Role of association of OmpK35 and OmpK36 alteration and blaESBL and/or blaAmpC genes in conferring carbapenem resistance among non-carbapenemase-producing Klebsiella pneumoniae

Hamzaoui, Zaineb; Ocampo-Sosa, Alain A.; Martínez, Marta Fernández; Landolsi, Sarrah; Ferjani, Sana; Maamar, Elaa; Saïdani, Mabrouka; Slim, Aminé; Martı́nez-Martı́nez, Luis; Boubaker, Ilhem Boutiba-Ben

doi:10.1016/j.ijantimicag.2018.03.020

Cited by 101 publications

(70 citation statements)

References 42 publications

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“…ANI analyses with representative strains of the seven phylogenetic groups of K. pneumoniae [52] showed that eight of the neonatal isolates were K. pneumoniae [98. [83][84][85][86][87][88][89][90][91][92][93][94][95][96][97][98].98 % ANI with K. pneumoniae ATCC 13883 T (GCA_000742135)] and one (#91) was K. quasipneumoniae [98.5 % ANI with K. quasipneumoniae subsp. quasipneumoniae 01A030 T (GCA_000751755)].…”

Section: Whole-genome Sequencing Of Neonatal Faecal Lpementioning

confidence: 99%

Preterm infants harbour diverse Klebsiella populations, including atypical species that encode and produce an array of antimicrobial resistance- and virulence-associated factors

et al. 2020

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Klebsiella spp. are frequently enriched in the gut microbiota of preterm neonates, and overgrowth is associated with necrotizing enterocolitis (NEC), nosocomial infections and late-onset sepsis. Little is known about the genomic and phenotypic characteristics of preterm-associated Klebsiella , as previous studies have focused on the recovery of antimicrobial-resistant isolates or culture-independent molecular analyses. The aim of this study was to better characterize preterm-associated Klebsiella populations using phenotypic and genotypic approaches. Faecal samples from a UK cohort of healthy and sick preterm neonates (n=109) were screened on MacConkey agar to isolate lactose-positive Enterobacteriaceae . Whole-genome sequences were generated for Klebsiella spp., and virulence and antimicrobial resistance genes identified. Antibiotic susceptibility profiling and in vitro macrophage and iron assays were undertaken for the Klebsiella strains. Metapangenome analyses with a manually curated genome dataset were undertaken to examine the diversity of Klebsiella oxytoca and related bacteria in a publicly available shotgun metagenome dataset. Approximately one-tenth of faecal samples harboured Klebsiella spp. ( Klebsiella pneumoniae , 7.3 %; Klebsiella quasipneumoniae , 0.9 %; Klebsiella grimontii , 2.8 %; Klebsiella michiganensis , 1.8 %). Isolates recovered from NEC- and sepsis-affected infants and those showing no signs of clinical infection (i.e. ‘healthy’) encoded multiple β-lactamases. No difference was observed between isolates recovered from healthy and sick infants with respect to in vitro siderophore production (all encoded enterobactin in their genomes). All K. pneumoniae , K. quasipneumoniae , K. grimontii and K. michiganensis faecal isolates tested were able to reside and persist in macrophages, indicating their immune evasion abilities. Metapangenome analyses of published metagenomic data confirmed our findings regarding the presence of K. michiganensis in the preterm gut. There is little difference in the phenotypic and genomic characteristics of Klebsiella isolates recovered from healthy and sick infants. Identification of β-lactamases in all isolates may prove problematic when defining treatment regimens for NEC or sepsis, and suggests that healthy preterm infants contribute to the resistome. Refined analyses with curated sequence databases are required when studying closely related species present in metagenomic data.

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Section: Whole-genome Sequencing Of Neonatal Faecal Lpementioning

confidence: 99%

Preterm infants harbour diverse Klebsiella populations, including atypical species that encode and produce an array of antimicrobial resistance- and virulence-associated factors

et al. 2020

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“…Klebsiella pneumoniae produces two main porins (OmpK35 and OmpK36) (102). While most clinical isolates of K. pneumoniae express both the OmpK35 and OmpK36 porins, most ESBL-producing K. pneumoniae clinical isolates produce only OmpK36 (103). Loss of porins is one of the factors contributing to antimicrobial resistance and may favor the selection of additional mechanisms of resistance.…”

Section: Porin Detectionmentioning

confidence: 99%

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for the Rapid Detection of Antimicrobial Resistance Mechanisms and Beyond

2018

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SUMMARY Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) has been successfully applied in recent years for first-line identification of pathogens in clinical microbiology because it is simple to use, rapid, and accurate and has economic benefits in hospital management. The range of clinical applications of MALDI-TOF MS for bacterial isolates is increasing constantly, from species identification to the two most promising applications in the near future: detection of antimicrobial resistance and strain typing for epidemiological studies. The aim of this review is to outline the contribution of previous MALDI-TOF MS studies in relation to detection of antimicrobial resistance and to discuss potential future challenges in this field. Three main approaches are ready (or almost ready) for clinical use, including the detection of antibiotic modifications due to the enzymatic activity of bacteria, the detection of antimicrobial resistance by analysis of the peak patterns of bacteria or mass peak profiles, and the detection of resistance by semiquantification of bacterial growth in the presence of a given antibiotic. This review provides an expert guide for MALDI-TOF MS users to new approaches in the field of antimicrobial resistance detection, especially possible applications as a routine diagnostic tool in microbiology laboratories.

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“…There are four main loss-of-function mutations that affect accumulation of antibacterial drugs in K. pneumoniae clinical isolates: loss of the transcriptional repressors OqxR (2,3) and RamR (4,5), and loss of the porins OmpK35 (22,23) and OmpK36 (24,25). Starting with a laboratory workhorse K. pneumoniae strain, Ecl8, we determined the influence of these mutations on meropenem MIC in the presence or absence of 8 μg.mL −1 vaborbactam when the strains carried one of two carbapenemases commonly encountered in the clinic: the OXA-48-like enzyme OXA-232 (26) or the class A carbapenemase KPC-3 (15).…”

Section: Resultsmentioning

confidence: 99%

Resistance to Ceftazidime/Avibactam Plus Meropenem/Vaborbactam When Both are Used Together Achieved in Four Steps From Metallo-β-Lactamase NegativeKlebsiella pneumoniae

Dulyayangkul

Douglas

Lastovka

et al. 2020

Preprint

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Role of association of OmpK35 and OmpK36 alteration and blaESBL and/or blaAmpC genes in conferring carbapenem resistance among non-carbapenemase-producing Klebsiella pneumoniae

Cited by 101 publications

References 42 publications

Preterm infants harbour diverse Klebsiella populations, including atypical species that encode and produce an array of antimicrobial resistance- and virulence-associated factors

Preterm infants harbour diverse Klebsiella populations, including atypical species that encode and produce an array of antimicrobial resistance- and virulence-associated factors

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for the Rapid Detection of Antimicrobial Resistance Mechanisms and Beyond

Resistance to Ceftazidime/Avibactam Plus Meropenem/Vaborbactam When Both are Used Together Achieved in Four Steps From Metallo-β-Lactamase NegativeKlebsiella pneumoniae

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