Role of antibody to leukocytosis-promoting factor hemagglutinin and to filamentous hemagglutinin in immunity to pertussis

Abstract

Gamma globulins were obtained from rabbits hyperimmunized with the filamentous hemagglutinin (FHA) or the leukocytosis-promoting factor hemagglutinin (LPF. HA) of Bordetella pertussis. Double immunodiffusion analyses showed the antibodies to be highly specific for the homologous antigen. Intraperitoneal injection of anti-FHA or anti-LPF HA into 10-day-old ICR mice 30 min before aerosol challenge with B. pertussis protected the mice from disease as evidenced by survival and a normal rate of gain in body weight. Both anti-LPF HA and anti-FHA enhanced the clearance of B. pertussis from the lung and prevented leukocytosis which normally occurs after aerosol infection of mice with B. pertussis. Anti-LPF. HA, but not anti-FHA, neutralized the leukocytosis-promoting and histamine-sensitizing activities of LPF HA when incubated with the purified protein in vitro. Anti-FHA, but not anti-LPF-HA, prevented the attachment of B. pertussis to mammalian cells (HeLa and Vero) in culture.

Immunogenicity and protective efficacy of a recombinant filamentous haemagglutinin from Bordetella pertussis

“…There has been considerable debate whether FHA can confer protection against B. pertussis infection. Some murine studies have indicated that FHA is not protective [8,9], supporting human data that showed no correlation between the presence of anti-FHA antibodies and protection against household exposure to B. pertussis [10,11]. Other murine studies have shown that FHA is protective [12,13].…”

“…Earlier work by Sato et al . [8] and Sato and Sato [9] questioned the protective efficacy of FHA. In addition, Cherry et al .…”

Role of Pertussigen (Pertussis Toxin) on the Mouse Protective Activity of Vaccines Made from Bordetella Species

“…This latter observation has been confirmed by Sato et al (43). These experiments seem to show that the mouseprotecting activity of pertussis vaccine determined by the ic challenge test depends on antigens other than Ptx, although this toxin in small amounts is required (23,28,38,42). Ptx, when partially detoxified, protects mice at doses over 1,600 ng; in smaller nonprotective doses, Ptx acts as an adjuvant to other surface antigens of B. pertussis.…”

“…Detoxified Ptx has been selected as one of the components in these vaccines because it protects mice against intracerebral (ic) and aerosol induced infections with virulent B. pertussis (22,41,42,47), and because the toxin has been postulated to be responsible for the symptomatology of whooping cough (20,32,33,47). Furthermore, the mouse protection test, which employs the ic challenge (16), is a required test in various countries for determining the protective potency of pertussis vaccines (20,31), and vaccines without Ptx do not induce adequate protection against ic challenge in mice (23,28,38).…”

The virulence factors of Bordetella pertussis: a matter of control

“…The heparin binding activity of FHA may allow B. pertussis to bind to non-ciliated cells as demonstrated using WiDr cells, HeLa cells, and Vero cells [89,90], or perhaps facilitate interactions with the ECM. The lectin-like binding of FHA to heparin and dextran sulfate was character-ised when it was shown that binding to epithelial cells could be inhibited by the addition of heparin [91].…”