Role of B7 Costimulatory Molecules in Mediating Systemic and Mucosal Antibody Responses to Attenuated<i>Salmonella enterica</i>Serovar Typhimurium and Its Cloned Antigen

García, Carlos; Martin, Michael; Michalek, Suzanne M.

doi:10.1128/iai.72.10.5824-5831.2004

Cited by 6 publications

(6 citation statements)

References 20 publications

(30 reference statements)

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“…These findings suggest that the ability of Kgp-HArep to preferentially increase the expression of CD86 on DC could in part affect the magnitude of the immune response to this antigen in a CD86-dependent manner. This would be in agreement with findings of others assessing the functional roles of CD80 and CD86 in mediating immunogenic properties of mucosally administered antigens and demonstrating a strong correlation between the ability of an antigen to selectively upregulate CD80 or CD86 on antigen-presenting cells and the preferential role the up-regulated costimulatory molecules play in mediating systemic and mucosal responses to the antigen [27,28,33].…”

Section: Discussionsupporting

confidence: 79%

“…Or perhaps this effect is due to the adjuvant, the antigen, the route of immunization, or a combination of these factors. It is most likely the latter possibility, since previous studies have reported a nonredundant role of CD80, as well as a compensatory role of CD80 and CD86 for inducing IgA antibody response following mucosal immunization with different antigens [27].…”

Section: Discussionmentioning

confidence: 95%

“…There is growing evidence that both the route of immunization and the type of adjuvant used play critical roles in influencing the nature of antigen-specific humoral immune responses [27,37,38]. However, the nature of the response can also be influenced by the antigen.…”

Section: Discussionmentioning

confidence: 99%

“…Past studies assessing the functional role of CD80 and CD86 in mediating the immunogenic properties of mucosally applied antigens have shown a strong correlation between the ability of the antigen to selectively up-regulate CD80 or CD86 on APC and the preferential role the up-regulated costimulatory molecule plays in mediating systemic and mucosal antibody responses to the antigen [27,28]. Since DC are the most potent APC [29], we next assessed the ability of Kgp-HArep to up-regulate CD80 and CD86 expression on murine bone marrowderived DC.…”

Section: Regulation Of Cd80 and Cd86 Expression On Dendritc Cells By mentioning

confidence: 99%

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Role of CD80 and CD86 in host immune responses to the recombinant hemagglutinin domain of Porphyromonas gingivalis gingipain and in the adjuvanticity of cholera toxin B and monophosphoryl lipid A

Zhang

Lewis

Michalek

et al. 2007

Vaccine

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The gingipains of Porphyromonas gingivalis have been implicated in the virulence of this bacterium, and antibodies to the hemagglutinin/adhesin domain (HArep) of the gingipains have been shown to protect against P. gingivalis colonization. However, the cellular mechanisms involved in host responses to HArep have not been elucidated. The purpose of the present study was to determine the functional role of CD80 and CD86 in mediating systemic and mucosal immune responses to the recombinant HArep derived from the gingipain Kgp (Kgp-HArep) after intranasal (i.n.) immunization. We also investigated the effect of the mucosal adjuvants the B subunit of cholera toxin (CTB) and monophosphoryl lipid A (MPL) on the functional role of the costimulatory molecules for the induction of systemic and mucosal responses to Kgp-HArep Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. NIH Public Access

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Regulation Of Cd80 and Cd86 Expression On Dendritc Cells By mentioning

confidence: 99%

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Role of CD80 and CD86 in host immune responses to the recombinant hemagglutinin domain of Porphyromonas gingivalis gingipain and in the adjuvanticity of cholera toxin B and monophosphoryl lipid A

Zhang

Lewis

Michalek

et al. 2007

Vaccine

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“…Low levels of B7 expression on APCs limits the response of activated T cells due to preferential signaling through the inhibitory receptor CTLA-4 (1,5,6). The importance of these costimulatory molecules in mucosal defenses was confirmed by studies where B7-1 and B7-2 knockout mice have reduced mucosal and systemic anti-Salmonella Ab responses (7).…”

mentioning

confidence: 48%

Subepithelial Myofibroblasts are Novel Nonprofessional APCs in the Human Colonic Mucosa

Saada¹,

Pinchuk²,

Barrera³

et al. 2006

The Journal of Immunology

107

137

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The human gastrointestinal mucosa is exposed to a diverse normal microflora and dietary Ags and is a common site of entry for pathogens. The mucosal immune system must respond to these diverse signals with either the initiation of immunity or tolerance. APCs are important accessory cells that modulate T cell responses which initiate and maintain adaptive immunity. The ability of APCs to communicate with CD4+ T cells is largely dependent on the expression of class II MHC molecules by the APCs. Using immunohistochemistry, confocal microscopy, and flow cytometry, we demonstrate that α-smooth muscle actin+, CD90+ subepithelial myofibroblasts (stromal cells) constitutively express class II MHC molecules in normal colonic mucosa and that they are distinct from professional APCs such as macrophages and dendritic cells. Primary isolates of human colonic myofibroblasts (CMFs) cultured in vitro were able to stimulate allogeneic CD4+ T cell proliferation. This process was dependent on class II MHC and CD80/86 costimulatory molecule expression by the myofibroblasts. We also demonstrate that CMFs, engineered to express a specific DR4 allele, can process and present human serum albumin to a human serum albumin-specific and DR4 allele-restricted T cell hybridoma. These studies characterize a novel cell phenotype which, due to its strategic location and class II MHC expression, may be involved in capture of Ags that cross the epithelial barrier and present them to lamina propria CD4+ T cells. Thus, human CMFs may be important in regulating local immunity in the colon.

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Monophosphoryl lipid A enhances nontypeable Haemophilus influenzae-specific mucosal and systemic immune responses by intranasal immunization

Iwasaki

Hirano

Kodama

et al. 2017

International Journal of Pediatric Otorhinolaryngology

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Role of B7 Costimulatory Molecules in Mediating Systemic and Mucosal Antibody Responses to AttenuatedSalmonella entericaSerovar Typhimurium and Its Cloned Antigen

Cited by 6 publications

References 20 publications

Role of CD80 and CD86 in host immune responses to the recombinant hemagglutinin domain of Porphyromonas gingivalis gingipain and in the adjuvanticity of cholera toxin B and monophosphoryl lipid A

Role of CD80 and CD86 in host immune responses to the recombinant hemagglutinin domain of Porphyromonas gingivalis gingipain and in the adjuvanticity of cholera toxin B and monophosphoryl lipid A

Subepithelial Myofibroblasts are Novel Nonprofessional APCs in the Human Colonic Mucosa

Monophosphoryl lipid A enhances nontypeable Haemophilus influenzae-specific mucosal and systemic immune responses by intranasal immunization

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