Role of auxin and cytokinin in the regulation of the actin cytoskeleton in the in vitro germinating male gametophyte of petunia

Ковалева, Л. В.; Воронков, А. С.; Захарова, Е. В.

doi:10.1134/s1021443715020107

Cited by 15 publications

(18 citation statements)

References 28 publications

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“…6a) [14]. Pollen cultivation on the medium with the hormone resulted in the decrease in AF density along the length of the pollen tubes (Fig.…”

Section: Effects Of Plant Hormones On Acmentioning

confidence: 94%

“…As shown in Figs. 6a and 6b, an addition of 1 µM IAA to the cultivation medium led to the increase in the total amount of AF by 37% of their total pool in the pollen tube, being the greatest in both subapical and apical pollen tube zones known to be most important for polar tube growth [14]. In the case of ABA at the same concentrations, somewhat different results were obtained, and this was reflected in slight increase in total content of AF (up to 10%) and 15%-redistribution of AF to the apical zone of the tubes (Figs.…”

Section: Effects Of Plant Hormones On Acmentioning

confidence: 98%

“…Recently, it has been shown that petunia pollen grain germination in vitro is accompanied by changes in the levels of plant hormones, such as ethylene, indole-3-acetic acid (IAA), abscisic acid (ABA), gibberellins and cytokinins, and is sensitive to the treatment by exogenous ones [10][11][12]. Most recent data [11,13,14] allowed us to put forward a hypothesis about hormonal signaling in polar growth of pollen tubes. Addressing this problem, the two important circumstances were taken into account.…”

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confidence: 99%

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Regulation of Petunia Pollen Tube Growth by Phytohormones: Identification of Their Potential Targets

Ковалева¹,

Воронков²,

Захарова³

et al. 2016

JAST-A

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Abstract:It is known that cytoskeleton-dependent trafficking of cell wall and membrane components to apical plasma membrane (PM) coupled with ion transport across pollen PM is crucial for maintaining polar pollen tube growth. To elucidate whether plant hormones are involved in these processes, the effects of exogenous phytohormones, indole-3-acetic acid (IAA), abscisic acid (ABA), gibberellin A 3 (GA 3 ) and cytokinin (kinetin) on the growth, PM polarization, actin cytoskeleton (AC) organization and cytoplasmic pH (pH c ) of in vitro 4 h-growing petunia pollen tubes were investigated. IAA, ABA and GA 3 displayed the growth-stimulating effects and these were accompanied by orthovanadate-sensitive hyperpolarization of the PM. Fluorescent labeling the enzyme with H + -ATPase antibodies exhibited IAA-and ABA-induced lateral PM redistribution of it into the subapical zone of pollen tube PM. Pollen cultivation on the medium with latrunculin B, the inhibitor of actin polymerization, resulted in inhibition of pollen tube growth and simultaneously in the drop of endogenous IAA content. The IAA-growth stimulating effect was correlated with increased content of actin filaments (AF) in both apical and subapical zones of tubes, while ABA and GA 3 exerted the same effect but it was accompanied by redistributing F-actin only to apical zone. In contrast, kinetin decreased the total F-actin content and inhibited pollen tube growth. It has been shown that the рН c of growing pollen tubes is sensitive to the plant hormones. In the case of male gametophyte growing for 1, 2 and 4 h, IAA induced alkalinization of the cytosol, while ABA and GA 3 exerted qualitatively similar effect only after its growth for 1 h and 4 h, respectively. Kinetin, in contrast, resulted in acidification of the cytosol. All these results, taken together, indicate, for the first time, potential targets of the phytohormone action in pollen tubes.

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“…6a) [14]. Pollen cultivation on the medium with the hormone resulted in the decrease in AF density along the length of the pollen tubes (Fig.…”

Section: Effects Of Plant Hormones On Acmentioning

confidence: 94%

Section: Effects Of Plant Hormones On Acmentioning

confidence: 98%

mentioning

confidence: 99%

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Regulation of Petunia Pollen Tube Growth by Phytohormones: Identification of Their Potential Targets

Ковалева¹,

Воронков²,

Захарова³

et al. 2016

JAST-A

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“…The involvement of auxin in plant-microbe signaling is also known ). Recently, Kovaleva et al ( 2015 ) observed that the addition of IAA to the nutrient medium increased the content of actin cytoskeleton (F-actin) in the apical and subapical zones of pollen tubes that might be responsible for the stimulation of pollen growth. This argument is supported by further observations such as the decrease in the content of endogenous IAA, inhibited germination, and/or blocked male gametophyte polar growth.…”

Section: Positive Effectsmentioning

confidence: 99%

Plant, Soil and Microbes

2016

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“…The involvement of auxin in plant-microbe signaling is also known (Berg 2009 ). Recently, Kovaleva et al ( 2015 ) observed that the addition of IAA to the nutrient medium increased the content of actin cytoskeleton (F-actin) in the apical and subapical zones of pollen tubes that might be responsible for the stimulation of pollen growth. This argument is supported by further observations such as the decrease in the content of endogenous IAA, inhibited germination, and/or blocked male gametophyte polar growth.…”

Section: Positive Effectsmentioning

confidence: 99%

Role of Phytohormones in Stress Tolerance of Plants

Nadeem

Ahmad

Zahir³

et al. 2016

Plant, Soil and Microbes

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Environmental stresses, both biotic and abiotic, cause negative impact on plant growth and development, and plants need to adopt certain strategies for maintaining proper growth under stress conditions. These strategies include certain physiological, biochemical, and molecular mechanisms to cope with these stresses. These mechanisms include the production of hormones (phytohormones) and osmolytes. Phytohormones are organic molecules that affect various plant physiological processes like growth, development, and cell differentiation. Phytohormones regulate key physiological events under normal and stress conditions. They play a vital role for enhancing the ability of plants to adapt to the harsh environmental conditions by mediating a wide range of adaptive responses. These responses enable the plants to acclimatize to adverse soil conditions. Various types of phytohormones play an important function in plants individually or in coordination with each other. The nature and level of these hormones in plants are major factors that infl uence plant processes and functions. The present chapter describes the potential role of phytohormones for promoting plant growth and development under stress conditions. The major classes of plant hormones and their source of production have been described. Metabolism of phytohormones and their physiological responses with special reference to their concentration-dependent or negative impact on plant growth have been discussed in detail. The impact of these hormones on plant growth under stress conditions has been reviewed and discussed with selected examples. Also, the role of microbes in phytohormone production has been elaborated with examples. Future perspectives of the area have also been discussed.

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Role of auxin and cytokinin in the regulation of the actin cytoskeleton in the in vitro germinating male gametophyte of petunia

Cited by 15 publications

References 28 publications

Regulation of Petunia Pollen Tube Growth by Phytohormones: Identification of Their Potential Targets

Regulation of Petunia Pollen Tube Growth by Phytohormones: Identification of Their Potential Targets

Plant, Soil and Microbes

Role of Phytohormones in Stress Tolerance of Plants

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