Role of antioxidant defense during different stages of preadult life cycle in European corn borer (Ostrinia nubilalis, Hubn.): Diapause and metamorphosis

Abstract

Antioxidant enzymes, total glutathione (GSH), and ascorbic acid (ASA) were determined in whole body homogenates of nondiapausing larvae, diapausing larvae during the diapausing period (October, December, and February), and in pupae emerged from both diapausing and nondiapausing larvae of the European corn borer (Ostrinia nubilalis, Hubn., Lepidoptera: Pyralidae). The activities of catalase, selenium nondependent glutathione peroxidase (GPx), and glutathione-S-transferase (GST), as well as the content of GSH and ASA, were found to vary throughout the larval diapause. Compared to diapausing larvae, nondiapausing larvae were higher in levels of catalase, GPx, GST, and dehydroascorbate reductase (DHAR) activity. GSH content was also increased. However, nondiapausing larvae contained less ASA than diapausing ones. Pupae had higher GPx and GST activity and an increased ASA content compared to larvae. The pupae emerged from nondiapausing larvae had higher GST, glutathione reductase (GR), and DHAR activities, but lower GPx activity and ASA content than those emerged from diapausing larvae. Correlation analysis revealed differences in the way the antioxidant level is equilibrated for a particular stage and developmental pattern. The results suggest that cellular antioxidants are involved in both the protection of cells and the regulation of redox levels during the pre-adult stages of Ostrinia nubilalis. Arch. Insect Biochem. Physiol. 55:79-89, 2004.

Cold hardiness in Ostrinia nubilalis (Lepidoptera: Pyralidae): Glycerol content, hexose monophosphate shunt activity, and antioxidative defense system

“…It should be noted that the level of DHAR activity in November correlates well with a high level of GR activity in the same period. These two enzymes are functionally connected and are a part of the ascorbate recycling system in insects (Jovanovic-Galovic et al, 2004). On the other hand, DHAR activity in February was markedly lower in all experimental groups of Ostrinia larvae compared to the November sample.…”

Variations in antioxidant defense during the development of the solitary bee Osmia bicornis

“…Antioxidant enzymes play a significant role in scavenging ROS generated during pupal-adult metamorphosis, which induces programmed cell death and tissue remodeling (Noh et al 2010). According to Jovanovic-Galovic et al (2004), various components may be involved in antioxidant protection to preserve homeostasis in different stages of development. This observation is partially validated by the results of this study.…”

Anaerobic respiration and antioxidant responses of Corythucha ciliata (Say) adults to heat-induced oxidative stress under laboratory and field conditions

“…In most cases, when high concentrations of ROS are produced under oxidative stress, many important enzymatic antioxidants immediately act as scavengers to eliminate the surplus ROS generated in the cell (Jovanović-Galović et al 2004;Meng et al 2009;Zhou et al 2010;Wilczek et al 2013). SOD and CAT are regarded as the two most important antioxidant enzymes that reduce the high levels of superoxide anions and thereby reduce oxidative damage caused by environmental stresses (Park et al 2009;Bafana et al 2011;Celino et al 2011 Jovanović-Galović et al 2004).…”