Role of azide in singlet oxygen reactions: Reaction of azide with singlet oxygen.

Hasty, Noel M.; Merkel, Paul B.; Radlick, Phillip; Kearns, David R.

doi:10.1016/s0040-4039(01)84236-2

Cited by 270 publications

(107 citation statements)

References 4 publications

Supporting

Mentioning

105

Contrasting

Order By: Relevance

“…1) at a more rapid rate than reported previously. For human D98/AH2 cells, 90% of the cells lost the ability to form clones within 25 min as compared to the 30 min reported earlier. In the case of mouse 3T6 and Chinese hamster V79 cells, the loss occurred by 50 and 80 min as compared to the earlier report of 90 and 120 min, respectively.…”

Section: Resultscontrasting

confidence: 50%

Effect of Near-Ultraviolet and Visible Light on Mammalian Cells in Culture II. Formation of Toxic Photoproducts in Tissue Culture Medium by Blacklight

Stoien

Wang

1974

Proc. Natl. Acad. Sci. U.S.A.

133

View full text Add to dashboard Cite

Near-ultraviolet radiation was found to be lethal for mammalian cells in Dulbecco's modified Eagle's medium without serum or phenol red. Irradiation of the cells with near-ultraviolet light while the cells were in phosphate-buffered-saline abolished the lethal effect. When only the medium was irradiated followed by the addition of unirradiated cells and serum, the cells were still killed. The photoactive components of the medium for this effect were riboflavin, tryptophan, and tyrosine. When riboflavin was deleted from the medium being irradiated and added later, almost no killing was detected. Irradiation of salt solution of riboflavin and tryptophan or riboflavin and tyrosine, resulted in cell killing. Little Irradiation of Cells. The light sources used in this study were two General Electric F15T8 BLB integral filter blacklight tubes with emission in the 300-to 420-nm range peaking at 365 nm (12). Light exposure was carried out in covered Falcon 3002 60-mm dishes placed approximately 20 cm below the light source. The lids of these dishes exclude light below 300 nm (6). Exposure intensity was 4 sW/mm2 at the cell surface, as measured by a Blak-ray meter J-221 (Ultraviolet Products, Inc.). Temperature was monitored and regulated to avoid any effect due to heating by the light source. The cells were trypsinized and plated in medium containing serum but no phenol red, 18 hr before exposure to blacklight. Twenty minutes before irradiation, the medium was removed and replaced with either 3.5 ml of phosphate-buffered saline containing NaCl, KC1, Na2HPO4, and KH2PO4; or MEM free of both phenol red and serum. The dishes were placed in the exposure chamber under 10% CO2 for 20 min before the light was turned on. At various intervals, dishes were removed from the light and kept in the dark in the same chamber. After the last dishes were removed from the light, serum was added to all dishes.Irradiation of Medium or Medium Components. Exposure conditions were similar to those of the last section except that no cells were present and only the aqueous solutions of medium components were exposed. The basic mixture (BaM) employed, to which other components were selectively added, contained NaCl, KC1, CaC12, MgSO4, NaH2PO4, Fe(NO3)3, glucose, inositol, cysteine, and glutamine. For the determination of the medium components responsible for photoproduct formation, other ingredients were added to BaM: (1) aminoacid solution (AA) containing arginine, isoleucine, leucine, lysine, methionine, threonine, valine, glycine, and serine; (2) vitamin solution (Vit) containing nicotinamide, thiamine, choline, D-pantothenic acid and pyridoxine; (3) one or more of the following compounds: histidine, phenylalanine, tryptophan, tyrosine, and riboflavin. After irradiation, the missing MEM ingredients and serum were added to the dishes fol-

show abstract

Section: Resultscontrasting

confidence: 50%

Effect of Near-Ultraviolet and Visible Light on Mammalian Cells in Culture II. Formation of Toxic Photoproducts in Tissue Culture Medium by Blacklight

Stoien

Wang

1974

Proc. Natl. Acad. Sci. U.S.A.

133

View full text Add to dashboard Cite

show abstract

“…All the following spectra were treated in the same way. The reaction between propofol and 1 O 2 was then checked by studying the spectral evolution of aerated solutions of RB-PPF mixtures in the presence of NaN 3 (an efficient singlet oxygen quencher) 30 and in D 2 O (known to increase 1 O 2 lifetime).…”

Section: -29mentioning

confidence: 99%

Investigation of singlet oxygen reactivity towards propofol

Heyne

Kohnen

Brault³

et al. 2003

Photochem Photobiol Sci

View full text Add to dashboard Cite

The reaction between the anaesthetic agent 2,6-diisopropylphenol (propofol, PPF) and singlet oxygen ( OD (75:25 v/v), respectively. The reaction of propofol with singlet oxygen produced by light irradiation of Rose Bengal leads essentially to two reaction products, 2,6-diisopropyl-p-benzoquinone and 3,5,3Ј,5Ј-tetraisopropyl-(4,4Ј)-diphenoquinone that are unambiguously identified from comparison with authentic samples.

show abstract

“…Our results suggest that the involvement of x 02 is not important. The reaction was not inhibited when 1 a was irradiated in the presence of sodium azide (up to c = 9 X 10-4 M) [13] or thiourea (0.05 M) [14]. These results were confirmed by comparing rate of conversion of la in D2O and H2O [15].…”

Section: £Er£-mentioning

confidence: 59%

Photolysis of Thiopurines in the Presence of Oxygen⁺

Wenska¹,

Paszỳc²

1981

Zeitschrift Für Naturforschung B

View full text Add to dashboard Cite

Abstract Irradiation (λ > 290 nm) of 6-thiopurine and some 9-substituted 6-thiopurines in oxygen saturated tert-butanol leads to hypoxanthine, purine and purine-6-sulfonic acid or their substituted derivatives. Purine and hypoxanthine formation can be sensitized with dyes. The reaction of 6-thiopurine with singlet oxygen has also been performed with purine and hypoxanthine among other products. It seems however that 1O2 is not an important intermediate in the photooxygenation. The photoreaction may have some synthetic value. Hypoxanthines (~50%) or purines (35-45%) were obtained depending on the solvent used.

show abstract

Role of azide in singlet oxygen reactions: Reaction of azide with singlet oxygen.

Cited by 270 publications

References 4 publications

Effect of Near-Ultraviolet and Visible Light on Mammalian Cells in Culture II. Formation of Toxic Photoproducts in Tissue Culture Medium by Blacklight

Effect of Near-Ultraviolet and Visible Light on Mammalian Cells in Culture II. Formation of Toxic Photoproducts in Tissue Culture Medium by Blacklight

Investigation of singlet oxygen reactivity towards propofol

Photolysis of Thiopurines in the Presence of Oxygen⁺

Contact Info

Product

Resources

About

Role of azide in singlet oxygen reactions: Reaction of azide with singlet oxygen.

Cited by 270 publications

References 4 publications

Effect of Near-Ultraviolet and Visible Light on Mammalian Cells in Culture II. Formation of Toxic Photoproducts in Tissue Culture Medium by Blacklight

Effect of Near-Ultraviolet and Visible Light on Mammalian Cells in Culture II. Formation of Toxic Photoproducts in Tissue Culture Medium by Blacklight

Investigation of singlet oxygen reactivity towards propofol

Photolysis of Thiopurines in the Presence of Oxygen+

Contact Info

Product

Resources

About

Photolysis of Thiopurines in the Presence of Oxygen⁺