Role of Antibiosis in Competition of
            <i>Erwinia</i>
            Strains in Potato Infection Courts

Axelrood, Paige E.; Rella, Manuela; Schroth, M. N.

doi:10.1128/aem.54.5.1222-1229.1988

Cited by 33 publications

(16 citation statements)

References 26 publications

(24 reference statements)

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“…Previous studies have shown that oxygen is required for carbapenem production (26, 54). Therefore, to better understand the role played by oxygen in bacterial competition and the biological significance of carbapenem and bacteriocin production in the context of potato tubers, we assayed the ability of Pcb 1692 to produce these antimicrobial compounds under aerobic or anaerobic conditions.…”

Section: Resultsmentioning

confidence: 99%

The impact of type VI secretion system, bacteriocins and antibiotics on competition amongst Soft-RotEnterobacteriaceae: Regulation of carbapenem biosynthesis by iron and the transcriptional regulator Fur

Nkomo

et al. 2018

Preprint

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17Plant microbial communities' complexity provide a rich model for investigation on biochemical and 18 regulatory strategies involved in interbacterial competition. Within these niches, the soft rot 19 Enterobacteriaceae (SRE) comprise an emerging group of plant-pathogens inflicting soft rot/black-20 leg diseases and causing economic losses worldwide in a variety of crops. In this report, a range of 21 molecular and computational techniques are utilized to survey the contribution of antimicrobial 22Pcb1692 inhibits growth of other SRE and several potato endophytes using either the type VI 51 secretion, carbapenem or bacteriocins. Carbapenem plays a role in both inter and intrabacterial 52 competition in vitro, while the Pcb1692T6SS plays a role in interbacterial competition in planta (in 53 potato tubers). We also demonstrate that carbapenem regulation requires the presence of 54 environmental iron and oxygen in a complex network consisting of Pcb1692 Fur, SlyA, and ExpI. 55

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Section: Resultsmentioning

confidence: 99%

The impact of type VI secretion system, bacteriocins and antibiotics on competition amongst Soft-RotEnterobacteriaceae: Regulation of carbapenem biosynthesis by iron and the transcriptional regulator Fur

Nkomo

et al. 2018

Preprint

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“…There is some evidence that antibiotic production by Erwinia spp. is important for the elimination of sensitive Erwinia competitors from sites of infection in the plant host [186]. This fact, coupled with the commercial potential of this natural β‐lactam and the genetic tractability of Ecc , has meant that many research projects have focussed on the production of carbapenem in this organism.…”

Section: Quorum‐sensing Systems Beyond the Luxri Paradigmmentioning

confidence: 99%

Quorum-sensing in Gram-negative bacteria

Whitehead¹,

Barnard²,

Slater³

et al. 2001

FEMS Microbiol Rev

1,321

939

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It has become increasingly and widely recognised that bacteria do not exist as solitary cells, but are colonial organisms that exploit elaborate systems of intercellular communication to facilitate their adaptation to changing environmental conditions. The languages by which bacteria communicate take the form of chemical signals, excreted from the cells, which can elicit profound physiological changes. Many types of signalling molecules, which regulate diverse phenotypes across distant genera, have been described. The most common signalling molecules found in Gram-negative bacteria are N-acyl derivatives of homoserine lactone (acyl HSLs). Modulation of the physiological processes controlled by acyl HSLs (and, indeed, many of the non-acyl HSL-mediated systems) occurs in a cell density- and growth phase-dependent manner. Therefore, the term 'quorum-sensing' has been coined to describe this ability of bacteria to monitor cell density before expressing a phenotype. In this paper, we review the current state of research concerning acyl HSL-mediated quorum-sensing. We also describe two non-acyl HSL-based systems utilised by the phytopathogens Ralstonia solanacearum and Xanthomonas campestris.

