül

doi:10.1016/j.clinbiochem.2004.09.023

Cited by 19 publications

(17 citation statements)

References 15 publications

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“…There is a general agreement on the association of low glucose and high lactate dehydrogenase (LDH) pleural levels with poor prognosis in MPE (Ernam et al, 2005). For example, Sanchez-Annengol and Rodriguez-Panadero (1993), found that pleural glucose level less than 60 mg/dl is a sign for a significant shorter survival in cancer patients than in the group with higher glucose.…”

Section: Introductionmentioning

confidence: 98%

Association of High LDH and Low Glucose Levels in Pleural Space with HER2 Expression in Non-Small Cell Lung Cancer

Ziaian

Saberi²,

Ghayyoumi³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Background: Evidence shows direct link of HER2 to increased glycolysis and over production of lactate dehydrogenase (LDH). HER2 overexpression, high LDH and low glucose pleural levels are associated with poor prognosis in lung cancer. Here, their relationships were investigated. Materials and Methods: HER2 positivity was studied using immunohistochemistry in non-small cell lung cancer. Glucose and LDH levels were measured using commercial colorimetric kits. Results: Of 42 patients (29 adenocarcinoma and 13 squamous cell carcinoma), 28 (66.7%) were HER2-negative, 14 (33.3%) were HER2-positive, including 9 (21.4%) weakly stained (1+) and 5 (11.9%) moderately stained (2+) samples. The relationship between HER2 and glucose and LDH levels were tested in 20 newly diagnosed lung cancer patients who had simultaneous pleural and serum samples. Pleural and serum LDH levels were increased, and pleural glucose levels were decreased with the scale of HER2 positivity, and that the difference in glucose levels between HER2-negative group and HER2-positive patients scored at 2+ reached statistical significance (p=0.02). This latter group all had pleural glucose levels below 40 mg/dl. Conclusions: For the first time, we showed a significant association between low pleural glucose level and overexpression of HER2 in lung cancer. Further investigations are warranted to disclose the association of HER2 with low pleural glucose levels in other populations, with a larger sample size, in malignant pleural effusions caused by other types of cancer, and finally to assess employment as a screening tool for finding HER2-positive cases of lung cancer.

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Section: Introductionmentioning

confidence: 98%

Association of High LDH and Low Glucose Levels in Pleural Space with HER2 Expression in Non-Small Cell Lung Cancer

Ziaian

Saberi²,

Ghayyoumi³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…The levels of adenosine deaminase (ADA), an enzyme associated with T-lymphocyte activity, and those of interferon-gamma (IFN-c), a cytokine secreted by activated T-cells, have been shown to be increased in, and used to diagnose, tuberculous pleural effusion [5][6][7][8][9][10][11][12][13][14]. However, these increases can also be observed in other infectious, inflammatory or malignant diseases [8,[11][12][13][14][15][16][17][18][19], and are not specific for tuberculous pleurisy.…”

Section: Introductionmentioning

confidence: 99%

Enzyme-linked immunospot assay for interferon-gamma in the diagnosis of tuberculous pleurisy

Lee

Chou

Wang

et al. 2009

Clinical Microbiology and Infection

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Patients presenting with pleural effusion of undetermined aetiology were prospectively enrolled, and an enzyme-linked immunospot (ELISPOT) assay on pleural fluid and peripheral blood was performed. Forty patients were studied, including 19 with culture- or biopsy-confirmed (n = 15) or clinically compatible (n = 4) tuberculous pleurisy, and 21 with pleural effusions due to non-tuberculous causes. The sensitivity, specificity and positive and negative predictive values of the assay were 94.7%, 85.7%, 85.7% and 94.7%, respectively, on pleural fluid, and 77.8%, 90.5%, 87.5% and 82.6%, respectively, on blood. Antigen-specific, interferon-gamma-secreting T-cells were concentrated eight to ten times in pleural fluid as compared with blood. Among the seven patients not suitable for pleural biopsy and three patients whose biopsy results were non-diagnostic, nine had positive ELISPOT result with pleural fluid. The ELISPOT assay for interferon-gamma can accurately diagnose tuberculous pleurisy and is helpful for patients not suitable for pleural biopsy and those whose biopsy results are non-diagnostic.

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“…The importance of serum LDH measurement has been underscored in different conditions including malignancies (2,3 ), neuroblastoma (4 ), cutaneous melanoma (5 ), hemolytic anemia (6 ), severe acute respiratory syndrome (7 ), and chronic lymphocytic leukemia (8 ). The potential medical utility of measuring LDH in other biological fluids has also been reported (9 ). Such a pattern of utilization across various clinical settings provides a rationale for harmonization of LDH measurement results in practice.…”

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confidence: 99%

Commutable Calibrator with Value Assigned by the IFCC Reference Procedure to Harmonize Serum Lactate Dehydrogenase Activity Results Measured by 2 Different Methods

et al. 2008

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Role of biochemical tests in the diagnosis of exudative pleural effusions

Cited by 19 publications

References 15 publications

Association of High LDH and Low Glucose Levels in Pleural Space with HER2 Expression in Non-Small Cell Lung Cancer

Association of High LDH and Low Glucose Levels in Pleural Space with HER2 Expression in Non-Small Cell Lung Cancer

Enzyme-linked immunospot assay for interferon-gamma in the diagnosis of tuberculous pleurisy

Commutable Calibrator with Value Assigned by the IFCC Reference Procedure to Harmonize Serum Lactate Dehydrogenase Activity Results Measured by 2 Different Methods

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