Role of background observed in aptasensor with chemiluminescence detection

Park, Lucienne; Kim, Julie; Lee, Jihoon

doi:10.1016/j.talanta.2013.07.072

Cited by 21 publications

(14 citation statements)

References 24 publications

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“…In 2014, Duan's group reported a nano-graphite-aptamer hybrid with the action of DNase I for the amplified monitoring of OTA (Wei et al, 2015 ). Meanwhile, a one-step chemiluminescent aptasensor was developed by Lee et al, using chemically initiated electron exchange luminescence (CIEEL) between a 1,1′-oxalyldiimidazole chemiluminescence (ODI-CL) reaction and the OTA aptamer conjugated with TEX615 (Park et al, 2013 ).…”

Section: G-quadruplex-based Detection Methods For Otamentioning

confidence: 99%

Recent Progress and Development of G-Quadruplex-Based Luminescent Assays for Ochratoxin A Detection

Nao

Wang

et al. 2020

Front. Chem.

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Ochratoxin A (OTA) is a mycotoxin that is widespread throughout the world. It contaminates foods such as vegetables, fruits, and rice. It harms human health and has potential carcinogenic effects. The G-quadruplex (G4) is a tetraplexed DNA structure generated from guanine-rich DNA that has found emerging use in aptamer-based sensing systems. This review outlines the status of OTA contamination and conventional detection methods for OTA. Various G4-based methods to detect OTA developed in recent years are summarized along with their advantages and disadvantages compared to existing approaches.

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Section: G-quadruplex-based Detection Methods For Otamentioning

confidence: 99%

Recent Progress and Development of G-Quadruplex-Based Luminescent Assays for Ochratoxin A Detection

Nao

Wang

et al. 2020

Front. Chem.

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“…The rate of hybridization between MM 1, which is a double base mismatched ssDNA, and complementary probe-conjugated TEX615 was the slowest. 13), thrombin 19 ) in PBS buffer was faster than that in Tris-HCl buffer. The hybridization of MM 3 (5 0 -GTG GTG GAC TGC ATC-3 0 ) was faster than that of MM 2 (GTG GTG AAC TGC GTC-3 0 ) in the presence of complementary probe-conjugated TEX615.…”

Section: Determination Of Buffer Solution and Nanoparticle For Optimimentioning

confidence: 96%

“…13 Highly concentrated graphene oxide (GO, 500-700 nm, 5.5 mg ml À1 ) dispersed in water was purchased from Graphene Supermarket. We selected TEX615 as the uorescent dye used to label the complementary probe because its emission in the ODI-CL reaction has a higher intensity than other uorescent dyes (e.g., Texas Red, 6-FAM) widely utilized in conventional PO-CL or uorescence detection.…”

Section: Chemicals and Materialsmentioning

confidence: 99%

Rapid hybridization using graphene oxide and 1,1′-oxalyldiimidazole chemiluminescence

Choi¹,

Choi

Lee³

2013

RSC Adv.

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A rapid hybridization method using graphene oxide and highly sensitive 1,1 0 -oxalyldiimidazole chemiluminescence (ODI-CL) detection was developed from an understanding of the chemical and physical interactions between single strand DNA (ssDNA) molecules and nanoparticles (e.g., multi-walled carbon nanotubes, graphene oxide, gold and silver nanoparticles). The efficiency of hybridization between mutated ssDNA and a complementary probe conjugated with TEX615 was dependent on four different variables: pH, temperature, incubation time, and properties of nanoparticles capable of capturing excess complementary probes remaining after the hybridization. A critical problem observed when mutated ssDNAs rapidly bound with complementary probe-conjugated TEX615 was that three different types of possible mismatched ssDNAs also slowly and competitively hybridized with complementary probe-conjugated TEX615. The problem was solved upon addition of three different types of complementary probes, not conjugated with TEX615, in the solution because mismatched DNAs hybridize with their complementary probes while mutated ssDNAs bind with complementary probe-conjugated TEX615. The new hybridization method using graphene oxide and ODI-CL detection quantified trace levels of mutated ssDNAs in a sample containing mismatched ssDNAs within 15 minutes without any interference from mismatched ssDNAs.

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“…In order to confirm whether PSA aptamer-bound PSA emits light, we fabricated Fe 3 O 4 MWCNTs to remove free PSA aptamer, using the π-π stacking interaction between free aptamer and micro-or nano-particles (Cho et al, 2014;Choi et al, 2013;Park et al, 2013;Sheng et al, 2011;Yang et al, 2011) such as graphene oxide and MWCNTs, in aqueous solution based on the procedure shown in Fig. 3(b).…”

Section: Comparison Between Psa Aptamer 1 and Psa Aptamermentioning

confidence: 99%