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“…Competition assays were performed in potato tubers as described by Axelrood et al, 1998 (104). In summary, Solanum tuberosum (cv.…”

Section: Methodsmentioning

confidence: 99%

Horizontally acquired quorum sensing regulators recruited by the PhoP regulatory network expand host-adaptation repertoire in the phytopathogenPectobacterium carotovorum

Bellieny-Rabelo

Nkomo²,

Shyntum³

et al. 2019

Preprint

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In this study, we examine the impact of transcriptional network rearrangements driven by horizontal gene acquisition in PhoP and SlyA regulons using as a case study the phytopathosystem comprised of potato tubers and the soft rot pathogen Pectobacterium carotovorum subsp. brasiliense (Pcb1692). By comparing those two networks with that of PecS obtained from the closely related Dickeya dadantii, we found that: (a) 24-31% of the genes regulated at late infection are genus-specific (GS) (based on Pectobacterium and Dickeya genera), and that (b) of these, 28.1-44.4% were predicted with high confidence as horizontal gene transfer (HGT) candidates. Further, genome simulation and statistical analyses corroborated the bias in late infection regulons towards the transcriptional control of candidate GS-HGT genes by PhoP, SlyA, and PecS, highlighting the prominence of network rearrangements in these late infection regulons. The evidence further supports the circumscription of two horizontally acquired quorum sensing regulators (carR and expR1) by the PhoP network. By recruiting carR and expR1, the PhoP network also impacts certain host adaptation- and bacterial competition-related systems, seemingly in a quorum sensing-dependent manner, such as the type VI secretion system, carbapenem biosynthesis, and plant cell walls degrading enzymes (PCWDE) such as cellulases and pectate lyases. Conversely, polygalacturonases and the type III secretion system (T3SS) exhibit a transcriptional pattern that suggests quorum sensing-independent regulation by the PhoP network. This includes a yet uncharacterized novel phage-related gene family within the T3SS gene cluster that has been recently acquired by two Pectobacterium species. The evidence further suggests a PhoP-dependent regulation of carbapenem and PCWDE-encoding genes based on the synthesized products’ optimum pH. The PhoP network also controls slyA expression in planta, which seems to impact the carbohydrate metabolism regulation, especially at early infection when 69.6% of the SlyA-regulated genes from that category also require PhoP to achieve normal expression levels.AUTHOR SUMMARYExchanging genetic material through horizontal transfer is a critical mechanism that drives bacteria to efficiently adapt to host defenses. In this report, we demonstrate that a specific plant pathogenic species (from the Pectobacterium genus) successfully integrated a population density-based behaviour system (quorum sensing) acquired through horizontal transfer into a resident stress-response gene regulatory network controlled by the PhoP protein. Evidence found here underscores that subsets of bacterial weaponry critical for colonization, typically known to respond to quorum sensing, are also controlled by PhoP. Some of these traits include different types of enzymes that can efficiently break plant cell walls depending on the environmental acidity level. Thus, we hypothesize that PhoP ability to elicit regulatory responses based on acidity and nutrient availability fluctuations may have strongly impacted the fixation of its regulatory connection with quorum sensing. In addition, another global gene regulator known as SlyA was found under the PhoP regulatory network. The SlyA regulator controls a series of carbohydrate metabolism-related traits, which also seem to be regulated by PhoP. By centralizing quorum sensing and slyA under PhoP scrutiny, Pectobacterium cells added an advantageous layer of control over those two networks that potentially enhances colonization efficiency.

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Role of Antibiosis in Competition of Erwinia Strains in Potato Infection Courts

Cited by 33 publications

References 26 publications

The impact of type VI secretion system, bacteriocins and antibiotics on competition amongst Soft-RotEnterobacteriaceae: Regulation of carbapenem biosynthesis by iron and the transcriptional regulator Fur

The impact of type VI secretion system, bacteriocins and antibiotics on competition amongst Soft-RotEnterobacteriaceae: Regulation of carbapenem biosynthesis by iron and the transcriptional regulator Fur

Quorum-sensing in Gram-negative bacteria

Horizontally acquired quorum sensing regulators recruited by the PhoP regulatory network expand host-adaptation repertoire in the phytopathogenPectobacterium carotovorum

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