Role of apoptosis and Fas/FasL system in the oogenesis of the spotted ray <i>Torpedo marmorata</i>

Ricchiari, Loredana; Barbara, D'onghia; Liguoro, Annamaria; Tammaro, S.; Motta, Chiara Maria; Filosa, Stefania; Andreuccetti, Piero; Prisco, Michele De

doi:10.1002/mrd.10324

Cited by 8 publications

(2 citation statements)

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“…on August 28, 2018 by guest http://cvi.asm.org/ enous ray Torpedo, the bony fish Ictalurus and Sparus, and the amphibian Xenopus (17,(38)(39)(40)49), the present study established for the first time that reptile cells express a CD95 homolog which can be imaged and quantitated by using antibodies against mammalian CD95. In addition, incubation with activating ␣-CD95 IgM CH11 against mammalian CD95 induced reptilian fibroblasts to undergo apoptosis, which could be imaged by immunofluorescence and quantitated by TUNEL assay, evidence that the relationship of CD95-mediated signal transduction with apoptosis is the same in alligator fibroblasts as in other cell types.…”

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Mycoplasma alligatorisInfection Promotes CD95 (FasR) Expression and Apoptosis of Primary Cardiac Fibroblasts

Hunt

Brown

2005

Clin Vaccine Immunol

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Mycoplasma alligatoris causes acute lethal primary infection of susceptible hosts. A genome survey implicated sialidase and hyaluronidase, potential promoters of CD95-mediated eukaryotic cell death, as virulence factors of M. alligatoris. We used immunofluorescence imaging and flow cytometry to examine the effects of M. alligatoris infection in vitro on CD95 expression and apoptosis by alligator cardiac fibroblasts, a major cell type of a target organ of M. alligatoris infection in vivo. A uniform distribution of CD95 in primary cultured cardiac, skeletal muscle, and embryonic fibroblasts was demonstrated by using polyclonal antibodies against the N or C terminus of mouse or human CD95. Anti-CD95 antibodies reacted on Western blots of fibroblast lysates with a band with the predicted apparent molecular weight of CD95, but soluble CD95 was not detected in plasma from control or M. alligatoris-infected alligators. The proportion of CD95-gated cardiac fibroblasts increased threefold (P < 0.01) 48 h after inoculation with M. alligatoris. Infection induced morphological changes in cardiac fibroblasts, including translocation of CD95 characteristic of apoptosis and an eightfold increase (P < 0.16) in 5-bromo-2-deoxyuridine (BrdU) incorporation measured in a terminal deoxynucleotide transferase dUTP nick end-labeling apoptosis assay. The proportion of BrdU-gated controls activated with agonistic immunoglobulin M against human CD95 also increased threefold (P < 0.03 for muscle). Heat-inactivated M. alligatoris and sterile M. alligatoris-conditioned culture supernatant had no effect. This is the first report of a CD95 homolog in the class Reptilia and establishes a new model that can be used to test the direct bacterial interaction with upstream components of the CD95 signal transduction pathway.In contrast to the usually subtle mycoplasmoses, Mycoplasma alligatoris causes acute lethal primary infection of susceptible hosts (7). Pathological changes in affected individuals reflect multisystem inflammatory disease, including necrotic epicarditis, pericarditis, and myocarditis (8, 9). A comparative genome survey approach to the identification of candidate virulence mechanisms revealed that M. alligatoris strain A21JP2 T possesses genes for the "spreading factors" sialidase (nanI) and hyaluronidase (nagH), a combination unprecedented among mycoplasmas (10). Functional studies demonstrated expression of the corresponding glycosidase activities, which might degrade host extracellular matrix (ECM) glycans to facilitate the rapid invasiveness of M. alligatoris infection (16,28,31,41,48). Its attenuated sibling species, Mycoplasma crocodyli strain MP145 T (43), also possesses hyaluronidase, so direct ECM damage alone seems insufficient to explain the particular virulence of M. alligatoris. M. crocodyli MP145 T does not possess sialidase (10). Desialylation of the eukaryotic cell death inducer CD95 (the fibroblast-associated receptor FasR) by sialidase substantially promoted CD95-mediated apoptosis in B-lineage leukemias and Jurk...

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confidence: 68%

Mycoplasma alligatorisInfection Promotes CD95 (FasR) Expression and Apoptosis of Primary Cardiac Fibroblasts

Hunt

Brown

2005

Clin Vaccine Immunol

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“…The results exposed show that atresia may occur in any developmental stage, even in oogonia (Ricchiari et al, 2003). In early follicles, there is no major evidence than the disorganization and resorption of the follicle.…”

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confidence: 77%

Morphological indicators of initial reproductive commitment in Mustelus schmitti (Springer 1939) (Chondrichthyes, Triakidae): folliculogenesis and ovarian structure over the life cycle

2014

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This work provides information about the sexual commitment and the folliculogenesis of the gatuzo, Mustelus schmitti. A total of 112 females of all maturity stages were fished in the Bahía Blanca estuary, between 2009 and 2010. The oogonia were present throughout the life cycle of the animals. The folliculogenesis follows a pattern similar to other elasmobranchs. The granulosa layer keeps monolayered throughout the folliculogenesis, but with two cell types in the vitellogenic follicle. The zona pellucida forms in the primordial follicles. The thecal system shows a connective inner layer and a glandular outer sheath. The microscopic beginning of the sexual commitment, indicated by the vitello hoarding, takes place in follicles from 500 micrometres, while the macroscopic evidence appears in follicles of 2500-3000 micrometres. The results presented in this study suggest that the fishery pressure may affect a susceptible range of sizes of the species, not previously considered and provides a biological framework for the development of fisheries policy.

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Expression of vitellogenin receptor in the ovarian follicles during the reproductive cycle of the spotted ray Torpedo marmorata Risso 1880

et al. 2011

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The aim of this investigation was to identify the encoding sequence of vitellogenin receptor gene (vtgr), and its expression during the oogenesis in the spotted ray, Torpedo marmorata, in different phases of reproductive cycle. From an ovarian cDNA of vitellogenic female, we obtained a fragment of 581 bp, which corresponds to a partial sequence encoding the vitellogenin receptor (VTGR) in Torpedo (accession number: gi/193244760). This sequence shows a high identity with the VTGR of other vertebrates, particularly Leucoraja erinacea (89% identity) and Squalus acanthias (84% identity). We also showed that vtgr mRNA expression in the ovary modifies during the oogenesis and throughout the reproductive cycle. Indeed, in immature females, whose ovary contains only previtellogenic follicles, vtgr mRNA occurred in the oocyte cortex as well as within intermediate and pyriform cells. In mature females, whose ovary contains pre- and vitellogenic follicles, vtgr mRNA was detectable not only in the oocyte cortex and in intermediate and pyriform cells but also in small follicle cells present in the follicular epithelium of vitellogenic follicles. In ovulating females, that, as pregnant ones, show pre-and vitellogenic follicles, vtgr mRNA was evident in the oocyte cortex only, whereas in pregnant females, no vtgr mRNA was evident. The role of VTGR in the control of Torpedo vitellogenesis is discussed.

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Role of apoptosis and Fas/FasL system in the oogenesis of the spotted ray Torpedo marmorata

Cited by 8 publications

References 28 publications

Mycoplasma alligatorisInfection Promotes CD95 (FasR) Expression and Apoptosis of Primary Cardiac Fibroblasts

Mycoplasma alligatorisInfection Promotes CD95 (FasR) Expression and Apoptosis of Primary Cardiac Fibroblasts

Morphological indicators of initial reproductive commitment in Mustelus schmitti (Springer 1939) (Chondrichthyes, Triakidae): folliculogenesis and ovarian structure over the life cycle

Expression of vitellogenin receptor in the ovarian follicles during the reproductive cycle of the spotted ray Torpedo marmorata Risso 1880

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